The present invention relates to a method for preparing cannabidiol by separation and purification using high-speed countercurrent chromatography, comprising: alcohol extraction and water precipitation, adsorption with a macroporous resin, and high-speed countercurrent chromatography separation. The present invention separates and obtains high-purity cannabidiol from industrial hemp flowers or leaves, while at the same time removing the psychotoxic component tetrahydrocannabinol by combining a macroporous resin chromatographic column with a high-speed countercurrent chromatograph, and optimizing process parameters, and the solvent used therein being environmentally friendly, leaving no residues, having low cost and being recyclable. Therefore, the method is suitable for industrial production.

The patent discloses a method for efficiently collecting and purifying Orobanche cumana (O. cumana) germination stimulants using aeroponic system and solid-phase extraction (SPE), including the following steps: (1) sunflower seeds germination, then planting sunflower seedlings in aeroponic device, and cultivating the sunflower seedlings in the aeroponic system; at the aeroponic stage, phosphorus-containing aeroponic nutrient solution is first used to cultivate the sunflower seedlings for 20 to 25 days, and phosphorus-free aeroponic nutrient solution is then used instead to subject the sunflower seedlings to starvation cultivation for 5 to 7 days; and (2) passing all nutrient solutions in the aeroponic device through an SPE cartridge for SPE to extract O. cumana germination stimulants. The obtained O. cumana germination stimulants are diversified, and have high concentration and purity.

Methods of preparing steviol glycosides, including Rebaudioside D, Rebaudioside E, Rebaudioside M, Rebaudioside N and Rebaudioside O are provided herein. Sweetener and sweetened consumables containing Rebaudioside D, Rebaudioside E, Rebaudioside M, Rebaudioside N and Rebaudioside O are also provided herein.

The present invention relates to the field of laboratory-scale sample preparation, which describes a methodology for the specific isolation of tetraprotic naphthenic acids, called ARN acids, from residual naphthenate deposits from petroleum production. The method consists of cleaning the naphthenate deposit, converting the naphthenate salts to naphthenic acids and isolating the ARN acids from the other organic acids, using a silica-based sorbent material with aminopropyl functional groups, previously selected for an efficient elution of different functional groups and polarities.

[Problems] To provide a method for treating hydroxyapatite filler so that it can be used multiple times in the separation of a charged material included in a sample liquid using adsorbent composed of the hydroxyapatite filler, a production method including the treatment method, and hydroxyapatite filler.

[Means to solve problems] The treatment method of the present invention comprises a first step of bringing a first liquid containing a predetermined material into contact with hydroxyapatite filler, and a second step of bringing a second liquid containing an alcohol into contact with the hydroxyapatite filler.

The present disclosure discloses a 1-carboxy-2-hydroxy-3-iminopropane and an extraction method thereof, and belongs to the technical fields of food, health food and medicine. A method for extracting the 1-carboxy-2-hydroxy-3-iminopropane of the present disclosure includes the following steps: (1) adding a diaphragma juglandis fructus powder into an ethanol solution for extraction and filtration to obtain a supernatant, and conducting concentration on the supernatant under reduced pressure to obtain a walnut alcohol extract; (2) adding a filter residue of step (1) into water for extraction and filtration to obtain a supernatant, and conducting concentration on the supernatant under reduced pressure to obtain a walnut water extract; and (3) mixing the walnut alcohol extract and the water extract to obtain a diaphragma juglandis fructus mixed extract, sequentially conducting elution through an AB-8 type macroporous resin column, an MCI column and an ODS column, and conducting separation to obtain a precipitate, namely the 1-carboxy-2-hydroxy-3-iminopropane. In the present disclosure, the 1-carboxy-2-hydroxy-3-iminopropane in diaphragma juglandis fructus is found for the first time and has great sedation and hypnosis activity.

The invention relates to the field of separating rare earth elements by chromatography. The claimed method for separating lutetium and ytterbium from acidic solutions resulting from the recycling of irradiated ytterbium-176 targets is carried out using ion exchange chromatography. Ion sorption is performed on a sulphonic cation resin in copper or nickel form, and lutetium and ytterbium are eluted using a solution of a chelator at elevated temperature with the aid of a system consisting of at least two series connected columns of decreasing diameter, filled with a sulphonic cation resin. The separation of ytterbium and lutetium is carried out in the presence of a bivalent ion of a stable intercalator element selected from the group consisting of cobalt, lead or zinc, in an amount in mEq that is equal to not less than 80% of the total capacity of the last column in the direction of travel of the solution. A solution of ethylenediaminetetraacetic acid or nitrilotriacetic acid is used as the eluent. The technical result is that of increasing the degree of chromatographic separation of lutetium and ytterbium from acidic solutions resulting from, the recycling of ytterbium-176 targets, including targets having a mass greater than 10 g, as well as expanding the range of methods available for separating the aforesaid components.

A method of purification and/or separation of glycopeptides and quantitation of same. The method includes contacting a sample comprising glycopeptides to a hydrophilic enrichment substrate under conditions that permit the glycopeptides to bind to the hydrophilic enrichment substrate. The glycopeptides are eluted from the hydrophilic enrichment substrate with an ammonium formate and acetonitrile (ACN) in water solution to create an enriched glycopeptide sample, which may be subjected to analysis to identify specific glycopeptides.

A process for separating one or more one-ring cycloparaffins and one or more multi-ring cycloparaffins from a hydrocarbon mixture is disclosed. The process comprises the steps of providing the hydrocarbon mixture; and contacting the hydrocarbon mixture with an adsorbent material comprising a metal organic framework to separate the one or more one-ring cycloparaffins and the one or more multi-ring cycloparaffins from the hydrocarbon mixture. The process is conducted in a liquid phase.

The present disclosure relates to multistep chromatographic methods for preparing extracellular vesicles (EVs). The methods were demonstrated to be effective in preparing high quality EVs in a large scale. The methods enable preparation of EVs for therapeutic and diagnostic applications, and isolation and/or sub-fractionation of EVs with desired properties for specific use.