A system and apparatus for precision temperature control of thermal bead baths used in biological laboratories to heat biological samples is disclosed. The system has an insulated outer shell and an inner shell sealed together to form a recirculation pathway. The inner shell has an air extraction port opening into the recirculation pathway and at least one air injection port opening into the recirculation pathway. A fan is in the recirculation pathway and is positioned to draw air through the air extraction port. At least one thermal sensor is connected to a control and is disposed in close proximity to one of the air injection ports. Beads used in thermal bead baths are placed in a mesh basket inside the inner shell. The fan draws air from the inner shell through the beads and into the recirculation pathway, where the air is heated by a thermal element. The air flows past the thermal element and through the air injection ports back into the inner shell.

The object of the invention is a device for conducting amplification reaction of biological samples with a system for independent control of the temperature of test tubes in a heating assembly comprising a multipart heating slot located in the cooling system housing, characterised in that the heating assembly comprises at least one heating slot (100) comprising a metal heating sleeve (101) wound around with a bifilar winding wire made of enamelled winding wire (102), which is covered with a composite polymer layer (103), wherein a temperature sensor (104) is located on the surface of the winding wire, and at least one heating slot is mounted on the PCB control board (105) located on the cooling system housing (112),

Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.

A recirculating bath includes a reservoir for receiving a working liquid, a recirculating pump, and at least one thermal element. The recirculating pump and thermal element are located externally to the reservoir so that the reservoir has an unobstructed working space. The thermal element may be thermally coupled to the working liquid through an interior surface of the reservoir, or the working liquid may be circulated over the thermal element by the recirculating pump in a chamber external to the reservoir. The recirculating bath may also include a lid that provides access to the reservoir by pivoting on a latching hinge. When open, the lid may provide a working surface adjacent to the reservoir. The lid may also include a selector that unlatches the hinge so that the lid can be removed. The recirculating pump may be fluidically coupled to the reservoir via a manifold.

The present disclosure provides a digital PCR system. The system includes a droplet formation assembly and a droplet orifice assembly. The droplet formation assembly includes a heat conducting plate and a cover plate, at least one inverted U-shaped step is placed on a side surface of the cover plate, the heat conducting plate, the cover plate and the inverted U-shaped step together form a droplet formation chamber having an opening at a bottom. The droplet orifice assembly is connected below the droplet formation assembly, and includes a plurality of droplet orifices, the droplet orifice is connected with the droplet formation chamber, and a vaporization component is placed in the droplet orifice, the vaporization component vaporizes a digital PCR solution in the droplet orifice and rapidly pushes the digital PCR solution into a droplet forming oil in the droplet formation chamber, to form a digital PCR droplet.

A thermo-gravimetric analysis system includes a chamber having an interior; and a sample crucible connected to and inside of the chamber, the sample crucible configured to hold a sample material. The system further includes a reference crucible connected to and inside of the chamber; and a metal organic framework (MOF) crucible connected to and inside of the chamber, separate from the sample crucible, the MOF crucible including an MOF material.

The present invention provides a refrigerating/heating device that efficiently refrigerates and heats while suppressing device costs. This refrigerating/heating device for efficiently heating and refrigerating is provided with: a refrigeration chamber; a Peltier-type cooler for supplying cold air to inside the refrigeration chamber; a heat radiation member for radiating Peltier heat; fans for air-cooling the heat radiation member; an exhaust duct through which waste heat from the fans and the heat radiation member passes; and an installation part to which a subject to be heated can be installed. The subject to be heated is installed in the waste heat flow path of the exhaust duct and heated.

The present invention relates to the field of temperature monitoring and controlling apparatus, more particularly, relates to a thermoelectric temperature controlled sample holder for biomedical analyzers used for the processing of biological samples. Specifically, in biomedical research, samples of single cell suspensions are loaded into a biomedical analyzer, e.g. a flow cytometer for analysis. Some flow cytometers are equipped with multi sample loaders that hold for instance microtiter plates which can contain multiple, up to thousands of samples. Processing of all samples is done sequentially, sample after sample and may take several hours between the first to the last sample. Depending on the type of analysis it may be useful to maintain the samples at a defined temperature for example, to maintain viability and integrity of the sample or to keep biological processes running at physiological temperatures. The present invention allows to maintain the sample holder at definable temperatures between 3° C. to 70° C.

A PCR amplification and product detection system is disclosed. The system utilizes a uniform and direct photonic heating subsystem to mediate reaction-by-reaction, high-throughput PCR amplification detectable by a fluorescence detection subsystem. Reaction-by-reaction temperature monitoring for dynamic feedback heat regulation is also disclosed. Also disclosed are methods for using the same.