A preparative closed cycle supercritical fluid column chromatography system, device, and method of isolating high volumes of pure components from mixtures using a supercritical solvent. Bulk fractions of desirable material from plants can be obtained using supercritical fluid column chromatography with a chromatography column. A chemical sensor downstream the chromatography column detects chemical species eluted from the column and a plurality of collection columns collects the bulk fractions of product with a control system controlling the collection valves based on detection of chemical species at the chemical sensor.

A method for fraction collection and a fraction collection system (100) comprising: —a dispensing device (23) including an outlet (24) configured for dispensing fractions of a liquid received from a connected system; —plural receptacles (25) configured for receiving one or more of said fractions, wherein the receptacles (25) and the dispensing device (23) are movable in relation to each other into a number of different positions such that the dispensing device (23) is capable of dispensing a respective fraction of said liquid into one or more of the receptacles (25); —a waste collection device (27), which is arranged in the fraction collection system (100) such that it is movable into at least a first and a second position wherein, in said first position, an inlet (29) of the waste collection device (27) is positioned below the outlet (24) of the dispensing device (23) such that liquid being dispensed from the outlet (24) of the dispensing device (23) is received in the waste collection device (27) and wherein, in the second position, the inlet (29) of the waste collection device (27) is not positioned below the outlet (24) of the dispensing device (23) such that fractions being dispensed from the dispensing device (23) can be received in one or more of the receptacles (25).

Described are a fraction collector and a method of fraction collection for a liquid chromatography system. The method includes diverting a liquid chromatography system flow from a waste channel to a collection tube at a start of a fraction collection window and collecting the system flow dispensed from the collection tube during the fraction collection window. At the end of the window, the system flow is diverted to the waste channel and a flow of a wash solvent is provided to the collection tube to dispense liquid remaining in the collection tube at the end of the window from the collection tube. The method can increase the amount of the collected fraction and can reduce or eliminate cross-contamination of a subsequently collected fraction. The method is useful for analytical scale applications where the collected fractions may have volumes defined by a limited number of drops dispensed from the collection tube.

The invention relates to a fraction collector apparatus (100) comprising: a support system (1, 2), a carrier (42) moveably supported by the support system (1, 2), an extension arm (3) connected to the carrier (42), at least one dispensing head (31) for dispensing droplets and moveably connected to the extension arm (3), wherein the dispensing head (31) and (carrier 42) are configured to move relative to the support system in a first plane, a tray area (11) designed to support at least one rack (6, 7), wherein the at least one rack (6, 7) is designed to hold at least one collection vessel (12, 14), wherein motion of said carrier (42) is achieved by a linear bearing Y-slide unit (41) wherein the linear bearing Y-slide unit (41) is arranged underneath a linear bearing Y-rail (40) and wherein the carrier (42) is attached to the linear bearing Y-slide unit (41).

The invention is a method for liquid, gaseous or supercritical phase chromatography which involves circulating, on a chromatograph (6) containing a stationary phase, a load (1) comprising components to be separated entrained by a carrier fluid (2), said method being characterized in that it involves: (a) obtaining, at the outlet of the chromatograph, a plurality of chromatographic fractions (3, 4) comprising at least one component of the load and the carrier fluid in a first fluid phase, (b) imposing a change of state on at least one of said chromatographic fractions (3, 4) so as to obtain at least one fraction of purified carrier fluid in a second fluid phase different from the first fluid phase by separating said carrier fluid from the component of the load, (c) imposing a change of state in a reverse direction to that of step (b) on at least one fraction of purified carrier fluid obtained in step (b) so as to obtain at least one fraction of purified carrier fluid in a third fluid phase different to the second fluid phase, and in that it involves coupling the change-of-state energies from the first fluid phase to the second fluid phase and from the second fluid phase to the third fluid phase of the same or of another fraction of purified carrier fluid, said coupling comprising a transfer of heat using a heat pump.

A preparative liquid chromatograph including an analysis channel (2), an injector (6), a separation column (8), a detector (10), a plurality of fraction collectors (14-1 to 14-n), a switching valve (12) that switches fraction collectors (14-1 to 14-n) to be connected to the outlet of the detector (10) among the plurality of fraction collectors (14-1 to 14-n), and a controller (16) for controlling operations of the plurality of fraction collectors (14-1 to 14-n) and the switching valve (12). In a case where the number of remaining unused collection containers of the fraction collector in use, which is connected to the outlet of the detector, falls below the number of buffers which is an integer of two or more, the controller (16) is configured to switch the switching valve (12) while the fraction collector in use is not performing a peak collection operation.

Presteralized manifolds are provided which are designed for sterile packaging and single-use approaches. Disposable tubing and flexible-wall containers are assembled via aseptic connectors. These manifolds are adapted to interact with other equipment which can be operated by a controller which provides automated and accurate delivery of biotechnology fluid. The manifold also can be used in conjunction with one or more sensors such as pressure and conductivity sensors that interact with the controller or are connected to a separate user interface. An aseptic environment obtains avoiding or reducing cleaning and quality assurance procedures.

A preparative liquid chromatograph for separating and extracting components in a sample, the preparative liquid chromatograph including a separation channel (2) through which a mobile phase flows, an injector (6) that injects the sample into the mobile phase, a separation column (8) for separating components in the sample injected into the mobile phase, the separation column (8) being provided downstream of the injector (6) on the separation channel (2), a detector (10) for detecting peaks of the components separated by the separation column (8), a fraction collector (12) configured to collect an eluate from the separation column (8) into a plurality of collection containers (20:22) at a downstream of the detector (10) while fractionating the eluate, and a controller (14) for controlling operation of the fraction collector (12), wherein the fraction collector (12) is provided with peak collection containers (20) and waste liquid collection containers (22), each peak collection container (20) is used for collecting a peak portion including a collection target peak detected by the detector (10) in the eluate, and each waste liquid collection container (22) is used for collecting a waste liquid portion not including the collection target peak in the eluate, and the controller (14) is configured to collect the peak portion including the collection target peak detected by the detector (10) in the eluate in the peak collection container (20), and to collect at least a part of the waste liquid portion in the waste liquid collection container (22).

A method for preparing a Luo Han Guo sweetening composition from Siraitia grosvenorii and a use thereof. The method for extracting the sweetening composition from Siraitia grosvenorii preferably includes the followings: accelerating ripening of immature Siraitia grosvenorii, and performing juicing, extraction with pure water, removal of impurities, concentration and purification to obtain the sweetening composition. Further, the present application relates to a compound sweetener containing the sweetening composition, which can be widely used in foodstuffs, beverages, healthcare products, and daily chemicals. The contents of mogroside III, mogroside IIe, and the like in the Luo Han Guo sweetening composition are controlled so as to improve the flavor thereof, and a production process for the sweetening composition uses only pure water, without use of organic solvents such as ethanol, to ensure a greener and healthier production process.

A method of recovering paraxylene in a pressure swing adsorption unit with varying hydrogen purge pressures. The pressure swing adsorption zone is adapted to adsorb and desorb paraxylene based on the cycling of partial pressure in the zone. A first hydrogen purge is fed concurrent to the feed. A second hydrogen purge is countercurrent to the feed.