Materials and methods for use of constrained cohydration agents in the purification of biological materials such as antibodies, viruses, cells, and cellular organelles in connection with convective chromatography, fluidized bed or co-precipitation applications.

The invention is directed to utilization of a series of cross-linked 1,4-benzenediamine-co-alkyldiamine polymers and the use of the polymers to remove mercury from a hydrocarbon in fluid form.

A system is provided for removing arsenic from water to safe levels at or below the EPA standards. The system is a hybrid spouted vessel/fixed bed filter system that significantly enhances/improves arsenic removal for drinking water using zero-valent iron (ZVI) particles. Movement of the circulating, iron-containing particles in a dense moving bed that forms on the spouted vessel bottom creates an abrasive “self-polishing” action among them that continuously generates colloidal iron corrosion products. This material then circulates with the water in the vessel and is removed and concentrated in a fixed bed filter. The colloidal material captured and immobilized in the filter has been shown to remove arsenic from contaminated water at very rapid rates.

The present invention relates to a method of extracting a pigment from microalgae.

This invention relates generally to a process for selective adsorption and recovery of lithium from natural and synthetic brines, and more particular to a process for recovering lithium from a natural or synthetic brine solution by passing the brine solution through a lithium selective adsorbent in a continuous countercurrent adsorption and desorption circuit.

A spiral tube countercurrent chromatography rotor for separating virus in a two part aqueous solvent is described.

The present disclosure provides a method for extracting and separating flavonoids from Lindera aggregata leaves. The method includes: mixing Lindera aggregata leaves with an adsorbent, conducting elution with a matrix solid-phase dispersion (MSPD) extraction method, followed by concentration to obtain a Lindera aggregata leaf extract; conducting primary separation and secondary separation on the Lindera aggregata leaf extract by high-speed counter-current liquid chromatography (HSCCC), to separate quercetin-3-O-β-D-arabinofuranoside, a mixture of quercetin-3-O-β-D-glucoside and quercetin-5-O-β-D-glucoside, quercetin-3-O-rhamnopyranoside, and kaempferol-7-O-α-L-rhamnopyranoside; where a second solvent system used in the secondary separation includes ethyl acetate, n-butanol, an addictive and water, and the addictive includes cyclodextrin. The method has a short separation period, high separation efficiency, and less impurities during purification and separation of the flavonoids from the Lindera aggregata leaves.

A countercurrent chromatography method for separating cannabinoids using, for example, a solvent of hexane, methanol, ethyl acetate and water for separating THC and/or CBD, is provided.

Chromatography apparatus and methods are described, especially for expanded bed adsorption. A column tube has a process fluid input device at the bottom and a movable piston in the top. The piston is enclosed in the column by a cover plate. The piston body has an inflatable seal, and is connected by a frame to a contact ring which carries another inflatable member to contact the tube wall. Process fluid leaves the operating volume through an opening of the piston and flexible hose, through the enclosed space and out through the cover plate. The space above the piston can be pressurised to control piston movement. The contact ring maintains piston alignment. The inflatable seals are used to fix the piston in position, allow it to slide or allow washing. The piston outlet may include a vortex-inhibitor. Bed and piston levels may be monitored by ultrasound sensors.

The invention relates to a method for the chromatographic purification of at least one cannabinoid compound, wherein the method comprises a main purification stage comprising the steps of: injecting an initial mixture comprising the at least one cannabinoid compound and one or more additional compounds onto a main stationary phase comprising silica particles, the silica particles comprising amino and/or diol groups; performing an elution with an elution solution, and collecting one or more elution fractions; and optionally, washing the main stationary phase with a washing solution and collecting one or more washing fractions; at least one of the elution fractions or washing fractions containing the at least one cannabinoid compound purified from the one or more additional compounds.