Examples include a fluid device. The fluid device includes a substrate, a sensor coupled on the substrate. A reservoir is formed in the substrate adjacent to the sensor. A deformable cover is disposed to seal the sensor and the reservoir on the substrate.

Devices, containers, and methods are provided for performing biological analysis in a closed environment. Illustrative biological analyses include nucleic acid amplification and detection and immuno-PCR.

Provided are a container with which the outflow rate of a fluid can easily be controlled, and which can be caused to function as part of a configuration of a diaphragm pump, as well as a microfluidic device and a diaphragm pump. The container includes a case body 110 that includes a tubular portion in which a fluid is to be sealed, a thin film 200 that closes an opening on one end side of the tubular portion and that is to be punctured so as to form an outflow port for the fluid R therein, and a diaphragm 300 that closes an opening on the other end side of the tubular portion.

The present invention provides system and methods for detecting an analyte indicative of an influenza viral infection in a sample of bodily fluid. The present invention also provides for systems and method for detection a plurality of analytes, at least two of which are indicative of an influenza viral infection in a sample of bodily fluid.

A cartridge comprises a chip chamber configured to house a microfluidic chip comprising a plurality of sets of cell channels configured to capture cells from a biological sample. The cartridge also comprises a sample chamber configured to receive the biological sample and be in fluid connection with the plurality of sets of cell channels and a plurality of medium reservoirs. Each medium reservoir of the plurality of medium reservoirs is configured to be in fluid connection with a respective set of cell channels of the plurality of sets of cell channels. The cartridge further comprises culture medium source in fluid connection with the plurality of medium reservoirs and configured to supply a culture medium to the plurality of medium reservoirs.

The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

The present invention is directed to the fabrication and use of phase-change material (PCM) membranes in microvalves for microfluidic systems. The microvalve may be fabricated by using a tissue-sectioning instrument to slice a thin membrane of PCM off of a block of PCM. The membrane may then be sandwiched between a plurality of microfluidic flow sections to act as a microvalve. At room temperature, the membrane may exist in a solid state to act as a zero-leakage seal and microvalve. Applying heat to the membrane may bring the membrane to a melting point, causing it to reach a liquid state. The microvalve in the liquid state may experience a surface tension effect by a material of the microfluidic flow sections, causing it to displace from a flow path and allow a fluid to pass from one microfluidic flow section to another.

Engineered or non-naturally occurring systems and compositions comprising a novel Cas12b and a guide molecule. Also provided include methods of use the systems and compositions, including in treating and diagnosing diseases.

An integrated molecular diagnosis apparatus including a buffer tube into which a sample collection tool collecting a sample is inserted, the buffer preparing a sample solution that contains nucleic acid extracted from the collected sample, a cartridge combined with the buffer tube and supplied with the sample solution, the cartridge transporting the sample solution to a reaction chamber through a fluid channel and performing a nucleic acid amplification reaction, and a diagnosis module main body detachably combined with the cartridge, the diagnosis module main body supplying heat at a predetermined temperature to the reaction chamber, detecting the nucleic acid amplification reaction, and determining whether or not a diagnosis target is present.

The disclosure relates to devices, solutions and methods for collecting and processing samples of bodily fluids containing cells (as well as embodiments for the collection, and processing and/or analysis of other fluids including toxic and/or hazardous substances/fluids). In addition, the disclosure relates generally to function genomic studies and to the isolation and preservation of cells from saliva and other bodily fluids (e.g., urine), for cellular analysis. With respect to devices for collection of bodily fluids, some embodiments include two mating bodies, a cap and a tube (for example), where, in some embodiments, the cap includes a closed interior space for holding a sample preservative solution and mates with the tube to constitute the (closed) sample collection device. Upon mating, the preservation solution flows into the closed interior space to preserve cells in the bodily fluid. The tube is configured to receive a donor sample of bodily fluid (e.g., saliva, urine), which can then be subjected to processing to extract a plurality of cells. The plurality of cells can be further processed to isolate one and/or another cell type therefrom. The plurality of cells, as well as the isolated cell type(s), can be analyzed for functional genomic and epigenetic studies, as well as biomarker discovery.