A microfluidic device for detection of an analyte in a fluid is described. The microfluidic device comprises a substrate having a first surface defining entrances to one or more chambers defined in the substrate, surfaces of the chambers defining a second surface of the substrate, the first surface being modified for selective targeting and capture of at least one analyte to operably effect a blocking of the entrance to at least one of the chambers, and wherein a response characteristic of the microfluidic device is operably varied by the blocking of the entrance to the at least one of the chambers, thereby providing an indication of the presence of the analyte within the fluid.

A sample holder (10) comprises a sample chamber (33), a gas reservoir (32) and an upper layer (20) covering over the sample chamber (33) and gas reservoir (32), wherein a bottom surface of the upper layer (20) comprises a microstructure array (23) which overlies at least a portion of a top periphery of the sample chamber (33), and wherein the microstructure array (23) is in communication with a gas path which extends to the gas reservoir (32), to allow gas exchange between the sample chamber (33) and the gas reservoir (32).

Methods and apparatus for controlling flow in a microfluidic arrangement are disclosed. In one arrangement, a microfluidic arrangement comprises a first liquid held predominantly by surface tension in a shape defining a microfluidic pattern on a surface of a substrate. The microfluidic pattern comprises at least an elongate conduit and a first reservoir. A second liquid is in direct contact with the first liquid and covers the microfluidic pattern. A flow of liquid is driven through the elongate conduit into the first reservoir. The microfluidic pattern and the depth and density of the second liquid are such that the first reservoir grows in volume during the flow of liquid into the first reservoir, without either of the size and shape of an area of contact between the first reservoir and the substrate changing, until an upper portion of the first reservoir detaches from a lower portion of the first reservoir due to buoyancy and rises upwards through the second liquid, thereby allowing the first reservoir to continue to receive liquid from the flow of liquid without any change in the size and shape of the area of contact between the first reservoir and the substrate.

The present disclosure provides a microfluidic device (1) comprising at least one flow channel (21) connecting an inlet opening (22) with an outlet opening (23), and an array of wells (24) in fluid communication with the flow channel (21), wherein the flow channel (21) comprises an inlet portion (211) at the inlet opening (22), an outlet portion (212) at the outlet opening (23), and a middle portion (213) between the inlet portion (211) and the outlet portion (212), wherein at least the middle portion (213) comprises the array of wells (24) and is provided with a hydrophilic surface, and the flow channel (21) further comprises a transition area (214) provided at least between the middle portion (213) and the outlet portion (212), which transition area (214) is constituted by providing a hydrophobic surface. Furthermore, a method for manufacturing such a microfluidic device is also provided.

A method for analyzing biological samples is disclosed herein. In an embodiment, the method includes receiving a fluid sample into a cartridge device, which comprises: a fluidic chamber; at least one microfluidic channel in fluid communication with the fluidic chamber; and a venting port configured to apply a pneumatic force to the fluidic chamber; and inserting the cartridge device into a reader device to perform measurements, wherein the cartridge device is positioned in a vertical or tilted position such that at least a portion of the fluid sample inside the fluidic chamber is pulled by gravity in a direction away from the venting port or towards the bottom of the fluidic chamber.

An open microfluidic system is provided. The open microfluidic system including the extreme wettability of exclusive liquid repellency (ELR), open microchannels with high lateral resolution and low profile, various valve arrangements, capable of a broad range flow rates, and capable of spatially and temporally trapping particles in open fluid.

Various aspects of the present disclosure are directed toward methods and apparatuses for interacting a first liquid and a second liquid in one or more fluidic channels of a capillary structure. The methods and apparatuses can include providing at least one capillary barrier that positions a meniscus of the first liquid at a fluid-interface region using capillary forces within the capillary structure. Additionally, a path is provided along one of the channels for the second liquid to flow toward the fluid-interface region. Additionally, gas pressure is released, via a gas-outflow port, from the fluid-interface region while flow of the first liquid is arrested. Further, the first liquid and the second liquid contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region.

The disclosure relates to devices and methods for analyzing particles in a sample. In various embodiments, the present disclosure provides devices and methods for cytometry and additional analysis. In various embodiments, the present disclosure provides a cartridge device and a reader instrument device, wherein the reader instrument device receives, operates, and/or actuates the cartridge device. In various embodiments, the present disclosure provides a method of using a device as disclosed herein for analyzing particles in a sample.

A centrifugal rotor device includes a first chamber configured to hold a fluid, and a second chamber configured to receive the fluid from the first chamber. The centrifugal rotor device also includes a conduit coupled to the first chamber at a conduit inlet and coupled to the second chamber at a conduit outlet, the conduit configured to permit movement of the fluid from the first chamber to the second chamber. The conduit includes a first channel and a second channel formed adjacent to the first channel. The second channel is in fluid communication with the first channel and has a dimension smaller than the smallest dimension of the first channel. The conduit also includes one or more obstructive features present in the second channel configured to impede movement of the fluid in the second channel.

The present disclosure relates to an engineered, additively manufactured, microfluidic cellular structure formed from a plurality of cells, wherein the cells are each formed from a plurality of interconnected elements. The cells have voids and each cell is open at upper ends thereof. The cells each communicate at a point below its upper end with a common channel. The cells are each configured to accept a fluid and operate to channel the fluid into the common channel and to hold the fluid received therein for later selective withdrawal from the structure.