Transgenic silkworms stably expressing synthetic spider silk genes or composite silkworm/spider silk genes are disclosed. The exogenous spider silk genes are stably intergrated into a defined site of the fibroin heavy chain intron or a fibroin light chain intron of silkworms. Synthetic spider silk proteins and composite spider silk-silkworm genes and proteins are provided. The expression of exogenous spider silk genes is driven by the endogenous fibroin heavy chain promoter, improving the genetic stability of transgenic silkworms. The composite silkworm/spider silk fibers exhibit exceptional mechanical performance, compared to normal silkworm silk fibers and other transgenic silkworm fibers.

Transgenic silkworms stably expressing synthetic spider silk genes or composite silkworm/spider silk genes are disclosed. The exogenous spider silk genes are stably intergrated into a defined site of the fibroin heavy chain intron or a fibroin light chain intron of silkworms. Synthetic spider silk proteins and composite spider silk-silkworm genes and proteins are provided. The expression of exogenous spider silk genes is driven by the endogenous fibroin heavy chain promoter, improving the genetic stability of transgenic silkworms. The composite silkworm/spider silk fibers exhibit exceptional mechanical performance, compared to normal silkworm silk fibers and other transgenic silkworm fibers.

Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with the heavy chain fibroin protein. Embodiments include insertion and truncation vectors utilized for modifying the FibH gene. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into the FibH gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing an enhanced silk product with a high percentage of spider silk proteins. Embodiments include a silk product produced by such transgenic Bombyx mori.

Described herein are methods of producing transgenic Bombyx mori by targeting and modifying genomic regions associated with the heavy chain fibroin protein. Embodiments include insertion and truncation vectors utilized for modifying the FibH gene. Embodiments include plasmid constructs utilized for molecular cloning of donor sequences configured for replacement of or insertion into the FibH gene and utilized for transfection of Bombyx mori with the donor sequences. Embodiments include transgenic Bombyx mori that have been transfected with the donor sequences and are capable of producing an enhanced silk product with a high percentage of spider silk proteins. Embodiments include a silk product produced by such transgenic Bombyx mori.

Provided is a method developed for producing a thread bundle with high efficiency in terms of spun thread collection and without causing mechanical damage to a foothold silk thread spun by a bagworm onto a base material. After a bagworm is placed along with a solvent-soluble base material and allowed to spin the foothold silk thread onto the surface of the base material, the base material is dissolved with a solvent to separate the foothold silk thread therefrom.

Provided is a method developed for producing a thread bundle with high efficiency in terms of spun thread collection and without causing mechanical damage to a foothold silk thread spun by a bagworm onto a base material. After a bagworm is placed along with a solvent-soluble base material and allowed to spin the foothold silk thread onto the surface of the base material, the base material is dissolved with a solvent to separate the foothold silk thread therefrom.

Embodiments of the present disclosure include silk-based products and related methods of use in a variety of applications. Included are applications in the fields of medicine, veterinary medicine, agriculture, and material science.

An object of the present invention is to develop a method of producing pure and long bagworm silk threads having no contaminants, such as pieces of leaves and twigs, and a device for implementing the production method.

Provided is a method of producing a long bagworm silk thread, the method having a spinning process of making a bagworm having its nest hang its legs on a rail and continuously spin a silk thread along the rail, wherein the rail has a width smaller than the maximum width between the left and right legs when the bagworm used spreads out its legs and wherein the rail is such that the bagworm can hang its legs on the rail, and a collection process of collecting a long silk thread from the rail after the spinning process.

An object of the present invention is to develop a method of producing pure and long bagworm silk threads having no contaminants, such as pieces of leaves and twigs, and a device for implementing the production method.

Provided is a method of producing a long bagworm silk thread, the method having a spinning process of making a bagworm having its nest hang its legs on a rail and continuously spin a silk thread along the rail, wherein the rail has a width smaller than the maximum width between the left and right legs when the bagworm used spreads out its legs and wherein the rail is such that the bagworm can hang its legs on the rail, and a collection process of collecting a long silk thread from the rail after the spinning process.

Disclosed is a bombyx mori hatching and young silkworm rearing frame that includes a rearing frame main body (1) and a rearing frame drawer (2), wherein the size of the rearing frame is convenient for needs of silkworm rearing production; the rearing frame drawer (2) can be used for storing silkworm eggs during hatching and adding water to moisturize during rearing; the shape and size of grooves (4) of the rearing frame drawer (2) and those of meshes of a middle meshed interlayer (3) are both designed according to physiological needs of silkworm bodies; a stacked fixing device is further arranged at the periphery of the rearing frame; and a carbonate polymer layer is arranged on the surface of the meshed interlayer (3). According to the use of the rearing frame, the labor productivity of hatching and young silkworm rearing can be improved, and a novel rearing instrument can be provided for large-scale and labor-saving silkworm rearing.