A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites.

A device for parallel oligomer synthesis having a centrifuge with a plurality of reactor holders configured to retain reactors at an angle and a plurality of siphon based outflow holders are disclosed. A method of parallel solid-based peptide synthesis following the timing protocol of the device and a use of the device for parallel oligomer synthesis are also disclosed.

A microreactor array platform and method for sealing a reagent in microreactors of an array of microreactors are provided. The microreactor array platform includes an array of microreactors, and a sealing film having a first surface and an opposite second surface, the sealing film configured to movably seal the array of microreactors. The microreactor array platform also includes an injector for delivering a reagent into the array of microreactors via a fluid path between the array and the second surface of the sealing film, and an applicator for directing a sealing liquid against the first surface of the sealing film. The microreactor array platform further includes a system for creating a pressure differential between the reagent in the injector and a space between the array of microreactors and the second surface of the sealing film.

The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.

The present invention relates to a method of catalytic process characterization which comprises a reaction system having two or more reaction strands in a parallel arrangement, wherein an individual reaction strand comprises multiple series-connected reaction chambers or a single reaction chamber. In the method, which is also referred to as CPC method, each reaction strand is supplied with a reactant stream. The reactant streams supplied to the reaction strands are subjected to different numbers of process stages in the different reaction strands. The product streams discharged from the reaction strands are subjected to an analytical characterization, wherein the data achieved in the characterization are expressed in relative terms, here preferably including the forming of a difference. The CPC method can be used in a very versatile manner and is characterized by very high accuracy. The mass balance achieves a standard deviation of +/10% by weight or lower. Furthermore, the invention relates to an apparatus for performing the CPC method or else to an apparatus for simultaneously performing a multitude of CPC methods. The invention thus also relates to the field of high-throughput research.

A method of synthesizing an oligonucleotide using a nanofluidic device including a plurality of nanopore channels, a plurality of electrodes, and an electrolyte solution, includes coupling a primer to an inner wall of a nanopore channel of the plurality of nanopore channels, the primer having a protecting group. The method also includes applying a voltage to an electrode of the plurality of electrodes that corresponds to the nanopore channel to produce an acid from the electrolyte solution at the electrode. The electrode includes an anode and a cathode disposed at opposite sides of the nanopore channel. The method further includes the acid removing the protecting group from the primer. Moreover, the method includes coupling a nucleotide to the primer with the protecting group removed to form an intermediate product. In addition, the method includes repeating the steps on the intermediate product until the oligonucleotide is synthesized.

The present disclosure is directed to a device (100) comprising a sampling part (110), wherein the sampling part (110) comprises an array of capture zones (112) for sampling liquid volumes between tens of microliters and femtoliters, wherein some or all of the capture zones (112) contain a sponge-like material. Also disclosed are a method for the preparation of such a device and a method for the detection and determination of the presence, concentration and/or properties of an analyte by contacting a liquid sample with such a device.

An example method includes reacting a first solution and a different, second solution on a flow cell by flowing the first solution over amplification sites on the flow cell and subsequently flowing the second solution over the amplification sites. The first solution includes target nucleic acids and a first reagent mixture that comprises nucleoside triphosphates and replication enzymes. The target nucleic acids in the first solution transport to and bind to the amplification sites at a transport rate. The first reagent mixture amplifies the target nucleic acids that are bound to the amplification sites to produce clonal populations of amplicons originating from corresponding target nucleic acids. The amplicons are produced at an amplification rate that exceeds the transport rate. The second solution includes a second reagent mixture and lacks the target nucleic acids. The second solution is to increase a number of the amplicons at the amplification sites.

A flow cell and cartridge assembly may be loaded into a processing system, such as for genetic sequencing. The system locates the assembly and is then actuated to move the assembly to a desired reference position in both X- and Y-directions. Further actuation causes clamps to contact the flow cell, the cartridge, or both to exert a hold-down force during processing. Further hold-down forces may be provided by a vacuum chuck. Fluid connections are also made by manifolds that contact the flow cell. The hold-down forces counteract the forces needed for sealing the manifolds to the flow cell.