A reel, such as a spincast reel, provides increased cranking power by eliminating sliding contact associated with a line pickup mechanism when the reel is in line retrieval mode. Fishing line is retrieved by locating a cam member proximate a body spool boss during a casting state for positioning the cam member to be acted on by cam engaging structure of the body spool boss. The cam member is rotated relative to the body spool boss. The cam member and a pick up pin are moved radially outwardly by the cam engaging structure. The cam member is axially moved away from the body spool boss. The cam member may then be rotated relative to the body spool boss during a line retrieve state, wherein the cam member and the body spool boss are spaced apart from one another for facilitating frictionless relative rotation.

A mount including a base and a handle. The base is capable of releasably coupling to a pole, for example outrigger poles, fishing rods, and shade poles. A handle of the mount can be loosened to allow the base to be rotated to position the pole in a desired extended direction. When the handle is tightened, the base is restricted from rotating.

Methods, uses, and compositions for manipulating genomic DNA. Some of the embodiments of the invention provide for making a founder animal that is completely free of all unplanned genetic modifications. Some embodiments are directed to removing genetic faults in established breeds without making other alterations to the genome. Other embodiments are directed to particular tools or processes such as TALENs or CRISPR with a preferred truncation. One embodiment involves introducing a targeted targeting endonuclease system and a HDR template into a cell (optionally with a mismatch in the binding of the targeting endonuclease and the targeted site). Another embodiment includes processes of making a genetically modified livestock animal comprising a genome that comprises inactivation of a neuroendocrine gene selective for sexual maturation, with the inactivation of the gene preventing the animal from becoming sexually mature. One embodiment includes compositions and methods for making livestock with a polled allele, including migrating a polled allele into a bovine species without changing other genes or chromosomal portions.

A fish of a tilapia genus comprising a loss-of-function mutation in a slc45a2 gene, wherein the mutation is in a homozygous form, and wherein the loss-of-function mutation results in an albino-red phenotype of the fish is disclosed. Methods of generating the fish are also disclosed.

Disclosed are non-naturally occurring zebrafish, such as transgenic zebrafish, which comprise a mutation in the rhodopsin (rho) gene. Also disclosed are methods of identifying compounds useful in treating retinal-specific defects and disorders, such as degeneration. Further disclosed are methods of identifying mutations in the rhodopsin gene that can cause retinal-specific defects.

Disclosed are non-naturally occurring zebrafish, such as transgenic zebrafish, which comprise a mutation in the rhodopsin (rho) gene. Also disclosed are methods of identifying compounds useful in treating retinal-specific defects and disorders, such as degeneration. Further disclosed are methods of identifying mutations in the rhodopsin gene that can cause retinal-specific defects.

Methods, systems, and apparatuses, including computer programs encoded on a computer-readable storage medium for fish farm material handling are described. Fish farm material includes samples collected from broodstock individuals and transported for suspension in cold storage. During the time window of cold storage, a metric of interest corresponding to the fish farm material is identified. Based on the metric of interest identification, material handling may be modified to, for example, segregate fish farm material into quarantined incubation units to inhibit infection of fish farm material within other incubation units.

A circulating water purification system for aquaculture breeding, including a feeding machine, a growing pond, a water purification and separation system, and a recycling device, where the feeding machine for feeding fry to the growing pond is disposed outside the growing pond, the upper part of the growing pond is provided with a water-level safety line, the bottom of the growing pond is conical, and a mesh for filtering solids from liquids are mounted at the bottom of the growing pond; the bottom of the growing pond is connected to the water purification and separation system through a first discharge pipe; the water purification and separation system are provided with a feed pump, and pumps water to the growing pond through the feed pump; and the water purification and separation system are connected to the recycling device.

The present invention provides DNAzymes for reduction in the expression of the target gene implicated in disease. The present invention also provides a platform technology for the use of DNAzymes to create a reduction in gene expression in zebrafish embryos. More particularly, the present invention describes DNAzyme mediated knockdown of Fmr1 mRNA, gcdh mRNA and gba1mRNA in zebrafish embryos. The present invention also provides a method of creating a model of Fragile X syndrome in Zebrafish embryos.

In alternative embodiments, the invention provides nucleic acid sequences that are genetic polymorphic variations of the human TMEM216 gene, and TMEM216 polypeptide encoded by these variant alleles. In alternative embodiments, the invention provides methods of determining or predicting a predisposition to, or the presence of, a ciliopathy (or any genetic disorder of a. cellular cilia or cilia anchoring structure, basal body or ciliary function) in an individual, such as a Joubert Syndrome (JS), a Joubert Syndrome Related Disorder (JSRD) or a Meckel Syndrome (MKS). In alternative embodiments, the invention provides compositions and methods for the identification of genetic polymorphic variations in the human TMEM216 gene, and methods of using the identified genetic polymorphisms and the proteins they encode, e.g., to screen for compounds that can modulate the human TMEM216 gene product, and possibly treat JS, JSRD or MKS. an alternative embodiments, the invention provides cells, cell lines and/or non-human transgenic animals that can be used as screening or model systems for studying ciliopathies and testing various therapeutic approaches in treating ciliopathies, e.g., JS, JSRD or MKS.