Embodiments include assay devices within a single use, disposable cassette for biochemical assays with at least one fluid well and at least one capillary valve. The capillary valve in combination with motion of porous sheets within the assay leads to addition of liquid reagents at different times to the assay although a user adds the liquid reagents to the cassette simultaneously.

Air-matrix digital microfluidics (DMF) apparatuses and methods of using them to prevent or limit evaporation and surface fouling of the DMF apparatus. In particular, described herein are air-matrix DMF apparatuses and methods of using them in which a separate well that is accessible from the air gap of the DMF apparatus isolates a reaction droplet by including a cover to prevent evaporation. The cover may be a lid or cap, or it may be an oil or wax material within the well. The opening into the well and/or the well itself may include actuation electrodes to allow the droplet to be placed into, and in some cases removed from, the well. Also described herein are air-matrix DMF apparatuses and methods of using them including thermally controllable regions with a wax material that may be used to selectively encapsulate a reaction droplet in the air gap of the apparatus.

A fabric based microfluidic point of care at home diagnostic system is disclosed. The system comprising a fabric substrate one or more hydrophobic threads bound with one r more hydrophilic threads by means of weaving, knitting, embroidering, or sewing. The fabric is configured to define a flow path for a sample to flow from an introduction zone, to a preparation zone, to a testing zone, in a pattern sufficient to optimize the sample analysis required for sample diagnostic tests. The system further comprises one or more mechanical stages and one or more fluid cartridges. The mechanical stages comprise electrical and/or analytical equipment configured to record, detect, analytes or facilitate chemical reactions and/or condition of the air above the fabric to ensure sufficient for analysis. The fluid cartridges are attached to the edge of the fabric in certain zones to supply the fabric with reagents required for analysis in that zone.

The present invention provides system and methods for detecting an analyte indicative of an influenza viral infection in a sample of bodily fluid. The present invention also provides for systems and method for detection a plurality of analytes, at least two of which are indicative of an influenza viral infection in a sample of bodily fluid.

Provided is a micro- and nano-fluidic chip, including at least one nanochannel array layer and at least one microchannel array layer that are alternately stacked. The at least one nanochannel array layer includes nanochannels, the at least one microchannel array layer includes input units and/or output units. The input unit includes inlet microchannel arrays and inlets, and the output unit includes outlet microchannel arrays and outlets. The inlet microchannel array includes inlet microchannels, the outlet microchannel array includes outlet microchannels, and the inlet microchannels and the outlet microchannels are connected through the nanochannels.

A multi-level microfluidic device is provided. The device includes a silicon wafer substrate and a stack of layers arranged on the silicon wafer substrate. The stack comprises a plurality of fluidic silicon layers, wherein each fluidic silicon layer includes a microfluidic structure at least one intermediate layer. The at least one intermediate layer is arranged between two fluidic silicon layers, and a fluid inlet and a fluid outlet in fluid connection with at least one of the fluidic silicon layers. Each layer in the stack is formed by deposition or growth. Methods for manufacturing microfluidic devices is also provided.

An embodiment is a scientific fluidic device and a method of assembly of single and multilayer fluidic devices via laser cut and assembly of double sided adhesives. The device includes a member defining a cavity and having two sides, both sides including an adhesive compound, and at least one substrate defining at least two plenums and coupling to the member, forming a flow path. The components of the fluidic device are produced via laser cut and assembly methods. The fluidic device remains intact via adhesive coupling between the substrate(s), member(s), and membrane(s). Altogether, the fluidic device requires assembly that is efficient and economical, resulting in high throughput manufacturing of the fluidic devices.

There are provided a detection sensor, a detection sensor kit, a sensor device, a method for producing a detection sensor and a detection method which enable measurement by a simple operation. A detection sensor includes a detecting element, a first member and a second member. The detecting element detects a detection object contained in a sample. The first member includes a first channel portion, and the first channel portion includes a first storage section which stores a reagent which is fed to the detecting element. The second member is movable relative to the first member and includes a second channel portion. In the detection sensor, the first member and the second member are joined with each other and form a joined channel in which the first channel portion and the second channel portion communicate with each other.

A microfluidic cell culture system is provided. The system includes a dual circulation arrangement for providing the cell culture with culture medium (and, optionally, selected compounds for study). The dual circulation arrangement permits culture conditions to be readily modified for different phases of cell culture. In particular, a first circulation route can be used to circulate a relatively high volume of medium, thereby allowing a low cell number to medium volume ratio, and a second circulation route can be used to circulate a relatively low volume of medium, thereby allowing a high cell number to medium volume ratio. The first circulation is optimised for a pre-culture period, before test compounds are added, and the second circulation is optimised for the test phase, providing a high cell number to medium ratio while preserving function during the test period.

A particle separating and measuring device of the present disclosure includes: a first flow path device including a post-separation flow outlet through which a first fluid containing specific particles to be separated flows out; and a second flow path device on which the first flow path device is placed and including a first flow inlet through which the first fluid flows in, the first flow path device in which the post-separation flow outlet is arranged in a lower surface is placed on the second flow path device in which the first flow inlet is arranged in an upper surface of a first region, the post-separation flow outlet and the first flow inlet are connected so as to face each other, and a size of an opening of the first flow inlet is larger than a size of an opening of the post-separation flow outlet.