A method for purifying a mixture in a multicolumn chromatography system. The method successively and cyclically collects a raffinate, injects the mixture to be separated, collects an extract, and injects eluent. The mixture to be separated contains fructose and has a dry matter mass concentration of 45 to 55%. The method is carried out at a temperature of 50 to 62° C.

A single-use device for separating or purifying a large volume of a mixture of substances including membrane chromatography modules which are fixedly mounted in a predetermined grid and a line system for linking the membrane chromatography modules and for connecting the membrane chromatography modules to each other. A cover or bottom mechanism holding the membrane chromatography modules in position in a predetermined grid may be attached to the upper or lower side of the membrane chromatography modules. At least part of the line system is formed in the cover or the bottom mechanism, with connecting lines between the membrane chromatography modules. In a method of separating or purifying a large volume of a mixture of substances using such a single-use device having a plurality of automated valves and sensors connected to a control unit, the automated valves are controlled based on an evaluation of the parameters measured by the sensors.

A system for producing steam includes a source of superheated water with superheated water output; a membrane filtration system in fluid communication with the superheated water output and including a membrane filter with a permeate side and an opposing retentate side. The membrane filter includes a separation membrane constructed to reject organic molecules. The system may be used for removing organic compounds, such as anti-corrosion agents or contaminants, from superheated water to produce steam. A method for producing steam includes directing a cross-flow of heated pressurized water including a first concentration of an organic compound across a membrane filter. The membrane filter includes a separation membrane constructed to reject the organic compound; and one or more support layers adjacent the separation membrane. A steam permeate including a second concentration of the organic compound is collected, where the second concentration is lower than the first.

The present invention relates to a method for separating one or more components from a liquid feed mixture in an EBA-SMB operating mode without the need of pumps at the outlets of the EBA columns. The present invention also relates to a simulated moving bed separation device with expanded bed adsorption columns which can be used in the method according to the invention.

An optimization method for capturing proteins by multi-column continuous chromatography (MCC), including the following steps: step 1, under the conditions of a set loading protein concentration and an arbitrary load residence time, performing a single time of protein breakthrough experiment to obtain a protein breakthrough curve; step 2, under a set breakthrough percentage for a target protein, integrating the breakthrough curve to obtain a single-column loading capacity and establishing a linear relationship between the interconnected load time and the load residence time; step 3, solving for the optimal number of operating columns for capturing proteins by MCC based on step 2; step 4, solving for the optimal load residence time for capturing proteins by MCC based on step 2, step 3; and step 5, solving for the maximum productivity of capturing proteins by MCC based on step 4.

The present disclosure provides a macroporous noble metal catalyst and processes employing such catalysts for the regeneration of deactivated ionic liquid catalyst containing conjunct polymer.

Certain embodiments of the invention provides a method for monitoring level of saturation of a chromatography media in a column, which method comprises measuring a first pressure at the inlet of an unloaded column; measuring a second pressure at the inlet from a loaded column; and comparing the first and second pressure measurement to determine the level of saturation of the chromatography media. Embodiments of the invention also provide related methods for controlling a chromatography system and methods for controlling a periodic counter current chromatography system, as well as a chromatography system suitable for use with the novel methods.

Integral chromatography unit having an inlet and an outlet, and comprising one or more membranes interposable in the internal volume of the unit between the inlet and outlet. In certain embodiments, each of the membranes is allotted adequate space within the unit to swell by the placement of one or more spacers. Fluid entering the unit through a fluid inlet passes the membrane(s) and spacer(s) prior to exiting the unit through a fluid outlet.

The present disclosure is directed to methods and systems of purifying solvents. The purified solvents can be used for cleaning a semiconductor substrate in a multistep semiconductor manufacturing process.

Methods for achieving complete sequence coverage of monoclonal antibodies by trypsin digestion and dual-column LC-MS system are provided. The disclosed method improves upon current techniques for standard peptide mapping.