A multi-channel direct-deposit assembly method is disclosed to high-throughput synthesize three-dimensional macroporous/mesoporous (3DMM) material array with precisely controlled composition, pore size, and pore structure. The macropore size of the synthesized 3DMM material is in the range of 50-1000 nm; the mesopore size of the synthesized 3DMM material is in the range of 1-50 nm. The surface area of the 3DMM material is in the range of 20-1000 m.sup.2/g. The 3DMM material array can be used for rapid synthesis, screening and manufacture of catalysts and nanosensors.

There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, on device assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

Method for manufacturing microarrays and verifying the quality of said microarrays, wherein the method comprises: a) providing at least one reagent, b) loading said at least one reagent in a dispensing print head, in a predetermined arrangement, c) in a first print pass, generating instructions for the print head and moving said print head with respect to a substrate to print said at least one reagent on the substrate to obtain microarrays, d) obtaining an image of the printed microarrays by means of a camera, e) processing the obtained images of the printed microarrays, to calculate parameters indicative for the quality of the printed microarrays, f) comparing, at the end of the first print pass, the calculated parameters for the printed microarrays with predetermined criteria for the microarrays, to identify possible printing defects, g) comparing, for the printed microarrays, the identified printing defects of step f), h) using the outcome of the comparison of step g) to select a corrective action to improve the quality of the microarrays, prior to the printing of a subsequent print pass.

A flow cell and cartridge assembly may be loaded into a processing system, such as for genetic sequencing. The system locates the assembly and is then actuated to move the assembly to a desired reference position in both X- and Y-directions. Further actuation causes clamps to contact the flow cell, the cartridge, or both to exert a hold-down force during processing. Further hold-down forces may be provided by a vacuum chuck. Fluid connections are also made by manifolds that contact the flow cell. The hold-down forces counteract the forces needed for sealing the manifolds to the flow cell.

Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, on device assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.

Embodiments are directed towards methods and systems of depositing a uniform test-pathogen mixture onto a test article for testing the sterilization efficacy of an electromagnetic radiation or other sterilization process. The system includes a holding mechanism configured to removably secure the test article to the system. The system also includes a test-pathogen dispenser configured to uniformly deposit the test-pathogen mixture onto a reference surface of the test article. The system is structured so that at least one of the test article and the test-pathogen dispenser moves relative to the other. A plurality of test-pathogen mixture droplets or lines is deposited onto the reference surface in a predetermined test-pathogen pattern, such as, for example, a plurality of rows and columns of droplets. A distance from a dispenser tip of the test-pathogen dispenser to the reference surface of the test article may be determined to help maintain consistency between test-pathogen mixture droplets or lines.

A sample loading plate that includes at least one sample mounting spot that mount a sample thereon is provided with a substrate having a conductive surface and an insulating film that is laminated on the conductive surface of the substrate and that has at least an insulating surface, the insulating film being sparsely formed so that the conductive surface of the substrate is partially exposed at least in the sample mounting spot. Thus, a voltage applied to the sample loading plate can effectively place the sample in an electric field. As a result of which, in a mass spectrometric analysis of the sample, there is no charge up of the sample and appropriate ionization becomes possible.

Apparatus and methods are disclosed for electrically active combinatorial-chemical (EACC) chips for biochemical analyte detection. An apparatus includes a substrate that has an array of regions defining multiple cells, wherein each of the cells includes a reaction cavity that contains multiple functional binding groups. A method of detecting an analyte providing the reaction cavity between a source and a drain or a pair of electrodes, applying a voltage and monitoring a parameter indicative of an analyte characteristic. A process of fabricating an EACC include bonding an analyte to the multiple functional binding groups of each reaction cavity, and forming an analyte sensing structure including the substrate.