Laboratory on chip and its layered manufacturing method, wherein the method includes: designing, by means of a computer program, a printed circuit (7), mixing and reaction cavities (3) of fluids, microchannels (2) and spaces (15) for the placement of electronic components to be found in each layer, mechanizing in one or more biocompatible substrates the different voids and passages that will make up the mixing and reaction cavities (3), microchannels (2), holes (8) that join the microchannels and spaces for the subsequent placement of electronic components (15), metallizing with a biocompatible conductive material those surfaces in which the printed circuit will be integrated (7) according to the design performed in the first step, generating the printed circuit (7) by photolithography and acid attack, bonding the electronic components in the corresponding spaces (15), joining all the layers that make up the final laboratory.

Fluid storage containers and analysis cartridges for use in assay processes are presented. In addition, systems comprising such storage containers and analysis cartridges and methods of using such containers and cartridges are presented as well. In specific embodiments, fluid storage containers are configured to be coupled to analysis cartridges in a first stage and a second stage.

This application provides fluidic devices, such as microfluidic devices, which can be used for the creation and/or manipulation of droplets in droplet-based microfluidic systems, as well as systems and methods for using the same. The microfluidic devices can be used to generate droplets, extract or inject volume to droplets, and/or split droplets. Also provided are methods for generating nucleosomes, and isolated DNA from nucleosomes (or from non-nucleosomes), for example using the disclosed devices.

Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.

A process and system for efficiently producing reference data that can be fed into a predictive model for predicting quality attribute concentrations in cell culture processes. A perfusion bioreactor is operated at pseudo-steady-state conditions and one or more attribute influencing parameters are manipulated and changed over time. As the one or more attribute influencing parameters are manipulated, one or more quality attributes are monitored and measured. In one embodiment, multiple quality attributes are monitored and measured in parallel. The quality attribute information is recorded in conjunction with the changes in the attribute influencing parameters. This information is then fed to the predictive model for propagating cell cultures in commercial processes and maintaining the cell cultures within desired preset limits.

The present invention generally relates to systems and methods to create stable emulsions with low rates of exchange of molecules between microdroplets.

Engineered nanoscale multicomponent particles are introduced and are called “quants.” Methods and apparatuses for producing such multicomponent nanoparticles are provided. A single quant can be manufactured to contain a variety of different internal component molecules. Likewise, a plurality of such quants may be manufactured wherein the plurality of quants are suspended in an aqueous solution. Typically, quants are produced in quantity and concentration adequate to support human scale therapeutics. In some embodiments, millions or billions of quants are suspended in a volume of aqueous solution for delivery to a patient. When manufactured to the same specification, the plurality of quants are uniform in size, uniform in chemical composition, and therefore uniform in functionality. Functional uniformity is an essential aspect of quants, manifested in design and production. By controlling the variables of manufacture, such as particle size and composition, and by redefining a drug dose as the measured number of quants delivered (as opposed to measuring a drug dose by the mass of its active ingredient), the performance of these nanoparticle-based drugs introduce significant efficiencies and much higher value products to the expanding therapeutics market.

Beads with functionalized material applied to them are exposed to an acoustic field to trap, retain or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes. The acoustic field may be generated, for example, in an acoustic angled wave device or in an acoustic fluidized bed.

The device comprises a nib (12) having a working surface (16) exposed or exposable for acquiring a biological sample and a porous structure to absorb the sample thus acquired. A reservoir (28) provides fluid under pressure to the nib via a valve (29), conveying and dispensing the sample into a reaction chamber (14), where it reconstitutes a dried-down reagent (43) to perform an analytic reaction on the sample, for example, an isothermal amplification of nucleic acid released from the sample by a membrane-disrupting agent pre-functionalised in the porous nib. The nib may be initially mounted (A) in the outlet of the reservoir or (B) in the inlet to the reaction chamber, in either case with its working surface (16) initially exposed to acquire the sample before the components of the device are assembled for performing the analytical procedure.

A biopsy container assembly having a container with a lower chamber suitable for containing a buffer liquid, and a cap coupled with the container. The cap being provided with a chamber that contains a biopsy liquid sealed by a membrane, and a crown with a toothing-suitable for tearing off the membrane of the cap. The crown is also suitable for being disposed in the container in an initial position, wherein the toothing is directed downwards in order not to interfere with the membrane of the cap when the cap is closed on the container, and in an operating position, wherein the toothing is directed upwards in order to tear off the membrane of the cap when the cap is closed on the container.