The present invention relates to devices and methods for collecting liquid samples such as for removing a target liquid sample fraction from a larger liquid sample, e.g., from a biological sample. These devices and methods are particularly useful for rapidly and cost-effectively removing the buffy coat layer from an anticoagulated blood sample. These devices and methods have the advantage that the removal can be made on blood sample processed by ordinary centrifugation and do not require more complicated or costly processing techniques such as density gradient centrifugation.

Devices, kits, and their methods of use for lysing and/or purifying biological particles, e.g., nuclei are provided. One or more thixotropic layers can be employed in a vessel to purify biological particles. A device with sharp features may be employed to lyse biological particles or the contents thereof.

The present invention may provide a microfluidic device including a rotatable body; a first chamber positioned in a direction of an inner wall of the body; a second chamber positioned in a direction of an outer wall of the body from the first chamber; and a backflow prevention unit, and wherein a fluid is transferred from the first chamber to the second chamber, and wherein the backflow prevention unit prevents a backflow of the fluid from the second chamber to the first chamber.

A device and system for detecting aldehydes or ketones and, more particularly, a device and system, for detecting aldehydes or ketones, utilized in a rotating platform are provided.

A fluidic device for detecting a target molecule in a fluid sample comprising a blocking chamber in fluidic communication with a detection chamber forming a blocking-detection chamber pair, the blocking chamber provided with at least one reagent for binding a non-target molecule in the sample, the blocking chamber adapted to maintain at least a portion of the bound non-target molecule within the blocking chamber, the detection chamber comprising at least one reagent for binding a target-molecule such that the target-molecule may be detected. The device comprises a combination detection chamber adapted to receive at least one reagent for binding both target and non-target molecules such that the combination of target and non-target molecules may be detected by binding to a detector.

Described herein is a size-based asymmetric nanopore membrane (ANM) filtration technology for high-efficiency exosome isolation, concentration, and fractionation. The ANM design prevents exosome deformation, lysing, and fusion due to the strong external force and thus significant increases the yield (up to 92%) while preserving other advantages of size-based ultrafiltration. It also offers a unique feature of being able to flush the contaminating proteins from the exosomes. It offers higher throughput, yield, sample purity, concentration factor, and more precise size fractionation than current approaches.

The present embodiments relate to a fluid control device using centrifugal force. The fluid control device using centrifugal force includes a fluid control portion comprising a plurality of chambers and controlling a movement of a fluid inside the chamber; a lower fixing portion positioned on a lower portion of the fluid control portion and fixing the plurality of chambers; an upper fixing portion positioned an on upper portion of the fluid control portion and fixing the plurality of chambers; and a fastening member penetrating and fastening the lower fixing portion, the fluid control portion, and the upper fixing portion, wherein the plurality of chambers are disposed to face each other and placed on the lower fixing portion.

The present invention discloses a method for preparing a micro-channel array plate, comprising the steps of : (1) arranging a first optical fiber glass rod and a second optical fiber glass rod closely, melting the two glass rods into a whole at a high temperature to obtain a melted glass rod, drawing the melted glass rod at least one time into a longer and thinner glass rod than the melted glass rod, and cutting the drawn glass rod into small pieces to obtain a micro-channel array plate blank, wherein the corrosion resistance of the first optical fiber glass rod and the second optical fiber glass rod to the same corrosive liquid is different; (2) corroding the micro-channel array plate blank by a corrosive liquid to obtain a micro-channel array plate crude product with through holes; and (3) conducting hydrophobic treatment on the micro-channel array plate crude product to obtain the micro-channel array plate.

A portable motorized centrifugal system is optimized for low cost manufacture and operation. Separation of inhomogeneous fluid biological samples, such as liquid plasma from whole blood, is a common step in medical diagnostic tests. This system may enable remote separation where access to plug-in power sources are limited. The system may facilitate at-home testing. Due to biohazard concerns, the entire centrifugal apparatus portable and disposable, or the system includes one or more disposable elements within the interior of the centrifuge. Alternatively, the system may contain a module of higher value components that are re-usable after disinfection. Devices and methods for implementing centrifugal separation may include disk-shaped fluidic cartridges and tubes with reduced drag cross-section.

A nucleic acid amplification in-situ real-time detection system and method using a micro-fluidic chip through optical fiber sensing. The system includes a white light source, a detection optical path, a microfluidic chip and a spectrum acquisition, processing and display module, which are connected in sequence. The detection optical path is configured to transmit white light from the white light source to the micro-fluidic chip and transmit an optical signal made by the microfluidic chip to the spectrum acquisition, processing and display module. The micro-fluidic chip is configured to carry out biochemical reaction; the spectrum acquisition, processing and display module is configured to acquire the optical signal, analyze the signal and generate a visual biochemical reaction real-time dynamic-change signal curve. This microfluidic chip real-time detection device detects nucleic acid amplification information by using a white light interfered hyperspectral method, so fluorescence-labeled analyte and non-fluorescence-labeled analyte are detected.