Aspects of the present invention provide a modular reactor device having an outer housing, and a plurality of components contained within the outer housing, the components including: a reaction chamber; a fluid pathway connected to the reaction chamber; and a valve arranged to control flow of fluid within the device, wherein the outer housing has a plurality of connection ports providing connections from the exterior of the device to the interior, the connection ports including: a fluid input and a fluid output; an electrical input; and a pneumatic input; wherein either the electrical input or the pneumatic input is connected to the valve to provide for control of the valve, and either the fluid input or the fluid output is connected to the reaction chamber or the fluid pathway. Other aspects provide a base station for receiving and controlling a modular reactor device and methods for manufacturing the modular reactor device and for performing reactions using a modular reactor device.

The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. opened or closed), differential fluid pressures at circuit inlets or upstream reservoirs, flow path resistances, and the like. Free diffusion or leakage of fluids from unselected inlets into the common outlet or other inlets at junctions or nodes is prevented by the flow of the selected inlet fluid, a portion of which sweeps by the inlets of unselected fluids and exits the fluidics circuit by waste ports, thereby creating a barrier against undesired intermixing with the outlet flow through leakage or diffusion.

Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.

The invention relates to a container holder 10, comprising a main body 12 which in turn comprises a gas inlet 16; a solution liquid outlet 18; a gas control valve 20 through which a gas enters the container 100 from the gas inlet; and a sealing means 22 for the container, which sealing means includes a passageway 24 for the input of gas and output of a solution in the container via an egress tube 19; wherein when the container is connected to the container holder through the sealing means, the gas control valve opens automatically, and when the container is disconnected the gas control valve is closed automatically. The invention further relates to a container panel 50 which includes two or more container holders. Also disclosed are methods of using these containers and container panels for synthesizing a polypeptide.

There is provided a sample ejection system for ejecting a sample from a flow of fluid, the system comprises: a fluid flow channel for receiving, in use, the flow of fluid; a fluid inlet channel connected to the flow channel and arranged to receive, in use, pressurised fluid; a fluid outlet channel connected to the flow channel at a location downstream in the flow direction of the flow of fluid and comprises an outlet. The system is arranged such that, in use, pressurised fluid is applied to the fluid flow channel via the fluid inlet channel to drive fluid from the region of the fluid flow channel between the fluid inlet and outlet channels through the fluid outlet channel and out of the outlet.

An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites.

The present invention relates to a method of catalytic process characterization which comprises a reaction system having two or more reaction strands in a parallel arrangement, wherein an individual reaction strand comprises multiple series-connected reaction chambers or a single reaction chamber. In the method, which is also referred to as CPC method, each reaction strand is supplied with a reactant stream. The reactant streams supplied to the reaction strands are subjected to different numbers of process stages in the different reaction strands. The product streams discharged from the reaction strands are subjected to an analytical characterization, wherein the data achieved in the characterization are expressed in relative terms, here preferably including the forming of a difference. The CPC method can be used in a very versatile manner and is characterized by very high accuracy. The mass balance achieves a standard deviation of +/10% by weight or lower. Furthermore, the invention relates to an apparatus for performing the CPC method or else to an apparatus for simultaneously performing a multitude of CPC methods. The invention thus also relates to the field of high-throughput research.

An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites.

An apparatus and a method are used for investigating the naphtha reforming process in catalyst test devices with reactors arranged in parallel. The apparatus has a plurality of reactors arranged in parallel with reaction chambers (R1, R2, . . . ), a product fluid supply, a process control, and at least one analysis unit. Each individual reactor has an outlet line for the product fluid stream, wherein the analysis unit is operatively connected to each outlet line for the product fluid stream and the apparatus is functionally connected to the control of the apparatus. In carrying out the method, naphtha-containing reactant fluid streams are brought into contact with catalysts in the individual reactors and the product fluid streams are subsequently supplied to the online analysis unit from the respective outlet lines of the individual reactors and analyzed. Using the evaluation of the online analytical characterization data, the process parameters of the respective reactor unit are adapted. The process steps of analytical characterization, evaluation, and adaptation of process parameters are repeated for the duration of the investigation.

A synthesis device comprises a plurality of pipes, a feeding unit, a reaction vessel, and a measurement mechanism. The pipes extend from a plurality of storage containers, respectively, in which a plurality of types of solutions are stored. The feeding unit is configured to feed the solutions in the storage containers through the pipes. The solutions selectively fed from the storage containers are put in the reaction vessel to generate a synthesized product by chemical synthesis. The measuring mechanism is provided between the storage containers and the reaction vessel in a middle of an overall flow path including the pipes, the measuring mechanism being configured to measure the solutions fed to the reaction vessel.