A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.

In an example implementation, a method of microfluidic filtering includes activating a first fluid pump within a microfluidic channel to cause a forward flow of fluid through the microfluidic channel and through a filter of a filter loop. The filter loop intersects the microfluidic channel at a loop entry and at a loop exit. The method includes activating a second fluid pump to cause a reverse flow of fluid through the filter.

Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

The invention discloses a six-face heating high-temperature sterilization biological shaking table, comprises a box body and a transmission mechanism, one side of the box body is provided with an opening, the opening is provided with a door body, the box body is internally provided with an inner container, and the side surface of the inner container is composed of five-surface concentric-square-shaped high-temperature-resistant heating wires; the two rocking shafts are rotationally arranged in the box body, a supporting plate is rotationally arranged between the ends of the two rocking shafts, the other end of the belt is connected with the outer wall of the belt pulley in a winding mode, the end of the belt pulley is rotationally inserted into the inner side wall of the first mounting groove through a pin shaft, and the end of the pin shaft of the belt pulley rotationally extends into the box body.

Disclosed is a nucleic acid detection device which includes an installation unit on which a cartridge is installed, the cartridge including a chamber configured to store nucleic acid, a reagent for amplifying the nucleic acid and a dispersion medium for dispersing the nucleic acid and the reagent; a sample processing unit configured to produce, within the chamber, an emulsion in which droplets containing the nucleic acid and the reagent are dispersed in the dispersion medium; a temperature regulating unit configured to regulate a temperature of the cartridge to amplify the nucleic acid contained in the droplet; and a detection unit configured to detect a signal based on the nucleic acid amplified by temperature regulation by the temperature regulating unit.

A microfluidic device and method for producing and collecting single droplets of a first fluid, the device including a microfluidic platform having at least a droplet microchannel wherein is produced a flow of single droplets of the first fluid dispersed in a second fluid immiscible with the first fluid, the droplet microchannel having at least one inlet extremity and at least one outlet extremity for distributing the flow of droplets, the device further including: a collection device including a plurality of receiving areas adapted to collect at least one of the droplets, elements for changing the relative position of the collection device and the outlet of the microfluidic platform, elements for controlling the flow of droplets, and elements for synchronizing the flow of droplets at the outlet of the droplet microchannel and the relative movement of the collection device with regards to the microfluidic platform.

Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

A droplet generating method includes: providing a micro-pipe for dispensing a first liquid and a container containing a second liquid; providing a moving and locating device for positioning the micro-pipe over the container; providing a liquid driving device connecting to the micro-pipe through a connecting tube; providing a vibrating equipment connected to the micro-pipe for vibrating the micro-pipe; forming a relative periodic vibration between the micro-pipe and the container so that the outlet end of the micro-pipe is displaced to touch the second liquid in the container during a relative periodic vibration; and dispensing the first liquid in the micro-pipe out from the outlet end of the micro-pipe during the relative periodic vibration to generate a plurality of droplets of the first liquid in the second liquid which is induced by a force of the second liquid imposed on the first liquid at the outlet end.

A droplet generating apparatus includes: a micro-pipe having an outlet end and extending along a longitudinal axis; a liquid driving device; a connecting tube with its one end connected to the micro-pipe and the other end extending to the liquid driving device; a container positioned at least in-part below the micro-pipe and containing a second liquid; and a vibrating equipment connected to the micro-pipe and adapted to form a relative periodic vibration between the micro-pipe and the container in a perpendicular direction with respect to the longitudinal axis of the outlet end of the micro-pipe; wherein the vibrating equipment in coordination with the liquid driving device dispense the first liquid from the micro-pipe and form a plurality of droplets of the first liquid in the second liquid which is induced by a force of the second liquid imposed on the first liquid at the outlet end of the micro-pipe.

Described herein are systems and methods of for conducting various biological assays on arrays utilizing electrowetting on dielectric (EWOD). The systems and methods may process the biological sample, or plurality thereof, using at least one droplet. The droplet, or plurality thereof, may be manipulated using the systems and methods described herein. Further described herein are improvements to arrays for facilitating the execution of biological assays on the arrays.