The invention relates to inorganic particles which are covalently bonded to first polymer chains made up of at least one polymer carrying proton exchange groups, optionally in the form of salts, and bonded to second polymer chains made up of at least one fluorinated polymer that does not carry any proton exchange groups, the second chains being bonded to the particles via organic spacer groups, or the second chains being bonded to first chains via organic spacer groups, or some of the second chains being bonded to the particles via organic spacer groups while the remaining second chains are bonded to first chains via organic spacer groups.

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.

An article that can be used for biomaterial capture comprises (a) a porous substrate; and (b) borne on the porous substrate, a polymer comprising interpolymerized units of at least one monomer consisting of (1) at least one monovalent ethylenically unsaturated group, (2) at least one monovalent ligand functional group selected from acidic groups, basic groups other than guanidino, and salts thereof, and (3) a multivalent spacer group that is directly bonded to the monovalent groups so as to link at least one ethylenically unsaturated group and at least one ligand functional group by a chain of at least six catenated atoms.

An article that can be used for biomaterial capture comprises (a) a porous substrate; and (b) borne on the porous substrate, a polymer comprising interpolymerized units of at least one monomer consisting of (1) at least one monovalent ethylenically unsaturated group, (2) at least one monovalent ligand functional group selected from acidic groups, basic groups other than guanidino, and salts thereof, and (3) a multivalent spacer group that is directly bonded to the monovalent groups so as to link at least one ethylenically unsaturated group and at least one ligand functional group by a chain of at least six catenated atoms.

Provided is a manufacturing method for monolithic structure that is a porous body formed of polysaccharide being a naturally-occurring polymer, has continuous pores with an average pore diameter suitable for biomolecule separation, and allows formation into arbitrary shape. The polysaccharide monolithic structure is manufactured by a method including a first step of obtaining a polysaccharide solution by dissolving polysaccharide into a mixed solvent of a first component and a second component at temperature lower than a boiling point of the mixed solvent, and a second step of obtaining polysaccharide monolithic structure by cooling the polysaccharide solution, wherein the first component is a solvent selected from lactate, and the second component is a solvent selected from water, lower alcohol, and a combination thereof. The monolithic structure obtained is a porous body having continuous pores with an average pore diameter of 0.01 to 20.0 micrometers, and a thickness of 100 micrometers or more.

In one aspect, a functionalized porous material includes a synthetic porous material, the synthetic porous material comprising a surface, wherein at least a portion of the synthetic porous material surface is a substrate; and a mixed-metal hydroxide, wherein the mixed-metal hydroxide is affixed to the substrate.

Methods of using a separation media to isolate a target molecule that includes a carbohydrate are disclosed. The separation media includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity capable of recognizing and binding to a carbohydrate.

The present invention provides novel chromatographic materials. e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The chromatographic materials of the invention have controlled porosity and comprise a chromatographic core material and one or more layers of chromatographic surface material which each independently provide an average pore diameter, an average pore volume, or a specific surface area such that the combined layers form a chromatographic material having a predetermined or desired pattern of porosity from the core material to the outermost surface. The materials are useful for HPLC separations, normal-phase selarations, reversed-phase separations, chiral separations, HILIC separations, SFC separations, affinity separations, perfusive separations, partially perfusive separations, and SEC separations.

The invention relates to chromatographic separating materials having improved binding capacity for biological constituents in cell culture supernatants, or animal or human body fluids, in particular for monoclonal antibodies. The present invention likewise relates to the preparation of separating materials of this type, and to the use thereof, in particular for the removal of charged biopolymers from corresponding liquids.

A three-component composition for use in the treatment of preeclampsia and related disorders wherein a first component comprises a bimodal synthetic carbon particle mixture; a second component comprises a resin bead with at least one affinity ligand directed toward syncytiotrophoblast-derived factor sEndoglin and a third component comprises a resin bead with at least one affinity ligand directed toward syncytiotrophoblast-derived factor soluble Fms-like tyrosine kinase-1.