An aerosol-generating article according to one aspect may include: a first segment including a first aerosol-generating material excluding nicotine; and a second segment including a second aerosol-generating material including the nicotine, wherein the first aerosol-generating material includes a moisturizing material in an amount of at least 30 mg by weight.

Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.

Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that negatively regulates alkaloid biosynthesis, such as nicotine biosynthesis.

An article for use with an apparatus for heating aerosol generating agent to volatilize at least one component of the aerosol generating agent, the article including a support layer having a first surface, wherein at least a portion of the first surface is rough and an aerosol generating agent on the portion of the first surface that is rough.

An aerosol-forming substrate is provided, including: a) one or both of cellulose and cellulose derivatives; b) an aerosol-former present in an amount of 20 weight percent to 58 percent on a dry weight basis based on a total amount of the aerosol-forming substrate; c) from 0 weight percent to 5 weight percent of tobacco on a dry weight basis based on the total amount of the aerosol-forming substrate; d) a nitrogen-containing nucleophilic compound; and e) a disaccharide. An aerosol-generating article including a substrate portion containing the aerosol-forming substrate is also provided. An aerosol-generating system is also provided.

A method is provided to crimp a sheet having, before crimping, a thickness, a moisture, a composition, and a width, the method including: obtaining one pre-crimping sheet characteristic among the thickness of the sheet, the moisture of the sheet, the composition of the sheet, and the width of the sheet; crimping the sheet to form a plurality of corrugations on the sheet, the crimping including providing a pair of crimping rollers defining a nip therebetween, the nip having a nip size, and inserting the sheet in the nip; evaluating a post-crimping characteristic of the sheet after crimping; and varying the nip size on the basis of the obtained one of the pre-crimping sheet characteristic, and on the basis of the evaluated post-crimping sheet characteristic. An apparatus to crimp a sheet is also provided.

A method is provided to crimp a sheet having, before crimping, a thickness, a moisture, a composition, and a width, the method including: obtaining one pre-crimping sheet characteristic among the thickness of the sheet, the moisture of the sheet, the composition of the sheet, and the width of the sheet; crimping the sheet to form a plurality of corrugations on the sheet, the crimping including providing a pair of crimping rollers defining a nip therebetween, the nip having a nip size, and inserting the sheet in the nip; evaluating a post-crimping characteristic of the sheet after crimping; and varying the nip size on the basis of the obtained one of the pre-crimping sheet characteristic, and on the basis of the evaluated post-crimping sheet characteristic. An apparatus to crimp a sheet is also provided.

A consumable for use with a non-combustible aerosol provision device including a first aerosol-generating material and a second aerosol-generating material, wherein each of the first and second aerosol-generating materials includes an amorphous solid, the amorphous solid having: 0.5-60 wt % of a gelling agent; 0-80 wt % of an aerosol-former material; and 0-60 wt % of at least one of an active substance, a flavorant and an acid; wherein these weights are calculated on a dry weight basis and wherein the amorphous solid of the first aerosol-generating material has a composition different from a composition of the amorphous solid of the second aerosol-generating material.

A consumable for use with a non-combustible aerosol provision system, the consumable having a sheet of aerosol-generating material including an amorphous solid, wherein the sheet of aerosol-generating material is crimped and gathered; the consumable having a wrapper that circumscribes the aerosol-generating material.

The invention relates to a multilayer reconstituted plant sheet suitable for devices which heat tobacco without burning it, said reconstituted plant sheet having a high density.