Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.

The present invention relates to transgenic red ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.

Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.

A display apparatus includes a transparent electronic display such as a non-backlit LCD screen, a display area disposed behind the transparent electronic display, and a light source for illuminating the display area. When the light source is activated, the pixels of the LCD screen, and objects behind the screen, become visible. Various examples of the display apparatus include additional enhancements to the visual image and/or functionality. These enhancements include additional monitors for coordinated displays, user-controlled turntables with coordinated informational displays, parallax compensation, RFID sensors for identifying objects to be displayed, and switchable liquid crystal films and/or polarized mirror coatings to further control revealing and concealing of objects behind the screen. Some examples of the display apparatus may include an animal habitat. The animal habitat may be selectively revealed or concealed. The display apparatus may incorporate screen displays with animal movements for unique visual effects.

Protein indicators useful for calcium imaging, in particular, red genetically-encoded calcium indicators (GECIs) disclosed herein rival best-of-class green GECIs in terms of sensitivity for detecting neural activity, and can be monitored in vivo. The presently-disclosed subject matter further includes a method of monitoring cell activity comprising stimulating a cell comprising a red GECI polypeptide; and detecting fluorescence emitted by the cell.

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by m vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

The present invention relates to transgenic ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.

Among other things, the present disclosure provides USH2A oligonucleotides, and compositions and methods of use thereof, for preventing and/or treating various conditions, disorders or diseases. In some embodiments, provided USH2A oligonucleotides comprise nucleobase modifications, sugar modifications, internucleotidic linkage modifications and/or patterns thereof, and have improved properties, activities and/or selectivities. In some embodiments, the present disclosure provides USH2A oligonucleotides, compositions and methods for preventing and/or treating USH2A-related conditions, disorders or diseases, such as Usher Syndrome (e.g., Usher Syndrome Type 2A), atypical Usher syndrome, or nonsyndromic retinitis pigmentosa.

A decoupled aquaponic system having two loop subsystems: hydroponic (Hp-loop) (2) and aquaculture (RAS) (1) interconnected by a third loop subsystem performing reverse osmosis filtration (OI) of RAS (1) recirculation water to achieve a higher nutrient quantity in the former and higher quality recycled water in the latter, with consequent energy savings due to its variable control structure and OI membrane effectiveness. Prior to entering the system containing the OI membrane, a water ultrafiltration (UF) (22) treatment is added to preserve its lifespan.