The present invention provides a new and distinct cannabis cultivar designated as HOLY CRUNCH.

The present invention provides a new and distinct cannabis cultivar designated as RAINBOW GUMMEEZ.

The present invention provides a new and distinct cannabis cultivar designated as GUAVA JAM.

The present invention provides a new and distinct cannabis cultivar designated as GRAPE LOLIPOP.

The present invention provides a new and distinct cannabis cultivar designated as RASPBERRY PUNCH.

Compositions and methods for reducing the level of nornicotine and N-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Toxin-free mutant aloe has attenuated expression of at least one gene involved in the biosynthesis of a natural toxin, such as aloin. Methods of producing toxin-free mutant aloe involve obtaining plant cells derived from a species of aloe and attenuating the expression level of at least one polyketide synthase gene within the obtained plant cells to form mutant plant cells. Methods further involve culturing the mutant plant cells under a set of culture conditions conducive to proliferation of the mutant plant cells. Methods further involve growing the mutant plant cells in a bioreactor and harvesting a toxin-free aloe bioproduct therefrom. Attenuating the expression level of at least one polyketide synthase gene involves disrupting the gene with a targeted endonuclease system and selecting the successfully mutated cells prior to cell culturing and expansion.

According to the invention, there is provided null-LOX-1 barley and plant products produced thereof, such as malt manufactured by using barley kernels defective in synthesis of the fatty acid-converting enzyme lipoxygenase-1. Said enzyme accounts for the principal activity related to conversion of linoleic acid into 9-hydroperoxy octadecadienoic acid, a lipoxygenase pathway metabolite, whichthrough further enzymatic or spontaneous reactionsmay lead to the appearance of trans-2-nonenal. The invention enables brewers to produce a beer devoid of detectable trans-2-nonenal-specific off-flavors, even after prolonged storage of the beverage.

Compositions and methods for reducing the level of nornicotine and N-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Compositions and methods for reducing the level of nornicotine and N-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.