Disclosed is a method for determining conditions for cultivating a plant body expressing a protein of interest or a peptide of interest in a 24-hour cycle in which a dark period and a light period are repeated alternately, including: a step of determining an optimum leaf temperature based on a capability to express a protein of interest or a peptide of interest; and a step of setting light and dark period leaf temperatures and light and dark period time lengths after a plant body is infected with a bacterium or a virus having a polynucleotide encoding the protein of interest or the peptide of interest so as to satisfy the following formula, the following formula being: Dark period leaf temperature ( C.)Dark period time length (hour)+Light period leaf temperature ( C.)Light period time length (hour)=Optimum leaf temperature ( C.)24 (hour)24.

The invention relates to the field of tobacco breeding, particularly to a tobacco plant resistant to TSWD without linkage drag and a method for breeding the same. Provided is a tobacco plant or germplasm resistant to TSWD, which comprises a short RTSW introgressed segment, wherein at least part or entire of the sequence set forth in SEQ ID No. 34 is deleted in the short RTSW introgressed segment as compared to the RTSW introgressed segment of tobacco Polata. Also provided is a method for screening said tobacco plant or germplasm, in which a tobacco plant or germplasm resistant to TSWD carrying a short RTSW introgressed segment is obtained by detecting NaChr4_2M, NaChr4_8M, NaChr3_62.6M and NaChr3_64.6M linkage drag locus markers. Compared with Polalta, the tobacco plant or germplasm provided by the invention not only has TSWD resistance, but also reduces or removes linkage drag.

The present disclosure provides tobacco inbred plants TND950 (phph) SRC, Narrow Leaf Madole (phph) SRC, Narrow Leaf Madole SRC, CMS TND950 SRC, CMS Narrow Leaf Madole SRC, and CMS KY171 SRC, and hybrids PD7305 SRC, PD7309 SRC, PD7312 SRC, PD7318 SRC, and PD7319 SRC. The present disclosure also provides parts of such plants and products made from those parts. The present disclosure also includes progeny of the provided plants including hybrids.

The invention relates to the isolation and the cloning and breeding application of a tobacco mosaic virus (TMV) resistant Nau gene. The invention discloses the nucleotide sequence of the TMV resistant Nau gene shown as SEQ ID NO.1. The amino acids of polypeptide encoded by the TMV resistant Nau gene are shown as SEQ ID NO.2. A non-transgenic TMV resistant tobacco variety can be obtained by transferring an Nau gene which is contained by germplasm resources into a TMV infected popular tobacco variety by conventional breeding means. The Nau gene of the invention has a homologous sequence with high identity rate of nucleotides in the popular tobacco variety, so that it is easy to obtain a shorter introgression segment single plant carrying the Nau gene by conventional breeding, and thereby to obtain a TMV resistant variety with lower linkage drag. The gene can resist both U1 strain and Cg strain of TMV. The novel antiviral gene Nau of the invention has great application prospect in cultivation of a TMV resistant tobacco.

The present disclosure provides the identification of tobacco Nic1 locus. Also provided are tobacco plants with altered total alkaloid and nicotine levels and commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants. Also provided are compositions and methods for producing tobacco plants having novel Nic1 mutations or alleles to reduce nicotine levels. Further provided are sequence polymorphisms and molecular markers for breeding tobacco with reduced nicotine or nicotine free while maintaining tobacco leaf grade and tobacco product quality.

The present invention generally relates to methods and materials involved in producing tobacco plants having reduced levels of conversion of nicotine to nornicotine. In certain embodiments, the invention is directed to mutations in a nicotine demethylase gene, tobacco plants comprising mutations in a nicotine demethylase gene, and tobacco compositions and products thereof. In other embodiments, the invention is directed toward nicotine demethylase RNA interference, tobacco plants comprising a nicotine demethylase RNA interference transgene, and tobacco compositions and products thereof.

The present disclosure provides composition, systems, devices and methods for plant-based production of non-plant proteins through the use of heterologous genes for the expression of said non-plant proteins. Non-plant proteins can include, but are not limited to, mammalian proteins, cytokines, or growth factors.

Compositions and methods for reducing the level of nornicotine and N-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided. The methods comprise introducing into the genome of a tobacco plant a mutation within at least one allele of each of at least three nicotine demethylase genes, wherein the mutation reduces expression of the nicotine demethylase gene, and wherein a first of these nicotine demethylase genes encodes a root-specific nicotine demethylase involved in the metabolic conversion of nicotine to nornicotine in a tobacco plant or a plant part thereof. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

An object of the present invention is to provide an F1 hybrid or a portion thereof, and use of the F1 hybrid or a portion thereof. The F1 hybrid of the present invention is an F1 hybrid between Nicotiana umbratica and Nicotiana kawakamii.

Provided herein are genetic markers and a coding sequence associated with a low- or ultra-low anatabine trait in tobacco.