The present disclosure relates to paper microfluidic devices for use in combination with a viewing box assembly for imaging and rapid identification and quantification of target analytes in a fluid sample that is deposited onto the device such that one or more target analytes in the sample react with one or more diagnostic components on the paper, causing a detectable reaction. The reacted microfluidic device may then be placed inside an opaque viewing box having an internal light source and top panel viewing aperture through which the microfluidic device may be imaged using a mobile electronic device and graphical user-interface for purposes of detecting and quantifying the one or more target analytes. In some embodiments, the microfluidic device includes diagnostic paper and abase. In some embodiments, the microfluidic device includes a filter layer on top of diagnostic paper layer.

A method for identifying a patient as having a marker correlated with systemic lupus erythematosus (SLE) comprises obtaining a body fluid sample from a patient suspected of having SLE, analyzing miRNA expression in the obtained body fluid sample, and identifying the patient as having the marker correlated with SLE if an increase in expression of at least one miRNA selected from SEQ ID NOs: 1-160 and 243-402 and/or a decrease in expression of at least one miRNA selected from SEQ ID NOs: 161-242 and 403-484 compared to a body fluid sample obtained from a healthy individual is detected in the patient sample, or as not having the marker correlated with SLE if an increase in expression of at least one miRNA selected from SEQ ID NOs: 1-160 and 243-402 and/or a decrease in expression of at least one miRNA selected from SEQ ID NOs: 161-242 and 403-484 compared to a body fluid sample obtained from a healthy individual fails to be detected.

This disclosure provides devices and methods for the isolation of single cells or particles of interest from a solution comprising a plurality of cells or a solution composed of a homogenous population of particles. Specifically, the present disclosure is directed to microfluidic devices and methods for analyzing cells in a sample. More specifically, the present disclosure provides droplet microfluidic devices and methods for using the same to obtain (trap), encapsulate, and retrieve (isolate) single cells or particles from a sample with improved efficiency.

The present disclosure provides devices and methods for partitioning samples and analyzing analytes. The device may comprise one or more of a first plurality of first chambers and a second plurality of second chambers. A first chamber of the first plurality of chambers may have a first volume that is different from a second volume of a second chamber of the second plurality of chambers. The first plurality of chambers may comprise at least about 100 first chambers and the second plurality of chambers may comprise at least about 100 second chambers.

The present invention generally relates to a controlled fluidic device to develop spatially complex environments to enhance the rate of evolution in cell populations. The method further provides an enhanced understanding in the emergence, for example, drug resistance during cancer chemotherapy.

A device for manipulating microdroplets using optically-mediated electrowetting comprising: a first composite wall comprising: a first transparent substrate; a first transparent conductor layer on the substrate having a thickness of 70 to 250 nm; a photoactive layer activated by electromagnetic radiation in the wavelength range 400-1000 nm on the conductor layer having a thickness of 300-1000 nm; and a first dielectric layer on the conductor layer having a thickness of 120-160 nm; a second composite wall comprised of: a second substrate; a second conductor layer on the substrate having a thickness of 70 to 250 nm; and an A/C source to provide a voltage across the first and second composite walls connecting the first and second conductor layers; at least one source of electromagnetic radiation having an energy higher than the bandgap of the photoexcitable layer; and means for manipulating the points of impingement of the electromagnetic radiation on the photoactive layer.

A multiplexed lateral flow device includes an impermeable internal reservoir having an opening to receive a sample deposition. A fluid distributor pad is arranged in fluid communication with a lower surface of the internal reservoir. The fluid distributor pad includes a paper based microfluidic element having a pattern of a hydrophobic material to distribute a portion of the sample deposition substantially equally among a plurality of flow paths. Lateral flow assays having a plurality of flow lines are aligned with flow paths of the distributor pad. An impermeable top cover has a first window arranged over the opening of the internal reservoir, and at least a second window arranged over the test results of the lateral flow assays. A housing element houses the reservoir, the distributor pad and lateral flow assays. The housing element includes an impermeable bottom cover and a spacer element arranged between the top and bottom covers and, provides a gap between the lateral flow assays and the impermeable top cover.

A device for separating and/or isolating and/or washing target particles from a particles suspension. The device includes at least two inlets, at least two outlets, a container having a longitudinal axis and a chamber for fluid flow, being configured to be associated with a transducer, at least one transducer configured to generate bulk acoustic waves within the chamber, and at least one flow rate sensor configured to measure the flow rate of the fluid in the chamber. The inlets are located on one end of the container and the outlets are located on the other end along the longitudinal axis. The first and second inlet are each located on either side of the longitudinal axis, the first inlet and the second outlet are each located on either side of the longitudinal axis, and the second inlet and the first outlet are each located on either side of the longitudinal axis.

A perfusion device is for evaluating the risk of thrombotic and hemorrhagic diseases linked to blood coagulation processes. In particular, a cartridge is for conducting a dynamic ex-vivo evaluation method of the coagulation process in a subjects blood sample, including at least one perfusion chamber, a well for loading the blood sample and reagents, an inclined ascending microchannel connecting the well to a first end of the perfusion chamber and an inclined descending microchannel placed at a second end. Preferably at least one perfusion chamber includes two half-channels of different width, placed in series or in parallel, or the cartridge includes at least two perfusion chambers placed in parallel. At least two of the perfusion chambers have a different width.

The present disclosure relates to a microfluidic circuit comprising an inlet port; an outlet port; a main channel fluidically connecting the inlet port and the outlet port; and a series of dead-end microchambers of differing volumes, where each microchamber is individually fluidically connected to the main channel via a side channel. The present disclosure also relates to a microfluidic device comprising a support layer; a substrate layer disposed on the support layer; and one or more microfluidic circuits of the present disclosure, where the one or more circuits are disposed within the substrate layer. Also disclosed is a method for performing an assay.