Disclosed are fish with impaired reproductive capacity and methods and compositions for producing the same. The reproductively impaired fish may include catfish, such as Ictalurus punctatus. The disclosed methods may be generally useful for mitigating environmental impact of escaped genetically engineered fish.

The transplant fish production method includes selecting a donor fish species, selecting first and second fish species which are a combination that could be sterile, producing a hybrid fish species of the selected first fish species and second fish species, and transplanting reproductive cells of the donor fish species into the produced hybrid fish species.

In alternative embodiments, the invention provides nucleic acid sequences that are genetic polymorphic variations of the human TMEM216 gene, and TMEM216 polypeptide encoded by these variant alleles. In alternative embodiments, the invention provides methods of determining or predicting a predisposition to, or the presence of, a ciliopathy (or any genetic disorder of a cellular cilia or cilia anchoring structure, basal body or ciliary function) in an individual, such as a Joubert Syndrome (JS), a Joubert Syndrome Related Disorder (JSRD) or a Meckel Syndrome (MKS). In alternative embodiments, the invention provides compositions and methods for the identification of genetic polymorphic variations in the human TMEM216 gene, and methods of using the identified genetic polymorphisms and the proteins they encode, e.g., to screen for compounds that can modulate the human TMEM216 gene product, and possibly treat JS, JSRD or MKS. In alternative embodiments, the invention provides cells, cell lines and/or non-human transgenic animals that can be used as screening or model systems for studying ciliopathies and testing various therapeutic approaches in treating ciliopathies, e.g., JS, JSRD or MKS.

Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.

A behavior modification device includes processing circuitry configured to: acquire sensing information of a sensor to observe a monitoring area; identify behavior of an organism to be threatened present in the monitoring area on a basis of the acquired sensing information; determine a parameter of a stimulus to be given to the organism on a basis of the identified behavior; and generate stimulus data for generating the stimulus on a basis of the determined parameter and output the stimulus data to an actuator for generating a stimulus.

The subject invention provides microbe-based products, as well as their use, for enhancing aquaculture. In particular, the subject invention provides materials and methods for producing healthy and high quality farmed fish by applying yeast and/or biosurfactant-based compositions to a fish farm. In one embodiment, the subject invention improves the environment in which the fish are farmed. In another embodiment, the subject invention provides a highly nutritious fish feed, as well as methods of feeding fish at low cost.

The invention relates to methods of treating an individual suffering from a structural cardiac muscle defect, comprising providing cardiomyocytes of at least part of the cardiac muscle of the individual with a high mobility group A (HMGA) protein to thereby promote proliferation of said cardiomyocytes. The invention further relates to an expression construct for functional expression of said HMGA protein, a pharmaceutical composition, comprising HMGA or said expression construct, and to a method of culturing cardiomyocytes in vitro, comprising providing cardiomyocytes with a HMGA protein or the expression construct.

Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature. One embodiment includes compositions and methods for making livestock with a polled allele, including migrating a polled allele into a bovine species without changing other genes or chromosomal portions.

The present disclosure relates to transgenic ornamental fish, as well as methods of making such fish by germ cell transplantation techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing, distributing and selling such transgenic fish.

Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.