A process is presented for the purification of an olefins feed stream to a benzene alkylation unit. The process removes heavy aromatics in a two adsorbent unit system. The unit passes the olefins feed stream to a first adsorbent unit, while the second adsorbent unit is either in regeneration mode, or standby mode. The process switches the feed stream to the second adsorbent unit and displaces the fluid in the second adsorbent unit, while maintaining the flow of the purified feed stream to the benzene alkylation unit.

One exemplary embodiment can be a process for treating a hydrocracking fraction. The process can include obtaining a bottom stream from a fractionation column, stripping HPNAs from the bottoms stream and adsorbing HPNAs from the stripped stream to provide an adsorbed stream that can meet a desired HPNA concentration specification. The adsorption step can be adjusted to achieve an adjusted HPNA concentration.

The invention provides a high-performance liquid chromatography system, said system is controlled in temperature by running a fluid in sleeves that surround the different parts of the system. All parts in contact with the fluid are made in fluoropolymer, carbon-filled fluoropolymer, or carbon-fiber fluoropolymer. The system comprises at least one reagent reservoir; at least one mixing chamber, wherein the contents of the at least one reagent reservoir are combined; at least one pump that transfers the contents of the at least one reservoir to the mixing chamber; and at least one modular elution column, wherein the at least one modular elution column contains a temperature control means; a sample injection system connected to an injection loop or 3-way valve to inject the sample solutions in the modular elution columns, at least one manifold or X-Y moving stage to distribute the eluted volumes in different receptacles; at least one return line to automatically reinject selected elution fractions at the top of the column; wherein all moving components of the said system are fluid actuated.

The present invention comprises a novel multimodal chromatography sequence of short length alternating adsorption and size exclusion media operating with gradient elution. The novel multimodal chromatography in an oscillating series utilizes the alternating solvent exchange media to reposition the active region of separation back in phase with the target solutes.

Each solvent exchange column bed length in the sequence is designed to achieve a subtle decrease or increase in the solvent gradient (or salt gradient) concentration associated with the two solutes of interest which results in an extension of the active separation or increasing differences in solute velocity for two solutes of interest.

The novel oscillatory chronographic system demonstrates much improved separation capability as shown by a one dimensional model.

The disclosure features methods that include obtaining a vibrational spectrum of a solution in a biological manufacturing system, analyzing the vibrational spectrum using a first chemometrics model to determine a value of a first quality attribute associated with the solution, analyzing the vibrational spectrum using a second chemometrics model to determine a value of a second quality attribute associated with the solution, and adjusting at least one parameter of a purification unit of the biological manufacturing system based on at least one of the values of the first and second quality attributes.

A method for separating a lead radioisotope from a mixture comprising the lead radioisotope and a radioisotope of radium or thorium is provided, along with a system comprising a plurality of chromatographic columns. The system can include a first cartridge having a lead-complexing media that preferentially binds the lead radioisotope over radioisotopes of radium or thorium, and a second cartridge having a weak cationic exchange media, where a pH of a loading solution used to load the second cartridge is pH.sup.2L, and a pH of an eluent used to elute the lead radioisotope from the second cartridge is pH.sup.2E, and pH.sup.2L is greater than pH.sup.2E. The system can also comprise further third and fourth cartridges with chromatographic media to extract and purify the lead radioisotope, to provide a purified solution of lead radioisotope that can be used for medical and other purposes, such as in the labeling of radiopharmaceutical compounds.

A cyclic chromatographic purification method for the isolation of a product from a feed mixture comprising the product and at least two further components representing weakly adsorbing impurities and strongly adsorbing impurities, the method using only two chromatographic adsorber sections as chromatographic stationary phase. The method comprises at least one base sequence having at least one Recycling Phase followed by only one Purification Phase, wherein preferably base sequences repeat in a cyclic manner.

A method for separating a lead radioisotope from a mixture comprising the lead radioisotope and a radioisotope of radium or thorium is provided, along with a system comprising a plurality of chromatographic columns. The system can include a first cartridge having a lead-complexing media that preferentially binds the lead radioisotope over radioisotopes of radium or thorium, and a second cartridge having a weak cationic exchange media, where a pH of a loading solution used to load the second cartridge is pH.sup.2L, and a pH of an eluent used to elute the lead radioisotope from the second cartridge is pH.sup.2E, and pH.sup.2L is greater than pH.sup.2E. The system can also comprise further third and fourth cartridges with chromatographic media to extract and purify the lead radioisotope, to provide a purified solution of lead radioisotope that can be used for medical and other purposes, such as in the labeling of radiopharmaceutical compounds.

The disclosure features methods that include obtaining a vibrational spectrum of a solution in a biological manufacturing system, analyzing the vibrational spectrum using a first chemometrics model to determine a value of a first quality attribute associated with the solution, analyzing the vibrational spectrum using a second chemometrics model to determine a value of a second quality attribute associated with the solution, and adjusting at least one parameter of a purification unit of the biological manufacturing system based on at least one of the values of the first and second quality attributes.

A dynamic mixing disk assembly for a centrifugal platform has an inner disk and an outer disk. The inner disk comprises a first ring part and a second ring part configured to an outer periphery circumference of the first ring part. The first ring part comprises multiple or at least two pawls. The outer disk is rotatably disposed on the second ring part of the inner disk. The outer disk comprises multiple tapered recesses or at least two tapered recesses. When the pawls are engaged with the tapered recesses of the outer disk, the inner disk and the outer disk are able to rotate synchronously with the centrifugal platform. When the inner disk is stopped by the centrifugal platform, the outer disk will keep rotating, and the pawls disengage from the tapered recesses and move to the next tapered recesses.