Provided is a method for artificial rearing of porcupine pufferfish fries in indoor cement ponds. The method includes the following steps: indoor cement pond preparation, release hatched larvae into the indoor cement pond, feed and manage the larvae at different sub-stages of the fry rearing stages, water quality management during fry rearing, separate ponding of fries based on size of the fries, and transfer the fries to net cages or outdoor ponds when the fries reach a minimum length. The indoor cement pond preparation step may include disinfecting the pond and regulating the water quality. During the rearing stage, the method may include stimulating anti-stress physiological response of the larvae. The method may transfer fries with an average total length of more than 2.0 cm into net cages or outdoor ponds for aquaculture after 45 days of the rearing stage.

This invention relates to a system for detecting and retrieving selected specimens being at least one of fish eggs, roe or fry, from a water containing hatching tray. The system including retrieving unit including a tube having a width comparable to the size of the specimens to be retrieved, the tube end being configured to be position in the water, and a pump device cable of pumping water and specimens from said tray. It also includes a robot unit moving the retrieving unit in a predetermined path related to a first reference system over said tray. The system also includes an imaging device being configured to detect and select specimens having chosen characteristics, and a control unit defining a second reference system defined by the position of a selected specimen relative to the position of the retrieving unit, the system being configured to move the tube end to the position of the selected specimen and pumping the specimen into the tube.

This invention relates to a system for detecting and retrieving selected specimens being at least one of fish eggs, roe or fry, from a water containing hatching tray. The system including retrieving unit including a tube having a width comparable to the size of the specimens to be retrieved, the tube end being configured to be position in the water, and a pump device cable of pumping water and specimens from said tray. It also includes a robot unit moving the retrieving unit in a predetermined path related to a first reference system over said tray. The system also includes an imaging device being configured to detect and select specimens having chosen characteristics, and a control unit defining a second reference system defined by the position of a selected specimen relative to the position of the retrieving unit, the system being configured to move the tube end to the position of the selected specimen and pumping the specimen into the tube.

Breeding system (1) for fish belonging to the Cyprinidae family, and more particularly for zebra fish of the species Danio rerio, the said system comprising a breeding tank (2) having a bottom (2a) and side walls (2b), a spawning support (3) placed in the breeding tank (2) and comprising a platform (4) forming a spawning zone positioned a distance away from the bottom (2a) of the breeding tank (2), and a water circulating system (17) configured to modify the water level in the breeding tank (2) and at least partially flood the platform (4) of the spawning support (3), in which breeding system the spawning support (3) comprises a funnel (7) comprising a truncated conical wall (8) having a lower orifice (9) extending near the bottom (2a) of the breeding tank (2) and in fluidic communication with the water circulating system (17) by means of a pipe (11), and an upper orifice (10) extending some distance away from the bottom (2a), the platform (4) being fixed near the upper orifice (10) of the funnel (7), and the breeding system further comprises a removable shut-off member (13) configured to at least partially block off the pipe (11) of the spawning support (3) so as to increase the water level in the breeding tank (2) so as to at least partially flood the platform (4), the shut-off member (13) comprising a pipe at the end of which through-holes are formed, the said pipe having a height that is greater than a distance between the lower orifice (9) and the upper orifice (10).

Breeding system (1) for fish belonging to the Cyprinidae family, and more particularly for zebra fish of the species Danio rerio, the said system comprising a breeding tank (2) having a bottom (2a) and side walls (2b), a spawning support (3) placed in the breeding tank (2) and comprising a platform (4) forming a spawning zone positioned a distance away from the bottom (2a) of the breeding tank (2), and a water circulating system (17) configured to modify the water level in the breeding tank (2) and at least partially flood the platform (4) of the spawning support (3), in which breeding system the spawning support (3) comprises a funnel (7) comprising a truncated conical wall (8) having a lower orifice (9) extending near the bottom (2a) of the breeding tank (2) and in fluidic communication with the water circulating system (17) by means of a pipe (11), and an upper orifice (10) extending some distance away from the bottom (2a), the platform (4) being fixed near the upper orifice (10) of the funnel (7), and the breeding system further comprises a removable shut-off member (13) configured to at least partially block off the pipe (11) of the spawning support (3) so as to increase the water level in the breeding tank (2) so as to at least partially flood the platform (4), the shut-off member (13) comprising a pipe at the end of which through-holes are formed, the said pipe having a height that is greater than a distance between the lower orifice (9) and the upper orifice (10).

Described herein are polymorphisms, and in particular single nucleotide polymorphisms (SNP) associated with late onset of sexual maturation in rainbow trout (Oncorhynchus mykiss). In particular, provided are methods for predicting late onset of sexual maturation in rainbow trout, methods for selecting a rainbow trout having late onset of sexual maturation and kit suitable for carrying out said methods. Further provided are rainbow trout cells, sperm and unfertilized eggs carrying at least one allele conferring late onset of sexual maturation in their genome.

Described herein are polymorphisms, and in particular single nucleotide polymorphisms (SNP) associated with late onset of sexual maturation in rainbow trout (Oncorhynchus mykiss). In particular, provided are methods for predicting late onset of sexual maturation in rainbow trout, methods for selecting a rainbow trout having late onset of sexual maturation and kit suitable for carrying out said methods. Further provided are rainbow trout cells, sperm and unfertilized eggs carrying at least one allele conferring late onset of sexual maturation in their genome.

Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

A fish egg incubator including a housing containing a reciprocating mechanism, at least one connector assembly associated with the reciprocating mechanism, at least one perforated containing assembly for holding the fish eggs located at least partially below a water surface and which is associated with the connector assembly where in the reciprocating mechanism periodically moves the least one perforated containing assembly in the water in order to move the eggs in the at least one perforated containing assembly.