There is provided a sample processing cartridge comprising a. a sample entry location; b. a closed sample processing chamber; c. a sample analysis location comprising a sample analysis well; d. a first channel fluidly connecting the sample entry location and the sample processing chamber; e. a second channel connecting the sample analysis location and the sample processing chamber, the second channel comprising a closed or closable second channel valve; wherein the sample processing chamber comprises a second channel port providing fluid connection between the second channel and the sample processing chamber, the second channel port being positioned in a sample accumulating region of the sample processing chamber. There is also provided a sample processing system comprising the cartridge, and methods of use of the cartridge and processing system in a sample processing assay.

An in vitro microfluidic intestine on-chip is described herein that mimics the structure and at least one function of specific areas of the gastrointestinal system in vivo. In particular, a multicellular, layered, microfluidic intestinal cell culture, which is some embodiments is derived from patient's enteroids-derived cells, is described comprising L cells, allowing for interactions between L cells and gastrointestinal epithelial cells, endothelial cells and immune cells. This in vitro microfluidic system can be used for modeling inflammatory gastrointestinal autoimmune tissue, e.g., diabetes, obesity, intestinal insufficiency and other inflammatory gastrointestinal disorders. These multicellular-layered microfluidic intestine on-chips further allow for comparisons between types of gastrointestinal tissues, e.g., small intestinal duodenum, small intestinal jejunum, small intestinal ileum, large intestinal colon, etc., and between disease states of gastrointestinal tissue, i.e. healthy, pre-disease and diseased areas. Additionally, these microfluidic gut-on-chips allow identification of cells and cellular derived factors driving disease states and drug testing for reducing inflammation.

A method for aliquoting a sample liquid using a sealing liquid in a microfluidic device includes combining the sample liquid and the sealing liquid, which have different wetting behaviors, to form a two-phase system separated by a boundary surface. The microfluidic device includes a chamber with at least one inlet channel for introducing the liquids and a plurality of cavities configured to be filled via the inlet channel. The inlet channel and the cavities have a geometry that is defined in dependence on the respective wetting behaviors of the sample liquid and the sealing liquid. The method first includes introducing the sample liquid to form a first meniscus configured by the defined geometry, e.g. concave, to fill the cavities. The method further includes introducing the sealing liquid to form a second meniscus configured by the existing, greater contact angle and the defined geometry, e.g. convex, to cover the filled cavities.

A microfluidic device is useful for modelling drug transmission across the vasculature and vascular barriers. The device includes a frame, a fluid-permeable lumen configured to carry a fluid through the frame in a first direction, a first chamber surrounding the lumen, and a second chamber surrounding the first fluid-permeable chamber. At least one surface of the first chamber is configured for deposition of a first population of endothelial cells. An outer surface of the second chamber is configured for deposition a second population of cells. The second chamber is configured to carry a fluid through the frame in a second direction. The fluid-permeable lumen is configured to allow the fluid to permeate through a wall of the lumen into the first chamber, and the first chamber and the second chamber are in fluid communication with each other.

A microfluidic device comprising a channel within a substrate and a condenser or a hydrodynamic focusing chamber along the channel, configured to focus a fluid containing particles of a plurality of sizes. A negative angle deterministic lateral displacement (DLD) array is configured to receive the focused fluid and separate the particles in the focused fluid into three sizes ranges. The negative angle DLD array comprises a plurality of rows of pillars, wherein the rows of pillars are positioned to repeat a pattern every N rows with a shift of M columns, N and M are relatively coprime, and N is greater than 1.

A system for nucleic acid amplification is to synthesize amplified target nucleic acids or determine the presence of target nucleic acid. The mobile device of the system implements with an interface for controlling the reaction as well as optionally recording or delivering the reaction results or protocols to a cloud for sharing. In addition, current invention also discloses an airborne molecule detector integrating both air sampler and biochemical analysis component. The device can monitor the bioaerosols on real time. The reaction product can be used for nucleic acid sequencing as well. Furthermore, a pH test strip is used to replace a halochromic agent in a reaction mix for determining the nucleic acid amplification.

A method for filling microchambers with a chemistry in a substrate containing a plurality of microchambers comprises forming a channel in the substrate such that the channel is fluidically connected with the plurality of microchambers. The chemistry is delivered into the channel so that the chemistry is delivered to each of the microchambers. The chemistry is then permitted to incubate within each of the microchambers. Excess chemistry is then removed from the microchambers by introducing fluid through the channel to each of the connected microchambers.

A system for collecting a sample of a liquid, the system including a liquid storage vessel including an opening, and a capping element configured to seal the opening of the storage vessel is provided. The capping element includes a chamber configured to store a sample of the liquid separate to the liquid storage vessel. The capping element includes a pipette element defining the chamber that is configured to store the sample of liquid taken from the storage vessel.

A system configured to determine the load in a liquid sample of predetermined antigens is provided. The system comprises a measurement chamber configured for receipt therein of the liquid sample, a sensor circuit, and an analysis unit. The sensor circuit comprises a plurality of working electrodes, each comprising antibodies on its surface associated with one of the predetermined antigens, at least one reference electrode, and at least one counter electrode. Proximal ends of the electrodes are disposed on a reading zone of the sensor circuit, the reading zone being disposed within the measurement chamber. The analysis unit is configured to facilitate the determination of the load of each of the antigens by measuring electrical properties of the electrodes.

Dry reagent cup assemblies and methods are disclosed. In accordance with an implementation, an apparatus includes a liquid reservoir and dry reagent cup assembly. The liquid reservoir has a base, side wall that extends from the base, and distal opening. The dry reagent cup assembly coupled to the liquid reservoir includes a dry reagent cup and liquid impermeable barrier. The dry reagent cup has a cup base, cup side wall that extends from the cup base, and cup opening. The distal opening of the liquid reservoir faces the cup opening. The liquid impermeable barrier covers the cup opening and separates the liquid reservoir and the dry reagent cup. The dry reagent cup moves between an initial position outside the liquid reservoir and a rehydrating position where the dry reagent cup pierces and passes through an opening in the liquid impermeable barrier and is received within the liquid reservoir.