The invention relates to a device for use in large-scale industrial insect farming. More in particular, the invention relates to an insects transport device for transporting live insects from a first location to a predetermined second location, the insects transport device comprising a gas guiding unit, a gas discharge member and a feeder arrangement, wherein the insects transport device is configured to receive live insects such as freshly hatched neonate larvae, for example of black soldier fly, or mites, wherein the live insects are directly taken up in a laminar flow of gas after exiting the feeder arrangement in a free fall under influence of gravitation such that the live insects do not contact any surface of the insects transport device, and while in said gas are transported to a predetermined location in the insects transport device. Furthermore, the invention relates to the use of the device in industrial insect farming, such as large-scale farming of black soldier flies or mites. The invention also relates to a method of dosing live insects, wherein for example live insects are dosed which are essentially of the same age (e.g. within an age difference of 1 second-5 minutes), such as freshly hatched neonate larvae. In addition, the invention relates to a single dose of neonate larvae obtained with the method, wherein larvae have a small larvae-to-larvae age difference within the single dose.

Disclosed herein are devices, systems, and methods for measuring an electropharyngeogram (EPG) in living organisms. In some embodiments, the devices and systems disclosed herein include an array for sorting and immobilizing an organism (such as a nematode) for measurement of an EPG and/or optical imaging. Also disclosed are methods for identifying therapeutic or toxic compounds utilizing the disclosed devices and systems. In some embodiments, the methods include screening for compounds with anthelmintic activity, toxicity (for example HERG channel blockers), or candidate drugs for treatment of a variety of human and/or animal diseases.

Disclosed herein are devices, systems, and methods for measuring an electropharyngeogram (EPG) in living organisms. In some embodiments, the devices and systems disclosed herein include an array for sorting and immobilizing an organism (such as a nematode) for measurement of an EPG and/or optical imaging. Also disclosed are methods for identifying therapeutic or toxic compounds utilizing the disclosed devices and systems. In some embodiments, the methods include screening for compounds with anthelmintic activity, toxicity (for example HERG channel blockers), or candidate drugs for treatment of a variety of human and/or animal diseases.

The present invention relates to the field of biotechnology, in particular to genes and use thereof. The present invention employs whole genome sequencing to perform whole genome re-sequencing on a large number of individuals of the honey bee Apis mellifera sinisxinyuan, and obtains genes specific to the A. m. sinisxinyuan. The genes play important roles in the differentiation of A. m. sinisxinyuan from the honey bees in other regions and in the adaptive evolution of A. m. sinisxinyuan to the local environment. The Foxo gene or the Ebony gene provided in the present invention can be used to identify A. m. sinisxinyuan from other subspecies; can also be used for studying the genetic diversity of species resources of bees; and can further be used for studying cold resistance genes. This will fill the gap in the research field of A. m. sinisxinyuan by Chinese researchers.

The present invention relates to the field of biotechnology, in particular to genes and use thereof. The present invention employs whole genome sequencing to perform whole genome re-sequencing on a large number of individuals of the honey bee Apis mellifera sinisxinyuan, and obtains genes specific to the A. m. sinisxinyuan. The genes play important roles in the differentiation of A. m. sinisxinyuan from the honey bees in other regions and in the adaptive evolution of A. m. sinisxinyuan to the local environment. The Foxo gene or the Ebony gene provided in the present invention can be used to identify A. m. sinisxinyuan from other subspecies; can also be used for studying the genetic diversity of species resources of bees; and can further be used for studying cold resistance genes. This will fill the gap in the research field of A. m. sinisxinyuan by Chinese researchers.

The present invention relates to the field of biotechnology, in particular to genes and use thereof. The present invention employs whole genome sequencing to perform whole genome re-sequencing on a large number of individuals of the honey bee Apis mellifera sinisxinyuan, and obtains genes specific to A. m. sinisxinyuan. The genes play important roles in the differentiation of A. m. sinisxinyuan from the honey bees in other regions and in the adaptive evolution of A. m. sinisxinyuan to the local environment. The FilI gene or the Ds gene provided in the present invention can be used to identify A. m. sinisxinyuan from other subspecies; can also be used for studying the genetic diversity of species resources of bees; and can further be used for studying stress resistance genes. This will fill the gap in the research field of A. m. sinisxinyuan by Chinese researchers.

The present invention relates to the field of biotechnology, in particular to genes and use thereof. The present invention employs whole genome sequencing to perform whole genome re-sequencing on a large number of individuals of the honey bee Apis mellifera sinisxinyuan, and obtains genes specific to A. m. sinisxinyuan. The genes play important roles in the differentiation of A. m. sinisxinyuan from the honey bees in other regions and in the adaptive evolution of A. m. sinisxinyuan to the local environment. The FilI gene or the Ds gene provided in the present invention can be used to identify A. m. sinisxinyuan from other subspecies; can also be used for studying the genetic diversity of species resources of bees; and can further be used for studying stress resistance genes. This will fill the gap in the research field of A. m. sinisxinyuan by Chinese researchers.

A die cut tray insert is provided including a first face and a second face, opposite and parallel to the first face; first and second assemblies secured at one end to one of the first and second faces and configured to swing between an open position and a closed position such that a pot associated with a live sample is secured on two sides; and a third face and a fourth face, perpendicular to the first and second faces and opposite and parallel to one another, the third face configured to cover a top portion of the pot associated with the live sample and the fourth face configured to contain the pot within the first, second and third faces. The third face covering the top portion of the pot is configured to reduce spillage from the pot when the first and second assemblies are in a closed position.

The present invention relates to methods of facilitating the expression of recombinant polypeptides from cells, extracellular fluids, extracellular fibers, or any combination thereof, obtained from transgenic insect cells and larvae comprising a bacterial GlcNAc-6-P 2′-epimerase (GNPE), which is capable of converting N-acetyl-D-glucosamine-6-phosphate (GlcNAc-6-P) to N-acetyl-D-mannosamine-6-phosphate (ManNAc-6-P). The invention relates to methods to promote efficient glycoconjugate sialylation, by providing simpler ways to produce large intracellular pools of sialic acid precursors. The invention is also directed to nucleic acids, vectors, and cells comprising nucleic acids encoding polypeptides involved in the synthesis of sialic acid precursors, and cells in combination with nucleic acids encoding glycosyltransferases, including sialyltransferases, to facilitate the production of humanized recombinant glycoproteins. The engineered cells can be used to produce glycosylated proteins lepidopteran insects and cultured cell lines derived from Spodoptera frugiperda, Trichoplusia ni, and silkworms, such as Bombyx mori, particularly those that can be infected by baculovirus expression vectors.

A diffuser for distributing insects such as mosquitoes from an aircraft, comprises a distribution tube connected to an insect source at a first end and open to the outside at a second end in a direction away from that of travel of the aircraft, for distribution of the insects; and a profile surrounding the second end, the profile being shaped to define a steadily changing airspeed. The pipe may be aligned with airflow at the point of exit of the pipe and protection may be provided around or just upstream of the exit to provide a point of minimal air velocity at the exit, the exit being the point of release of the insects.