B29C65/484

System and method for processing and detecting nucleic acids

A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids.

HYRBID WELDING OF THERMOPLASTICS
20180117852 · 2018-05-03 ·

A process for welding of at least two separate vehicle panels (12, 14) to form an integral part (16) for a vehicle. There is provided at least one first panel (12) including a first weld line area (18) and at least one second panel (14) including a corresponding second weld line area (19), the second weld line area (19) abutting in a weldable relationship to the first weld line area (18). The first and second weld line areas (18, 19) have at least one first weldable portion (22) and at least one second portion (24) at which welding is substantially hindered during welding attachment of the first and second panels (12, 14). The process includes applying a curable adhesive at each second portion (24), placing in a welding fixture and welding the panels (12, 14) at each first weldable portion (22) such that the panels (12, 14) are held together during curing of the adhesive.

MICROFLUIDIC CARTRIDGE FOR PROCESSING AND DETECTING NUCLEIC ACIDS

A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

Apparatus and Method to Repair the Junction of a Sewer Main Line and Lateral Pipe
20180080593 · 2018-03-22 · ·

A liner assembly and method for lining a damaged pipe junction between a main and lateral pipe is provided. The liner assembly includes a main liner member and a lateral liner tube. A collar disposed near the juncture between the main liner member and lateral liner tube is impregnated with a liquid hydrophobic or hydrophilic material capable of curing in a flexible or rigid state with the material expanding in the presence of water. The hydrophobic or hydrophilic material can also be applied without the use of the collar. When the liner assembly is pressed against the main and lateral pipes, a portion of the hydrophilic or hydrophobic material is forced into the damaged pipe junction.

MICROFLUIDIC CARTRIDGE FOR PROCESSING AND DETECTING NUCLEIC ACIDS

A system, configured to facilitate processing and detection of nucleic acids, the system comprising a process fluid container and a cartridge comprising: a top layer, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a detection chamber, comprises a portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

METHODS OF JOINING COMPONENTS IN VEHICLE ASSEMBLIES

Methods of joining components to form vehicle assemblies, such as engine assemblies, are provided. The methods include arranging a first component having a first channel defined therein in a mold, arranging a second component having a second channel defined therein in the mold, and aligning the first and second channel to define a pin-receiving channel. At least one polymeric composite pin is inserted into the pin-receiving channel thereby joining the first and second components, wherein an adhesive is disposed adjacent to at least a portion of the polymeric composite pin.

FLUID LEAK REPAIR
20180023747 · 2018-01-25 ·

A fluid leak repair kit, comprising: a putty; a self-amalgamating tape; and a composite material for use in encompassing the self-amalgamating tape, said composite material comprising a flexible sheet or tape and a matrix component for binding the flexible sheet or tape, the matrix component comprising a resin or water-activated matrix component which, when cured, forms an outer shell.

Microfluidic cartridge for processing and detecting nucleic acids

A microfluidic cartridge, configured to facilitate processing and detection of nucleic acids, comprising: a top layer comprising a set of cartridge-aligning indentations, a set of sample port-reagent port pairs, a shared fluid port, a vent region, a heating region, and a set of Detection chambers; an intermediate substrate, coupled to the top layer comprising a waste chamber; an elastomeric layer, partially situated on the intermediate substrate; and a set of fluidic pathways, each formed by at least a portion of the top layer and a portion of the elastomeric layer, wherein each fluidic pathway is fluidically coupled to a sample port-reagent port pair, the shared fluid port, and a Detection chamber, comprises a turnabout portion passing through the heating region, and is configured to be occluded upon deformation of the elastomeric layer, to transfer a waste fluid to the waste chamber, and to pass through the vent region.

GLOVE WITH SLEEVE AND PRODUCTION METHOD OF THE SAME
20170172233 · 2017-06-22 · ·

A glove with a sleeve is provided in which the bonding strength between the glove main body and the sleeve is great, allowing easy wearing and removal as well as easy overlapping of the glove main body and the sleeve upon bonding. The glove with a sleeve includes: a glove main body compromising rubber or a resin as a principal component; and a cylindrical sleeve comprising a thermoplastic resin as a principal component that is arranged to protrude from a cuff portion of the glove main body, in which the cuff portion of the glove main body is bonded to one end portion of the sleeve through a moisture-curing urethane-based hot melt adhesive. The moisture-curing urethane-based hot melt adhesive is preferably crosslinked. A post-curing thermal deformation temperature of the moisture-curing urethane-based hot melt adhesive is preferably no less than 80 C.

Functional element for attachment to a plastic component and a component assembly

A functional element of metal having a flange of larger diameter from an attachment surface and a centering section arranged inside the attachment surface and extending away from the flange, characterized in that an adhesive is arranged around the cylindrical section and adjacent to the attachment surface.