System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.

A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

This disclosure relates to an apparatus for simultaneously filling a plurality of sample chambers. In one aspect, the apparatus comprises a common fluid source and a plurality of independent, continuous fluidic pathways. Each independent, continuous fluidic pathway comprises a sample chamber and a pneumatic compartment. The sample chamber is connected to the common fluid source, and the pneumatic compartment is connected to the sample chamber. The sample chamber comprises, in part, an assay chamber. The assay chamber comprises a monolithic substrate and a plug having optically transmissive properties. In some embodiments, the assay chamber contains a magnetic mixing element. In some embodiments, the assay chamber is a double tapered chamber. In some embodiments, a ratio of a volume of the sample chamber to a volume of the pneumatic compartment is substantially equivalent for each fluidic pathway of the plurality of fluidic pathways.

Provided herein is a lateral flow diagnostic device and methods of using thereof. The device comprises a substrate and a first end, wherein the first end comprises a sample loading portion. The first end may further comprise a first region loaded with a detectable ligand, a CRISPR effector system, a detection construct, a first test band comprising a biotin ligand, and a second test band comprising a capture molecule for the detectable ligand. The detection construct may comprise an RNA oligonucleotide, having a first molecule such as FITC on a first end and a second molecule such as FAM on a second end. Contacting the sample loading portion with a sample causes the sample to flow from the sample loading portion of the substrate towards the first and second capture regions, thereby generating a detectable signal, which may be indicative of a disease state.

A system and method for ejecting one or more fluids from a digital dispense device. The method includes a) inputting to a memory a volume per unit area for each of the one or more fluids to be ejected from the digital dispense device; b) matching the volume per unit area to a device resolution for the digital dispense device; c) formatting fluid ejectors for the digital dispense device for the device resolution; and d) ejecting fluid from the digital dispense device to provide the volume per area for each of the one or more fluids.

A driving circuit, a driving method and a microfluidic substrate are provided. The driving circuit includes a first switching unit, a second switching unit, a reset unit, a first capacitor, and a second capacitor. In a first stage of a driving process of the driving circuit, the first switching unit is turned on, a first voltage signal is transmitted to a first node, the second switching unit is turned on, a second voltage signal is input to an output terminal of the driving circuit, and the driving circuit outputs an AC signal. In a second stage of the driving process, the first switching unit is turned off, the valid signal output by the second scan signal terminal controls the reset unit to be turned on, a third voltage signal is input to the output terminal of the driving circuit for reset, and the driving circuit outputs a DC signal.

Disclosed are methods and systems for confirming the identification of samples processed by a high-throughput analytical technique. The method may include the steps of distributing a plurality of individual samples to individual predetermined positions of a multi-sample test unit and distributing an identifier material to at least one predetermined position of the multi-sample test unit. The method may also include determining the absence or presence of an analyte in the plurality of individual samples, and determining the absence or presence of the identifier material, wherein the presence of the identifier material at the predetermined position of the multi-sample test unit is used to confirm the identity of the plurality of individual samples positioned in the multi-sample test unit. Also disclosed are systems for performing the disclosed methods or any of the steps of the disclosed methods. The methods and systems may be applied to high-throughput LC-MS/MS or other analytical methods.

Embodiments include a reaction vessel having a reaction chamber defined by opposing first and second interior-facing surfaces of the housing; a first light absorbing layer conforming to the first interior-facing surface of the housing component; and a second light absorbing layer conforming to the second interior-facing surface of the housing component; a first energy source configured to direct light through the housing at the first light absorbing layer; and a second energy source configured to direct light through the housing at the second light absorbing layer.

A flow cell applicator system can include a flow cell applicator including multiple flow cells to deposit multiple substance spots on a deposition surface, and a positioning assembly to position, to dock, and to unlock the multiple flow cells relative to the deposition surface. The substance spots can be depositable when the multiple flow cells are docked on the deposition surface. The flow cell applicator system can also include a spot deposition identifier operably associated with a processor to: record data related to substance spots as applied on the deposition surface, identify data related to substance spots previously deposited on the deposition surface, or both.

Microfluidic devices and methods for focusing components in a fluid sample are described herein. The microfluidic devices feature a microfluidic chip having a micro-channel having a constricting portion that narrows in width, and a flow focusing region downstream of the micro-channel. The flow focusing region includes a positively sloping bottom surface that reduces a height of the flow focusing region and sidewalls that taper to reduce a width of the flow focusing region, thereby geometrically constricting the flow focusing region. The devices and methods can be utilized in sex-sorting of sperm cells to improve performance and increase eligibility.