The present invention provides a wheat plant comprising an Rht-B1 allele which encodes an Rht-B1 (DELLA) poly-peptide. Grain from a near-isogenic wheat line comprising the dwarfing Rht-B1c allele was subjected to sodium azide mutagenesis. Plants exhibiting early leaf elongation rates or mature plant height greater than the dwarf parent were selected and the Rht-B1 gene sequenced. This identified 35 mutated alleles of Rht-B1c. Similar methods were also used to identify mutant alleles of the dwarfing s1n1d allele in barley, where DELLA is encoded by the s1n1 gene.

Methods are described for selecting or producing a cereal plant comprising a functional restorer gene for wheat G-type cytoplasmic male sterility and nucleic acids and/or polypeptides for use therein.

The present disclosure relates to wheat plants having grain with high β-glucan content, methods for constructing said wheat plants, grain therefrom and uses thereof. Wheat lines homozygous for the HvCslF6 gene from barley having five times the fiber content and an increased β-glucan content of TA5790, TA5792, and TA5795 are disclosed.

In one aspect, this disclosure relates to a wheat plant, plant part or plant cell that has increased resistance to powdery mildew, wherein said plant comprises a loss of function mutation in the coding regions of two alleles selected from TaMLO-A1, TaMLO-B1 and TaMLO-D1 and reduced expression of the third TaMLO allele. In another aspect, this disclosure provides a method for producing a wheat plant, plant part or plant cell with increased resistance to powdery mildew, wherein the method comprises using targeted genome modification comprising introducing a loss of function mutation into the coding regions of two MLO alleles selected from TaMLO-A1, TaMLO-B1 and TaMLO-D1 and decreasing expression of the third TaMLO allele.

The present invention provides a wheat plant comprising an Rht-B1 allele which encodes an Rht-B1 (DELLA) polypeptide. Grain from a near-isogenic wheat line comprising the dwarfing Rht-B1c allele was subjected to sodium azide mutagenesis. Plants exhibiting early leaf elongation rates or mature plant height greater than the dwarf parent were selected and the Rht-B1 gene sequenced. This identified 35 mutated alleles of Rht-B1c. Similar methods were also used to identify mutant alleles of the dwarfing sln1d allele in barley, where DELLA is encoded by the sln1 gene.

Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part. In certain embodiments, the plant is polyploid.

A series of independent human-induced non-transgenic mutations found at one or more of the SBEII genes of wheat; wheat plants having these mutations in one or more of their SBEII genes; and a method of creating and finding similar and/or additional mutations of SBEII by screening pooled and/or individual wheat plants. The seeds and flour from the wheat plants of the present invention exhibit an increase in amylose and resistant starch without having the inclusion of foreign nucleic acids in their genomes. Additionally, the wheat plants of the present invention exhibit altered SBEII activity without having the inclusion of foreign nucleic acids in their genomes.

This invention describes a new method to generate wheat seed. The process involves the delivery of pollen of the male parent at will, as available either in a preserved pollen bank, or using real-time collection from male plants as they become available. Desired pollen is delivered to fertile females during the period when viable pollen from the females and locally proximal unrelated plants is not being released. The delivered male pollen is in such amounts and fortuitously timed that it preferentially pollinates the females. Such fortuitous timing may involve the intentional application of pollen to females a day or two prior to female parent pollen becoming viable, and/or during several periods wherein female parent pollen and/or other proximal plant pollen is not being shed.

High throughput methods are described for identifying combinations of mutations that can be used to improve a phenotypic feature in an organism. Large populations of organisms (e.g., plants) containing different combinations of mutations can be assessed using the methods.

The present invention provides wheat grain of the species Triticum aestivum, the grain comprising i) mutations in each of its SSIIa genes such that the grain is homozygous for a mutation in its SSIIa-A gene, homozygous for a mutation in its SSIIa-B gene and homozygous for a mutation in its SSIIa-D gene, wherein at least two of the mutations in said SSIIa genes are null mutations, mutations in each of its SSIIIa genes such that the grain is homozygous for a mutation in its SSIIIa-A gene, homozygous for a mutation in its SSIIIa-B gene and homozygous for a mutation in its SSIIIa-D gene, wherein at least two of the mutations in said SSIIIa genes are null mutations ii) a total starch content comprising an amylose content and an amylopectin content, iii) a fructan content which is increased relative to wild-type wheat grain on a weight basis, preferably between 3% and 12% of the grain weight, iv) a -glucan content, v) an arabinoxylan content, vi) a cellulose content.
The grain has a weight of between 25 mg and 60 mg, and the amylose content is between 45% and 70% on a weight basis of the total starch content of the grain as determined by iodine binding assay. The amylopectin content on a weight basis is reduced relative to the wild-type wheat grain, and each of the -glucan content, arabinoxylan content and cellulose content are increased relative to the wild-type wheat grain on a weight basis, such that the sum of the fructan content, -glucan content, arabinoxylan content and cellulose content is between 15% and 30% of the grain weight.