Examples herein provide a device. The device includes a sample delivery component, which includes: a reagent chamber to contain at least one reagent; a sample chamber to contain a fluid sample; and a delivery channel extending from the reagent chamber and in fluid communication with the sample chamber and an output port, wherein the delivery channel is conducive mixing the at least one reagent and the fluid sample to form a mixture before the mixture reaches the output port and be discharged therefrom. The device includes a testing cassette detachable from the delivery component, which includes: an input port in fluid communication with a microfluidic reservoir, the input port to receive the discharged fluid sample from the output port; and a micro-fabricated integrated sensor in a microfluidic channel extending from the microfluidic reservoir.

A microfluidic valve may include a first portion of a liquid conduit to contain a gas, a second portion of a liquid conduit to contain a liquid, and a constriction between the first portion and the second portion and across which a capillary meniscus is to form between the gas and the liquid. The microfluidic valve may further include a drop jetting device within the second portion to open the valve by breaking the capillary meniscus across the constriction.

Systems, methods, and apparatuses are provided for self-contained nucleic acid preparation, amplification, and analysis.

Disclosed are a detection chip and a manufacturing method therefor, and a reaction system. The detection chip includes: a first substrate (11); a microcavity defining layer (12), which is located on the first substrate (11) and defines a plurality of micro-reaction chambers (120); and a shading structure layer (13), which is located on the first substrate (11) and provided among the plurality of micro-reaction chambers (120). In practical application, the number of target molecules in a reaction system solution in each micro-reaction chamber (120) can be determined by collecting a fluorescence image; and the detection chip is provided with the shading structure layer (13), and the shading structure layer (13) is located on the first substrate (11) and provided among the plurality of micro-reaction chambers (120).

A detection chip, a method of using a detection chip and a reaction system are provided. The detection chip includes a first substrate, a micro-chamber definition layer and a heating electrode. The micro-chamber definition layer is located on the first substrate and defines a plurality of micro-reaction chambers. The heating electrode is located on the first substrate and closer to the first substrate than the micro-chamber definition layer, and configured to release heat after being energized. The heating electrode includes a plurality of sub-electrodes, orthographic projections of the plurality of micro-reaction chambers on the first substrate overlap with orthographic projections of at least two of the plurality of sub-electrodes on the first substrate, and the at least two of the plurality of sub-electrodes have different heating values per unit time after being energized.

The present disclosure provides a microfluidic chip including a plurality of microcavities, at least two of the plurality of microcavities have different volumes.

Disclosed are a substrate for a microfluidic device, a microfluidic device, a driving method of the microfluidic device, and a method of manufacturing a substrate for the microfluidic device. The substrate includes: a first base substrate; a first electrode layer on the first base substrate, the first electrode layer including a plurality of drive electrodes. The plurality of drive electrodes define at least one flow channel and at least one functional area in the first substrate, the at least one functional area includes a reagent area, the at least one flow channel includes a reagent area flow channel, the reagent area includes a reagent area liquid storage portion and a droplet shape changing portion, the droplet shape changing portion is adjacent to the reagent area flow channel, and the reagent liquid storage portion is on a side of the droplet shape changing portion away from the reagent area flow channel.

An apparatus includes a substrate, a first heating element, and a second heating element. The substrate includes a first portion, a second portion, and a third portion that is between the first portion and the second portion. The first portion is characterized by a first thermal conductivity, the second portion is characterized by a second thermal conductivity, and the third portion is characterized by a third thermal conductivity. The third thermal conductivity is less than the first thermal conductivity and the second thermal conductivity. The first heating element is coupled to the first portion of the substrate, and is configured to produce a first thermal output. The second heating element is coupled to the second portion of the substrate, and configured to produce a second thermal output. The second thermal output is different from the first thermal output.

A reaction processor includes: a reaction processing vessel including a channel in which a sample moves and a pair of air communication ports, a first air communication port and a second air communication port, provided at respective ends of the channel; a temperature control system that provides a medium temperature region and a high temperature region between the first air communication port and the second air communication port in the channel; and a liquid feeding system that discharges and sucks air in order to move and stop the sample inside the channel. One of the pair of air communication ports of the reaction processing vessel that is farther away from the high temperature region communicates with the liquid feeding system via a tube. One of the pair of air communication ports of the reaction processing vessel that is closer to the high temperature region is opened to atmospheric pressure.

High-throughput digital microfluidic (DMF) systems and methods (including devices, systems, cartridges, DMF apparatuses, etc.), are described herein. The systems, apparatuses and methods integrate liquid handling with the DMF apparatuses, providing flexible and efficient sample reactions and sample preparation. These systems, apparatuses and methods may be used with a variety of cartridge configurations and sizes.