The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning.

The invention encompasses methods of increasing the rate of genetic progress or generating inbred lines in a non-human mammalian species comprising the use of gametes derived from embryos of the non-human mammalian species.

The present invention relates to the production and culture of undifferentiated spermatogonial stem cells that can be maintained long term and are feeder free. The resultant feeder-free populations can be used in any of a number of protocols including the generation of progeny bulls. The present invention includes novel methods required for the successful enrichment of bovine spermatogonial stem cells, novel cell lines and other components used for the same, as well as the resultant stem cell compositions.

Systems for selecting, generating, and breeding hybrid dairy cattle are described, as are methods for maintaining herds of hybrid cattle.

The present specification relates to a transgenic animal and a transgenic embryo producing components of an engineered nuclease. According to the disclosure of the present specification, the transgenic animal (or embryo) producing components of an engineered nuclease is a transgenic animal (or embryo) which includes a first cell having a genome including a first toolbox; and a second cell having a genome including a second toolbox, wherein the first toolbox and the second toolbox include at least one of a polynucleotide encoding an RNA-guided endonuclease and a polynucleotide encoding a guide nucleic acid that is able to specifically bind to a target site, respectively, wherein the first toolbox is present in a first locus of the genome of the first cell; the second toolbox is present in a second locus of the genome of the second cell; and the first locus is different from the second locus.

Systems for selecting, generating, and breeding hybrid dairy cattle are described, as are methods for maintaining herds of hybrid cattle.

Systems for selecting, generating, and breeding hybrid dairy cattle are described, as are methods for maintaining herds of hybrid cattle.

The invention includes a system and method for evaluating the quality of pen rider activities in an environment such as a feedlot. The quality of the activities relate to individual rides conducted by pen riders in a feedlot in which the rides are numerically scored. The score is a function of a plurality of measured factors that specifically correspond to the physical actions of a pen rider while occupying a pen. The measured factors may be incorporated within an algorithm for determining the numerical score. The measured factors may include the number of seconds paused within a pen, a ride length within the pen, a ride speed within the pen, the number of rotations of the rider within the pen, and combinations thereof. The quality of activities is also evaluated by visual records showing routes taken as recorded by GPS locations. User interfaces are automatically generated to show ride scores and routes taken by riders on a graphical display.

Systems for selecting, generating, and breeding hybrid dairy cattle are described, as are methods for maintaining herds of hybrid cattle.

The present disclosure relates to methods for making genetic edits in vitro in a non-human vertebrate cell or embryo at a plurality of target chromosomal DNA sites. Methods for making a non-human animal having multiplex genetic edits at a plurality of target chromosomal DNA sites and making a non-human vertebrate animal chimeric for host cells and donor cells are also considered.