Provided is a manufacturing method for monolithic structure that is a porous body formed of polysaccharide being a naturally-occurring polymer, has continuous pores with an average pore diameter suitable for biomolecule separation, and allows formation into arbitrary shape. The polysaccharide monolithic structure is manufactured by a method including a first step of obtaining a polysaccharide solution by dissolving polysaccharide into a mixed solvent of a first component and a second component at temperature lower than a boiling point of the mixed solvent, and a second step of obtaining polysaccharide monolithic structure by cooling the polysaccharide solution, wherein the first component is a solvent selected from lactate, and the second component is a solvent selected from water, lower alcohol, and a combination thereof. The monolithic structure obtained is a porous body having continuous pores with an average pore diameter of 0.01 to 20.0 micrometers, and a thickness of 100 micrometers or more.

An object of the present invention is to provide an adsorbent material having high dispersibility and reversibility. The adsorbent material has a polymer material having a plurality of functional groups ionizable in water and exhibiting no lower limit critical solution temperature, an adsorption site capable of interacting with a target substance, and a carrier.

The present invention relates to a filtering device (8) for removing substances from blood or a blood component, the filtering device (8) comprising: a housing having an inlet and an outlet, at first sorbent material coupled with at least a first ligand located within the housing, and a second sorbent material coupled with at least a second ligand located within the housing, wherein the first ligand is for removing free hemoglobin (fHb) and the second ligand is for removing microvesicles (MV) from the blood or blood component passing through the filtering device (8), from the inlet to the outlet, wherein the first and second ligand are different from each other, and wherein the first and second sorbent material are the same or are different from each other.

Disclosed is a separation material comprising: a porous polymer particle containing a crosslinked polymer containing a structural unit derived from a crosslinkable monomer having an aromatic group and two or more vinyl groups bonded to the aromatic group; and a coating layer coating at least part of the surface of the porous polymer. The coating layer contains a first graft chain that is a polymer having a hydroxyl group bonded to the crosslinked polymer, and a second graft chain that is a polymer having a hydroxyl group, bonded to the first graft chain, and being different from the first graft chain.

A disposable, virus-trapping membrane, and a corresponding method to remove viruses from solution are described. The membrane includes a disposable, micro-porous filter membrane and a ligand immobilized on the membrane. The ligand irreversibly and selectively binds viruses. The ligand also has a pKa sufficiently high to repel antibodies via electrostatic charge repulsion.

Described herein are passive samplers, making of such samplers, and methods of use. In an example embodiment, a passive sampling membrane comprises, for example, a continuous mesoporous sequestration media having a sequestration phase and a support membrane configured to support the sequestration phase. The sequestration phase may include a hydrophobic region and a hydrophilic region. The continuous mesoporous sequestration media may be configured to simultaneously sequester polar and non-polar organic substances.

A synthetic polymeric porous medium with a core-shell(s) hierarchical layer structure and has an essentially homogeneous porous structure from inside to outside of the medium, whose core and shell(s) are covalently modified with distinct chemical functional groups or same functional group with different density. Here the methodologies for resin syntheses and core-shell(s) modifications and liquid chromatographic applications of the newly developed resins in the field of analysis and purification of Tween surfactants, virus-like particles (VLP)/vaccines/viral vectors/viruses, antibody, and mRNA are disclosed.

Methods of using a separation media to isolate a target molecule that includes a carbohydrate are disclosed. The separation media includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity capable of recognizing and binding to a carbohydrate.

Separation media that includes a support substrate and a plurality of separation ligands immobilized on the support substrate. The plurality of separation ligands include an affinity capable of recognizing and binding to blood type antigen recognizing domain. Methos of making the separation media and methods of using the separation media.

The present invention provides novel chromatographic materials. e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The chromatographic materials of the invention have controlled porosity and comprise a chromatographic core material and one or more layers of chromatographic surface material which each independently provide an average pore diameter, an average pore volume, or a specific surface area such that the combined layers form a chromatographic material having a predetermined or desired pattern of porosity from the core material to the outermost surface. The materials are useful for HPLC separations, normal-phase selarations, reversed-phase separations, chiral separations, HILIC separations, SFC separations, affinity separations, perfusive separations, partially perfusive separations, and SEC separations.