The invention relates to a multilayer reconstituted plant sheet suitable for devices which heat tobacco without burning it, said reconstituted plant sheet having a high density.

The subject of the invention is a reconstituted tobacco specifically suitable for devices that heat tobacco without burning it.

The subject of the invention is a reconstituted tobacco specifically suitable for devices that heat tobacco without burning it.

A method for preparing a tar extract with aroma characteristics from a discarded cigarette butt includes: (1) adding the discarded cigarette butt to an extraction solvent, and carrying out subcritical fluid extraction to obtain an extraction solution; (2) carrying out vacuum filtration on the extraction solution, and carrying out vacuum distillation on the filtrate to obtain a concentrated extractum; and (3) carrying out molecular distillation on the concentrated extractum, and collecting a light fraction of the molecular distillation to obtain the tar extract with aroma characteristics. Through the subcritical fluid extraction and separation by the molecular distillation to prepare the tar extract, harmful substances in the tar are removed, and the aroma components are retained. The tar extract is applied to a cigarette to improve the aroma, smoke and taste characteristics of the cigarette.

Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that positively regulates alkaloid biosynthesis, such as nicotine biosynthesis. In particular, the present disclosure provides methods for the inhibition of Nicotiana benthamiana auxin response factor 1 (NbTF1) to reduce alkaloid biosynthesis in plants.

Plant metabolism and alkaloid levels can be regulated by transcription factors that regulate the nicotinic alkaloid biosynthetic pathway. In one embodiment, the disclosure provides a transcription factor that positively regulates alkaloid biosynthesis, such as nicotine biosynthesis. In particular, the present disclosure provides methods for the inhibition of Nicotiana benthamiana auxin response factor 1 (NbTF1) to reduce alkaloid biosynthesis in plants.

The invention relates to a method to unwind a sheet of material wound in a bobbin, the method comprising: providing a bobbin of a coiled sheet, the bobbin defining an outer surface and comprising a free portion of the sheet unwound from the bobbin; arranging a compressing element on the outer surface of the bobbin, so as to define a contact line, the contact line being the line separating the free portion of the sheet from the rest of the bobbin; pressing by means of the compressing element the outer surface of the coiled sheet at the contact line with a force comprised between about (4) Newton and about (16) Newton; and unwinding the sheet from the bobbin pulling the sheet in the unwinding direction.

The invention relates to a method to unwind a sheet of material wound in a bobbin, the method comprising: providing a bobbin of a coiled sheet, the bobbin defining an outer surface and comprising a free portion of the sheet unwound from the bobbin; arranging a compressing element on the outer surface of the bobbin, so as to define a contact line, the contact line being the line separating the free portion of the sheet from the rest of the bobbin; pressing by means of the compressing element the outer surface of the coiled sheet at the contact line with a force comprised between about (4) Newton and about (16) Newton; and unwinding the sheet from the bobbin pulling the sheet in the unwinding direction.

An aerosol generating article includes an aerosol generator including a first aerosol generating material which does not include nicotine; a tobacco filler arranged adjacent to an end of the aerosol generator and including a second aerosol generating material including nicotine; a cooler arranged adjacent to an end of the tobacco filler and configured to cool aerosol; and a mouth piece arranged adjacent to an end of the cooler.

The present invention relates to a method for unwinding a bobbin (12) of a coiled sheet (13), the method comprising the steps of: providing a bobbin of a coiled sheet, the bobbin having a first rotation axis (17) and comprising a free portion (14) of the sheet unwound from the bobbin, the free portion (14) defining a contact line (16) as a line separating the free portion and the remaining of the coiled sheet (13); providing a roller (30) having a second rotation axis substantially parallel to the first rotation axis (17); putting the roller (30) in contact with the bobbin (12) at the contact line (16); unwinding the sheet from the bobbin (12) pulling the sheet in an unwinding direction such that an angle between the free portion of the sheet and a radius of the bobbin at the contact line is comprised between about 110 degrees and about 150 degrees or between about 200 degrees and about 300 degrees; and while unwinding the sheet from the bobbin keeping the roller in contact with the outer surface of the bobbin. The present invention also relates to a kit to unwind a sheet of material wound in a bobbin.