A method for reducing sequencing by synthesis cycle time using a microfluidic device is provided. The microfluidic device comprises a flow cell having an inlet port, an outlet port, and a flow channel extending between the inlet port and the outlet port, wherein the flow channel receives an analyte of interest and one or more reagents for analyzing and detecting molecules. To aid in the acceleration of the reactions, the microfluidic device comprises a mixing device to increase the rates of diffusion of the reagents from the fluid bulk to an active surface of the flow cell. The mixing device comprises at least one of an electrothermal mixing device, an active mechanical mixing device, and a vibrational mixing device.

This disclosure relates generally to mixing and separating phases of a mixture, and, more particularly, to an integrated apparatus for mixing and separating immiscible fluid phases and method therefor. The apparatus includes an inlet section, a mixing section, a separating section, and an outlet section. The mixing section includes multiple spiral turns for separately introducing the fluid phases of different specific gravity into the mixing section. Each of the spiral turns includes a helical channel followed by a counter-helical channel for enabling mixing of fluid phases. The counter-helical channel changes the direction of flow of fluid phases upon flow of said fluid phases from the helical channel to the counter-helical channel. The separating section extends from the mixing section separates the fluid phases based on specific gravity difference of fluid phases. The outlet section facilitates in separate withdrawal of fluid phases based on the specific gravity of said fluid phases.

An exhaust gas recirculation ejector system for an engine that includes an air conduit coupled to an engine providing charge air to the engine. The air conduit includes at least one bend formed therein. The at least one bend includes a port formed therein. An EGR conduit is coupled to an exhaust manifold of the engine at a first end of the EGR conduit. A second end of the EGR conduit passes through the port and extends into the air conduit at the bend defining an ejector mixing the charge air and exhaust gas before entry into the engine.

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

Provided is a nano-micro bubble generator according to one aspect of the present invention, the nano-micro bubble generator including: a housing which a fluid flows into and out of; a plurality of rotors rotatably coupled to the inside of the housing; and a plurality of stators fixed to the inside of the housing and alternately arranged with the plurality of rotors, wherein at least one of the rotors and the stators has a mesh-like structure in which a plurality of flow passages of the fluid are arranged in a lattice form, and the rotors and the stators are arranged to be adjacent to each other so as to generate a collision, friction, and cavitation due to rotation of the rotors in the fluid flowing through the flow passages, thereby generating at least one of nano bubbles and micro bubbles in the fluid.

A solvent mixing system includes a mixing tee, a centrifugal mixing path, and a low frequency blending mixer. The mixing tee has at least two solvent input ports and a solvent output port in fluid communication with one another. The centrifugal mixing path has a mixing path inlet in fluid communication with the solvent output port of the mixing tee. The centrifugal mixing path includes at least one coiled segment between the mixing path inlet and a mixing path outlet. The low frequency blending mixer is in fluid communication with the outlet of the centrifugal mixing path.

A method for preparing droplets using a microfluidic chip system is provided. The microfluidic chip system includes a droplet generation device, a power generation device, a collection bottle, a connection device, and a preparation platform, the droplet generation device includes a chip body, the chip body defines a continuous phase inlet for receiving a continuous phase and a dispersed phase inlet for receiving a dispersed phase. The power generation device is activated to form a pressure difference among the collection bottle, the connection device, and the chip body, wherein the pressure difference promotes the dispersed phase and the continuous phase to converge and flow into the collection bottle in form of the droplets.

A medicament preparation system includes a disposable cartridge with a flow path. Various sensors may be placed on the cartridge to measure qualities of the fluid flowing through the flow path. The sensors are placed in precise locations using various approaches that make manufacturing of the cartridge efficient and repeatable. A drain line that is susceptible to fouling may be preattached and various approaches are used to remove or reduce the fouling. An elastomeric contact can also be present in the medical preparation system and used in a conductivity measurement subsystem.

Exemplary modular gas delivery assemblies may include a plurality of modular gas blocks coupled together. Each gas block may include an upper portion and a lower portion. A first end of the upper portion may extend beyond a first end of the lower portion and a second end of the lower portion may extend beyond a second end of the upper portion. A first fluid channel may include a first fluid port, a second fluid port, and a third fluid port. The block body may define a second fluid channel that extends transversely to the first fluid channel. A first modular gas block may be coupled with a second modular gas block and a third modular gas block such that the first fluid channels of each of the first, second, and third modular gas blocks are fluidly coupled with one another.

The application relates to microfluidic apparatus and methods of use thereof. Provided in one example is a microfluidic device comprising: a first fluidic input and a second fluidic input; and a fluidic intersection channel to receive fluid from the first fluidic input and the second fluidic input, wherein the fluidic intersection channel opens into a first mixing chamber on an upper region of a first side of the first mixing chamber, wherein the first mixing chamber has a length, a width, and a depth, wherein the depth is greater than about 1.5 times a depth of the fluidic intersection channel; an outlet channel on an upper region of a second side of the first mixing chamber, wherein the outlet channel has a depth that is less than the depth of the first mixing chamber, and wherein an opening of the outlet channel is offset along a width of the second side of the first mixing chamber relative to the fluidic intersection.