Provided herein are methods and formulations for stable and prolonged storage of infective juveniles of entomopathogenic nematodes.

Provided herein are methods and formulations for stable and prolonged storage of infective juveniles of entomopathogenic nematodes.

The present application is directed to a system and device for attraction of pests. This system comprises CO.sub.2-releasing microorganisms, and also nutrients specific to these microorganisms, and this system, or the device, comprises one or more biodegradable biopolymers and allows CO.sub.2, and optionally other attractants, to be released over a period of more than 20 days. Furthermore, the present application provides for the use of such a system or such a device for the attraction of pests, more particularly of maize or potato pests, such as larvae of the Western corn rootworm or wire worm. The present application is also directed, lastly, to methods for attracting pests, more particularly pests of maize or potatoes, such as the larvae of the Western corn rootworm, or for wire worms, with the systems or devices of the invention being positioned in the immediate vicinity of the plants, but preferably not directly on the plants.

The present application is directed to a system and device for attraction of pests. This system comprises CO.sub.2-releasing microorganisms, and also nutrients specific to these microorganisms, and this system, or the device, comprises one or more biodegradable biopolymers and allows CO.sub.2, and optionally other attractants, to be released over a period of more than 20 days. Furthermore, the present application provides for the use of such a system or such a device for the attraction of pests, more particularly of maize or potato pests, such as larvae of the Western corn rootworm or wire worm. The present application is also directed, lastly, to methods for attracting pests, more particularly pests of maize or potatoes, such as the larvae of the Western corn rootworm, or for wire worms, with the systems or devices of the invention being positioned in the immediate vicinity of the plants, but preferably not directly on the plants.

Entomopathogenic nematode Heterorhabditis bacteriophora having an enhanced longevity, comprising a first locus comprising a single nucleotide polymorphism at position 75 of the nucleotide sequence SC00004647 as depicted in SEQ ID NO: 5, in which C is substituted by T; and/or a second locus comprising a single nucleotide polymorphism at position 54 of the nucleotide sequence SC00006203 as depicted in SEQ ID NO: 7, in which C is substituted by T.

Entomopathogenic nematode Heterorhabditis bacteriophora having an enhanced longevity, comprising a first locus comprising a single nucleotide polymorphism at position 75 of the nucleotide sequence SC00004647 as depicted in SEQ ID NO: 5, in which C is substituted by T; and/or a second locus comprising a single nucleotide polymorphism at position 54 of the nucleotide sequence SC00006203 as depicted in SEQ ID NO: 7, in which C is substituted by T.

Disclosed are methods for increasing infectivity of entomopathogenic nematodes in any target area for the purposes of insect control, said method comprising: (a) mixing an aqueous nematode infectivity increasing composition with (i) entomopathogenic nematodes to activate said entomopathogenic nematodes for increased infectivity prior to application of said entomopathogenic nematodes to said target area, or (ii) seeds to produce coated seeds, and (b) applying said entomopathogenic nematodes to said target area or planting said coated seeds in said target area; wherein said aqueous nematode infectivity increasing composition is produced by methods disclosed herein.

Disclosed are methods for increasing infectivity of entomopathogenic nematodes in any target area for the purposes of insect control, said method comprising: (a) mixing an aqueous nematode infectivity increasing composition with (i) entomopathogenic nematodes to activate said entomopathogenic nematodes for increased infectivity prior to application of said entomopathogenic nematodes to said target area, or (ii) seeds to produce coated seeds, and (b) applying said entomopathogenic nematodes to said target area or planting said coated seeds in said target area; wherein said aqueous nematode infectivity increasing composition is produced by methods disclosed herein.

The invention relates to the use of Panagrolaimus sp. for feeding useful insects or in a method for controlling plant-damaging arthropods by applying a Panagrolaimus sp.-containing, useful insect- and mite-promoting feed stuff at the location of at least one plant species.

The invention relates to the use of Panagrolaimus sp. for feeding useful insects or in a method for controlling plant-damaging arthropods by applying a Panagrolaimus sp.-containing, useful insect- and mite-promoting feed stuff at the location of at least one plant species.