Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

Methods, assays, compositions and kits for the ligation of short polynucleotides are presented herein. The short polynucleotides are optionally no more than 7 nucleotides in length, and can be as short as 3 or 4 nucleotides in length. The ligation is optionally performed by CV ligase.

A method of producing a packaging structure, including the steps: coating at least one surface of a first sheet base material and/or a second sheet base material with a synthetic polyisoprene latex having a weight average molecular weight of 500,000 -5,000,000 and/or a styrene-isoprene-styrene block copolymer having a weight average molecular weight of 100,000-300,000, sandwiching an article to be packaged in a state wherein the first and second sheet base material are in contact with each other via a latex coated surface formed on the first and/or second sheet base material to thereby obtain a laminated body, pressing a portion where at least the first and second sheet base material of the laminated body are in contact with each other via the latex coated surface at a temperature of 100° C. or less to thereby obtain a pressed laminated body, and performing a sterilization treatment on the pressed laminated body.

A method of producing a packaging structure, including the steps: coating at least one surface of a first sheet base material and/or a second sheet base material with a synthetic polyisoprene latex having a weight average molecular weight of 500,000 -5,000,000 and/or a styrene-isoprene-styrene block copolymer having a weight average molecular weight of 100,000-300,000, sandwiching an article to be packaged in a state wherein the first and second sheet base material are in contact with each other via a latex coated surface formed on the first and/or second sheet base material to thereby obtain a laminated body, pressing a portion where at least the first and second sheet base material of the laminated body are in contact with each other via the latex coated surface at a temperature of 100° C. or less to thereby obtain a pressed laminated body, and performing a sterilization treatment on the pressed laminated body.

The present disclosure provides a system and method for smart auto-vending during a pandemic. The purpose is to increase efficiency in order fulfillment of prepacked and non-prepacked products in terms of speed, low cost, and sterilization. The system comprises: a box station; dispensers/dispensing subsystems; sterilization stations with different sterilization methods; a packaging station with different packaging methods; a shipping station for smart shipping with automatic self-driving vehicles or drones; a main conveyor and sub-conveyors that move an order through the system. In addition, there may be one dispensing subsystem for weighing, bagging, and sterilizing non-prepackaged grocery products. The system's AI function is also used to optimize items stored in each dispenser/dispensing subsystem. All of these components and methods come together to form an integrated, streamlined, and optimized system and method for carrying out online order fulfillment efficiently.

To make a sterilization step appropriate in an aseptic filling apparatus. Provided is an aseptic filling apparatus including a step of sterilizing a preform and a step of sterilizing a container obtained by molding the preform, wherein assuming sterilization effects in the respective sterilization steps as X [LRV] and Y [LRV], a relationship of 5≤X+Y≤10 (where Y≥0) is established.

The present disclosure provides novel processes for providing sterile, pre-filled syringes or injection devices for pharmaceutical compositions, including protein and biopharmaceutical formulations. In some examples, secondary packaging is performed in an aseptic environment, which removes the need for any terminal sterilization method. In some examples, the aseptic packaging method results in a sterile injection device suitable for use in ophthalmic injections.

A method is described of filling and/or sealing a packaging container. The packaging container can include a packaging laminate and having a top portion and a lower portion including one or more side walls and an open base. The method can include: supporting the packaging container with the open base uppermost on a support with an upstanding portion, wherein the support engages the top portion of the packaging container and engages part of the one or more side walls via the upstanding portion of the support; and filling the packaging container via its open base and/or sealing the open base of the packaging container, wherein the support ensures dimensional stability of the packaging container during the filling and/or sealing step. A related apparatus, support and packaging container are also described.

The invention relates to a device comprising a first fixing element, a further fixing element and a folded planar composite; wherein the first fixing element comprises a first fixing surface and the further fixing element comprises a further fixing surface; wherein the folded planar composite is at least partially fixed between the first fixing surface and the further fixing surface; wherein the folded planar composite comprises a first composite region; wherein the first composite region comprises a first layer sequence comprising a first composite layer comprising a first carrier layer, a second composite layer comprising a second carrier layer, a third composite layer comprising a third carrier layer and a fourth composite layer comprising a fourth carrier layer; wherein in the first composite region the second composite layer is joined to the third composite layer and the third composite layer is joined to the fourth composite layer; wherein in the first composite region the third carrier layer is characterised by a smaller layer thickness than in each case one selected from the group consisting of the first carrier layer, the second carrier layer and the fourth carrier layer or a combination of at least two of these; wherein the first fixing surface or the further fixing surface or both comprises a recess comprising a first recess region; wherein the first composite region is located at least partially between the first recess region and the first fixing surface or the further fixing surface.

The invention relates to a device comprising a first fixing element, a further fixing element and a folded planar composite; wherein the first fixing element comprises a first fixing surface and the further fixing element comprises a further fixing surface; wherein the folded planar composite is at least partially fixed between the first fixing surface and the further fixing surface; wherein the folded planar composite comprises a first composite region; wherein the first composite region comprises a first layer sequence comprising a first composite layer comprising a first carrier layer, a second composite layer comprising a second carrier layer, a third composite layer comprising a third carrier layer and a fourth composite layer comprising a fourth carrier layer; wherein in the first composite region the second composite layer is joined to the third composite layer and the third composite layer is joined to the fourth composite layer; wherein in the first composite region the third carrier layer is characterised by a smaller layer thickness than in each case one selected from the group consisting of the first carrier layer, the second carrier layer and the fourth carrier layer or a combination of at least two of these; wherein the first fixing surface or the further fixing surface or both comprises a recess comprising a first recess region; wherein the first composite region is located at least partially between the first recess region and the first fixing surface or the further fixing surface.