Problems to be Solved

The present invention provides an affinity support capable of trapping a substance by cooperative binding that is less likely to cause dissociation even when the substance is a molecule other than an antibody, and a trapping method using the same.

Means to Solve the Problems

A method of trapping a substance comprising the step of contacting an objective to be trapped with an affinity support comprising a support, a spacer bound to the support and an affinity substance bound to the spacer, so as to bind the objective to be trapped to the affinity substance, wherein each one of the objective to be trapped has a plural of affinity sites and the affinity substance binds to at least two of the affinity sites simultaneously.

A microfluidic chip for on-chip detection of the presence or absence of a target nucleic acid region in an isolated nucleic acid sample is disclosed. The microfluidic chip includes a nucleic acid entanglement array, an isolated nucleic acid sample immobilized in the nucleic acid entanglement array, and at least one probe specific to a target nucleic acid region. Systems and methods of using the microfluidic chip are disclosed. An integrated microfluidic cell processing system is disclosed, which includes: a multiplexed microfluidic flow directing system having a plurality of reconfigurable microfluidic layers that form a plurality of reconfigurable microfluidic channels, where the multiplexed microfluidic flow directing system function to assist in directing flow of materials into, through, and out of the integrated cell processing system; and at least one microfluidic chip functionally integrated into at least one layer of the multiplexed microfluidic flow directing system, and operates under continuous flow conditions.

The present invention relates to a polymer including at least one structure selected from the group consisting of a structure represented by General Formula (3) described below and a structure represented by General Formula (4) described below:

##STR00001## in General Formula (3) and General Formula (4) described above, X.sub.31 and X.sub.41 represent a hydrophilic group-containing structure, n represents an integer of 0 to 2, R represents a hydrogen atom or an alkyl group, Y.sub.31 to Y.sub.32 and Y.sub.41 to Y.sub.43 each independently represent a hydrophilic group-containing structure, a hydrogen atom, or an alkyl group.

The present disclosure relates to separation and purification methods for a vaccine virus using affinity chromatography, and more particularly, to a purification method for a virus capable of obtaining a vaccine virus with a high purity and a high yield using affinity chromatography containing a vaccine virus-affinity resin.

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

A method for recovering a parent radionuclide from a radionuclide generator is disclosed where the parent radionuclide is adsorbed to a stationary phase. The method contains a series of elutions. At least one elution is with an alcohol. At least one elution with water. At least one elution is with a mineral acid other than hydrochloric acid that is paused to soak the stationary phase with the mineral acid.

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.

A two-step chromatography purification scheme is described which selectively captures and isolates the genome-containing rAAV vector particles from the clarified, concentrated supernatant of a rAAV production cell culture. The process utilizes an affinity capture method performed at a high salt concentration followed by an anion exchange resin method performed at high pH to provide rAAV vector particles which are substantially free of rAAV intermediates.