In some aspects, the present disclosure pertains to sample treatment methods that comprise: contacting an acidic elution solution that is free of primary amine, secondary amine and thiol groups with a sorbent having bound target antibody and separating the elution solution from the sorbent, thereby releasing bound target antibody from the sorbent and forming a first collection fraction that comprises the elution solution and released target antibody; contacting the sorbent with a neutralization buffer solution that is free of primary amine, secondary amine and thiol groups and separating the neutralization buffer solution from the sorbent, thereby forming a second collection fraction that comprises the neutralization buffer solution; and forming a neutralized solution that comprises the first collection fraction and the second collection fraction. In other aspects, the present disclosure pertains to kits for performing such sample treatment methods.

The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.

A column for removal of a component from a fluid is disclosed. The column has a compartment with a cross sectional area. The compartment contains beads having a diameter. A ligand selected to bind to the component is coupled to the beads. The cross-sectional area and bead diameter are selected to maintain a flow velocity of the fluid within the compartment below a first threshold, thereby reducing leaching of the ligand into the fluid. Also described herein is an adsorbent comprising a ligand that is attached to a substrate by an amine bond, wherein the ligand is resistant to dissociation from the substrate.

A chromatography carrier capable of removing an antibody dimer from a solution containing an antibody monomer. The chromatography carrier includes a base carrier containing porous particles and a hydrophobic ligand bound to the base carrier, and has an electric conductivity of 34 mS/cm or less measured by a gradient elution test. The porous particles preferably have an average particle diameter of 66 to 150 μm, and the hydrophobic ligand preferably has at least one selected from a group consisting of phenyl, n-butyl, n-hexyl, n-octyl, and n-octadecyl.

Provided is a method for preparing a superabsorbent polymer. More particularly, provided is a method for preparing a superabsorbent polymer having a reduced amount of coarse particles with a particle size of more than 850 μm and an improved water content, and exhibiting excellent absorption performances without deviation.

The present invention pertains to present invention relates to a device comprising conjugate/s, and uses thereof in depleting at least one amine, specifically ammonia from body fluids. The present disclosure further provides systems, apparatus, conjugates, plurality of conjugates, and methods. More specifically, the conjugate comprising a particle bonded to at least one linker comprising a chain of n carbon atoms covalently bonded to m carbonyl groups, and at least one trapping agent A covalently bonded to the m.sup.th carbonyl group, (Formula I) wherein, n is an integer within the range of 5 to 15, and m is an integer within the range of 5 to 10, wherein trapping agent A is characterized by having the ability to capture or bind amine. In some optional embodiments, the amine is at least one of methylamine, dimethylamine or trimethylamine. In some embodiments, the linker of the conjugate of the disclosed device comprises a straight chain alkane and m carbonyl groups

The invention relates to superabsorbent polymer that not only has excellent basic absorption performance, but also exhibits more improved permeability under pressure, and thus, can improve rewet property and leak inhibition property of hygienic products such as a diaper, and the like, and a method for preparing the same. The superabsorbent polymer comprises base resin powder comprising first crosslinked polymer of water soluble ethylenically unsaturated monomers having acid groups of which at least a part are neutralized; and a surface crosslink layer on the base resin powder, comprising second crosslinked polymer formed by additional crosslinking of the first crosslinked polymer by a surface crosslinking agent, wherein the surface crosslinking agent comprises a polymer type first surface crosslinking agent having number average molecular weight of 300 or more, and having plural hydroxy groups or epoxy groups.

The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Novel chromatographic materials for chromatographic separations, columns, kits, and methods for preparation and separations with a superficially porous material comprising a substantially nonporous core and one or more layers of a porous shell material surrounding the core. The material of the invention is comprised of superficially porous particles and a narrow particle size distrution. The material of the invention is comprised of a superficially porous monolith, the substantially nonporous core material is silica; silica coated with an inorganic/organic hybrid surrounding materia; a magnetic core material; a magnetic core material coated with silica; a high thermal conductivity core material; a high thermal conductivity core material coated with silica; a composite material; an inorganic/organic hybrid surrounding material; a composite material coated with silica; a magnetic core material coated with an inorganic/organic hybrid surrounding material; or a high thermal conductivity core material coated with an inorganic/organic hybrid surrounding material.