The present invention relates to stabilized probiotic compositions that include lactic-acid-producing bacteria. The present invention also relates to methods for stabilizing lactic-acid-producing bacteria with a diluent having a plurality of monosaccharide units connected predominantly by -glycosidic bonds or -glycosidic bonds that are resistant to hydrolysis by an amylase.

The present invention is directed to compositions comprising a mixture of microbes and the metabolites produced when the microbes are grown together. The invention is further directed to methods for making and using the compositions.

The present invention is directed to compositions comprising a mixture of microbes and the metabolites produced when the microbes are grown together. The invention is further directed to methods for making and using the compositions.

A bacterial composition and method that inhibits E. coli O157:H7 growth by as much as 93% and Salmonella growth by as much as 97%, together with commensurate inhibition rates against the Big-Six Escherichia coli strains referred to as the non-O157 STECs that include E. coli O121:H19; E. coli O45:H2; E. coli O103:H11; E. coli O145, E. coli O26:H11; and E. coli O111. The composition is constituted of various combinations of the following unique pathogen-inhibiting bacteria: (1) Lactobacillus animalis; (2) Enterococcus faecium (3) Pediococcus acidilactici.

The present teachings disclose a process for producing a safe and preserved food. The process includes: (i) introducing a non-live-bacteria preparation on or in a food to produce an activated food, and the non-live-bacteria preparation results from fermentation of fermented bacteria, but the non-live-bacteria preparation is substantially depleted of the fermented bacteria, and the fermented bacteria are bacteria that are living; and (ii) incubating the activated food in presence of the non-live-bacteria preparation to kill and/or inhibit growth of pathogens and/or spoilage microorganisms on and/or in the activated food and thereby producing the safe and preserved food; and wherein the fermented bacteria is anti-pathogen bacteria and/or anti-spoilage bacteria.

The invention discloses a Pediococcus acidilactici derived antimicrobial hexapeptide and a preparation method thereof and belongs to the field of bioengineering. The antimicrobial peptide ENGEEE is reported for the first time. Antimicrobial peptide lactein R16 has good pH stability and thermal stability, has an effect on inhibiting Escherichia coli, Listeria monocytogenes and Staphylococcus aureus and is capable of effectively reducing the amount of the Escherichia coli in soybean meal. Secondly, the antimicrobial peptide lactein R16 has a certain effect on proliferating Saccharomyces cerevisiae and Lactobacillus plantarum and has certain capability of clearing hydrogen peroxide, hydroxyl radicals, DPPH.radicals and superoxide anions. The antimicrobial peptide lactein R16 can be used for biological control and can be used as a feed additive to play important roles in substituting antibiotics and solving the feed safety problem.

A first livestock feed additive comprises calcium, phosphorus, sodium chloride, potassium, sulfur, magnesium, manganese, zinc, iron, copper, selenium, vitamin A, Vitamin E, and mendione; a second livestock feed additive comprising sodium bentonite, sodium sulfate, dried Aspergillus oryzae fermentation product, ferrous sulfate, calcium carbonate, manganese proteinate, manganese sulfate, molasses products, vegetable oil, lecithin, high fructose corn syrup, polysorbate 60, glycerin, sulfuric acid, xanthan gum, natural and artificial flavor, zinc proteinate, selenium yeast, zinc sulfate, yeast culture, vitamin A supplement, vitamin D-3 supplement, vitamin E supplement, choline chloride, niacin, calcium pantothenate, riboflavin, vitamin B-12 supplement, thiamine hydrochloride, copper sulfate, copper proteinate, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus plantarum, Enterococcus faecium, and Pediococcus acidlacticii; and a livestock drench composition comprising crude protein, crude fat, fiber, copper, manganese, zinc, vitamin E, phosphorus, sodium chloride, magnesium, potassium, iron, iodine, and sulfur.

The present invention relates to a method of reducing the concentration of Gram-negative bacteria in a food product, the method comprising adding a red wine extract produced from red wine by dealcoholization, concentration and drying, and a culture comprising at least one class IIa bacteriocin-producing lactic acid bacterial strain to a food product, ripening the food product, and storing the food product at a temperature of at the most 15 C. until a concentration less than 1 E-1 of Gram-negative bacteria. In a preferred embodiment of the invention, Gram-negative bacteria already present are eliminated. In one embodiment of the invention, the Gram-negative bacteria are Salmonella ssp. The bacteriocin-producing culture may comprise at least one of Lactobacillus curvatus (DSM 18775) and Pediococcus acidilactici (DSM 28307). The invention further relates to a kit for reducing the concentration of Gram-negative bacteria in a food product comprising a red wine extract produced from red wine by dealcoholization, concentration and drying, and a culture comprising at least one class IIa bacteriocin-producing lactic acid bacterial strain.

The present invention relates to pre-, pro-, and postbiotic compositions and methods of use for improving the health and nutrition of commercial livestock and companion pets.

The present disclosure is directed to methods of administering and compositions comprising Lactobacillus plantarum CECT 7478, Lactobacillus plantarum CECT 7485, and Pediococcus acidilactici CECT 7483 or a mutant or variant thereof. The compositions and methods are useful in the probiotic treatment of bowel diseases or conditions such as Inflammatory Bowel Disease, Irritable Bowel Syndrome, and abdominal distension and bloating.