Methods of preparing polymer-filled chromatography resin and their uses are provided.

Provided is a separation method for amine, the separation method including performing liquid chromatography, wherein a separating agent in which a ligand having a crown ether-like cyclic structure is supported on a carrier is used as a stationary phase, and wherein a mobile phase contains an aqueous solution of at least one salt of a hydrophobic anion selected from the group consisting of a salt of a chaotropic anion and a salt of a hydrophobic organic acid.

Magnetic nanoparticle coated porous materials for recovering a contaminant from contaminated water are provided. In embodiments, such a material comprises a porous substrate having a solid matrix defining a plurality of pores distributed through the solid matrix and further comprising a coating comprising magnetic nanoparticles on surfaces of the solid matrix.

A coated substrate (2a, 2b) adapted for hydrocarbon adsorption having at least one surface, and a coating on the at least one surface, the coating comprising particulate carbon and a binder, wherein the particulate carbon has a BET surface area of at least about 1300 m.sup.2/g; and at least one of: (i) a butane affinity of greater than 60% at 5% butane; (ii) a butane affinity of greater than 35% at 0.5% butane; (iii) a micropore volume greater than about 0.2 ml/g and a mesopore volume greater than about 0.5 ml/g. A bleed emission scrubber (1) and an evaporative emission control canister system (30) comprising the coated substrate (2a, 2b) are provided. They can control evaporative hydrocarbon emissions and may provide low diurnal breathing loss (DBL) emissions even under a low purge condition.

An antimicrobial filter media, which includes a non-woven fabric; and an antimicrobial agent bound to the nonwoven fabric by a binder, the antimicrobial agent including silver sodium zirconium hydrogenphosphate and thiabendazole, and the silver sodium zirconium hydrogenphosphate and the thiabendazole are employed at a weight ratio of 1:1.5 to 1.5:1, to an air cleaner including the same, and to a process for preparing the same. The antimicrobial filter media includes silver sodium zirconium hydrogenphosphate and thiabendazole, as an antimicrobial agent, at a specific weight ratio. As a result, it is possible to effectively filter harmful microorganisms to supply purified air, to have excellent antibacterial, antiviral, and antifungal properties at the same time, and to further enhance the durability and lifespan characteristics by virtue of excellent filter damage prevention effect.

In various embodiments, the present disclosure pertains to organic polymer core-shell particles that comprise a non-porous organic polymer core (i.e., having a pore volume of less than 0.1 cc/g) and a porous organic polymer shell (i.e., having a pore volume of greater than 0.1 cc/g), in which the porous organic polymer shell has a pore size ranging from 100 Å to 3000 Å. In some embodiments, the present disclosure pertains to chromatographic separation devices that comprise such organic polymer core-shell particles. In some embodiments, the present disclosure pertains to chromatographic methods that comprise: (a) loading a sample onto a chromatographic column comprising such organic polymer core-shell particles and (b) flowing a mobile phase through the column.

The invention relates to poly-amide bonded hydrophilic interaction chromatography (HILIC) stationary phases and novel HILIC methods for use in the characterization of large biological molecules modified with polar groups, known to those skilled in the art as glycans. The invention particularly provides novel, poly-amide bonded materials designed for efficient separation of large biomolecules, e.g. materials having a large percentage of larger pores (i.e. wide pores). Furthermore, the invention advantageously provides novel HILIC methods that can be used in combination with the stationary phase materials described herein to effectively separate protein and peptide glycoforms by eliminating previously unsolved problems, such as on-column aggregation of protein samples, low sensitivity of chromatographic detection of the glycan moieties, and low resolution of peaks due to restricted pore diffusion and long intra/inter-particle diffusion distances.

The present disclosure discloses a cellulose nanocrystal-supported sodium alginate adsorbent and use thereof in enriching organic phosphorus in wastewater, belonging to the technical field of environmental engineering. Cellulose nanocrystals are prepared from papermaking deinking sludge through chemical conditioning, drying, crushing, chemical oxidation and microwave assisted separation, and then supported on alginate under weakly acidic conditions to provide the cellulose nanocrystals with the ability to enrich organic phosphorus in wastewater. By using this method, the content of organic phosphorus in the wastewater can be significantly reduced, the total phosphorus in the effluent can be reduced, and the wastewater treatment effluent can satisfy the discharge standard. The enriched organic phosphorus can be recycled as a phosphorus resource through incineration. In addition, the papermaking deinking sludge is made into the product with a high added value, so the applicability of the papermaking deinking sludge is broadened. The present disclosure has high feasibility.

The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid,
wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.

The present invention is to provide a novel adsorbent which is low in cost, has versatility and high adsorption ability. Specifically, the present invention is to provide an adsorbent of a specific metal element containing a metal salt of a cyanometallic acid, a method for producing the same, and a method for removing the ion of the element that is the target of adsorption using such an adsorbent.