A composite sorbent composition comprising a polymeric adsorbent; and an extractant having the formula (I), or hydrate in thereof, wherein X is O or S, A1 and A2 are each independently —C(O)— or —C(R′)(R″)— wherein R′, and R″ are each independently hydrogen, halogen, hydroxyl, cyano, nitro, amino, —CHO, —COOH, C1-12 alkyl, C1-4 alkoxy, C1-4 alkylamino, C1-2 haloalkyl, C1-2 haloalkoxy, C1-12 cycloalkyl, C6-12 aryl, C7-13 arylalkyl, C3-12 heteroaryl, C1-12 heteroalkyl, or C4-12 heteroarylalkyl, Z is a covalent bond, —S—, —O—, —SO2—, —SO—, —P(R)(═O)—, —NR—, -C(O)-, -C(O)NH-, —C(═N—R)—, or —C(R′)(R″)— wherein R, R′, and R″ are each independently hydrogen, halogen, hydroxyl, cyano, nitro, amino, —CHO, —COOH, —C(O)NH2, C1-12 alkyl, C1-12 alkoxy, C1-12 alkylamino, C1-4 haloalkyl, C1-4 haloalkoxy, C4-12 cycloalkyl, C6-12 aryl, C7-13 arylalkyl, C3-12 heterocycloalkyl, C3-12 heteroaryl, C1-12 heteroalkyl, or C4-12 heteroarylalkyl, and R1 and R2 are each independently hydrogen, halogen, hydroxyl, cyano, nitro, amino, or a substituted or unsubstituted monovalent C1-40 hydrocarbon.

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The present disclosure provides processes and systems for ethanol production. In one embodiment, a first beer column receives a first portion of a feed mixture including ethanol and water to form a first beer column bottom stream and a first beer column vaporous overhead stream. A beer column receives a second portion of the feed mixture. A first portion of the first beer column bottom stream is forwarded to a first beer column reboiler. A second portion of the first beer column bottom stream is forwarded to a plurality of evaporators. A condensed portion of the first beer column vaporous overhead stream is forwarded to a stripper column. The stripper column forms a feed stream, which is contacted with a separation system, thereby forming a permeate and a retentate. The permeate is forwarded directly to at least one selected from the first beer column and the stripper column.

The present disclosure provides processes and systems for dehydrating a byproduct stream in ethanol production. In one embodiment, a feed mixture is distilled with one or more distillation units to remove at least a portion of the water, and form a first byproduct stream. The first byproduct stream is contacted with a molecular sieve unit, thereby forming a product stream. The molecular sieve unit is cyclically contacted with at least a portion of the product stream to regenerate the molecular sieve unit and form one or more regenerate streams. A second byproduct stream including at least one of (1) the regenerate streams and (2) at least a portion of the fusel oil stream is contacted with a separation system, thereby forming a permeate and a retentate. At least a portion of the retentate is forwarded into the product stream.

Disclosed herein are embodiments of an extractant that can be used for chromatographic isolation of radioisotopes and/or metal species. The extractant can be combined with a support medium to provide an extractant composition that selectively and efficiently binds particular radioisotopes and/or metal species. Also disclosed herein are embodiments of a method for using the disclosed extractant embodiments, as well as embodiments of a method for making the extractant and extractant composition.

The invention relates to the field of protein purification processes involving several chromatography steps. The invention pertains to a method for purifying a protein, preferably an antibody or fragment thereof or a protein containing said fragment, from a complex solution, wherein said method comprises at least two chromatography steps which are performed using buffers comprising or consisting of the same chemical compounds. The invention is particularly useful for large scale production and purification of recombinant proteins.

The present invention provides methods for cleaning or regenerating a chromatography materiel for reuse. The methods of the invention can be used for cleaning or regenerating chromatography columns for reuse in the large-scale manufacture of multiple polypeptide products.

The invention provides columns (including pipette tip columns) and automated methods for the purification of nucleic acids including plasmids. Nucleic acids can be purified from unclarified, clarified or partially-clarified cell lysates that contain cell debris. The columns typically include a bed of medium positioned above a bottom frit and with an optional top frit. Plasmid preparation scales include miniprep, midiprep, maxiprep, megaprep and gigaprep.

Systems and methods for selectively recovering a target metal from an ion exchange resin are generally described. In certain embodiments, such methods and systems can be employed for metal purification and enrichment of target metal species from mixtures containing contaminating or non-target metals. In some embodiments, ion exchange is accomplished in the presence of one or more species that facilitate the recovery of a metal from a composition further comprising one or more other metals. The recovered metal-containing composition may contain the recovered metal at relatively high purity and/or in relatively large amounts. In some embodiments, the conditions present during ion exchange are varied in a manner that facilitates the enrichment of a metal from an initial (e.g. sample) composition further comprising one or more other metals to result in a product that contains the enriched metal at relatively high purity and/or in relatively large amounts

This invention relates to a method of removing a chemical entity from a liquid using affinity chromatography. The method involves passing an elongate solid phase through a conduit through which the liquid also flows.

An aqueous modified acid composition for industrial activities, said composition comprising: an alkanolamine and strong acid in a molar ratio of not less than 1:15, preferably not less than 1:10; it can also further comprise a metal iodide or iodate. Said composition demonstrates advantages over known conventional acids and modified acids.