The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.

Disclosed is a separation material comprising hydrophobic polymer particles and a coating layer covering at least a portion of a surface of the hydrophobic polymer particles, wherein the coating layer comprises a hydrophilic polymer having hydroxy groups, and the hydrophilic polymer has a group represented by NHR-L or an epoxy group, wherein R represents a hydrocarbon group and L represents a carboxy group or an amino group.

A method for modifying a support material, in particular a polymeric support material, for use as a stationary phase in an analytical or preparative separation process. The method includes the steps of providing a support material, coating the support material with an oligoamine or polyamine and reacting the support material with a compound comprising a first functional group reactive with amines and/or hydroxy group and an ion-exchange group. Additionally a support material, a chromatography column, a method of chromatographic separation of analytes and the use of such support material.

A chromatographic media for separating bio-polymers, the chromatographic media having cationic exchange properties and anionic exchange properties, the chromatographic media comprising: (a) non-porous substrate particles including an organic polymer, the substrate particles having a neutral hydrophilic layer at a surface of the non-porous substrate particles, in which the neutral hydrophilic layer is configured to reduce a binding of the bio-polymers directly to the non-porous substrate particles compared to a binding of the bio-polymer to the non-porous substrate particles without the neutral hydrophilic layer; (b) a charged first ion exchange layer bound to the substrate particles on top of the hydrophilic layer, the first ion exchange layer comprising first ion exchange groups; and (c) a charged second ion exchange layer bound to the substrate particles on top of the first ion exchange layer.

The present disclosure relates to a method for the isolation of microvesicles comprising contacting the sample with at least one polysaccharide to isolate the microvesicles.

The present invention provides a functionalised polymeric chromatography medium, prepared by a process which comprises (i) providing a substrate formed of one or more polymer nanofibres, (ii) grafting one or more neutral polymer chains from the substrate, and (iii) contacting the grafted product with a reagent which functionalises the product of step (ii) as a chromatography medium, wherein step (ii) comprises reacting a plurality of compounds of formula and/or its enantiomers, and/or its derivatives of formula (I) and/or enantiomers and/or diastereomers thereof: with one or more functional groups present on the nanofibre substrate, wherein R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 may be the same or different, and are chosen from H, halogen, C.sub.1-C.sub.4 alkyl, or C.sub.1-C.sub.4 alkoxy provided that at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4 or R.sub.5 is not hydrogen.

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The invention provides methods for preparing a polymer-grafted and functionalized nonwoven membrane adapted for use in separation processes. The invention further provides so-formed membranes as well as improved separation methods utilizing the membranes. The polymer-grafted and functionalized nonwoven membranes are particularly formed utilizing thermal grafting. In particular, an acrylate or methacrylate polymer can be grafted onto a nonwoven web comprising a plurality of polymeric fibers to form a plurality of polymer segments covalently attached to the polymeric fibers. Thermal grafting particularly can comprise using a thermal initiator and exposing the nonwoven web to heat to initiate polymerization of the acrylate or methacrylate monomer. The grafted polymeric fibers can be functionalized to attach at least one functional group adapted for binding to a target molecule to the polymer segments of the grafted polymeric fibers.

An apparatus for collecting mineral particles in a slurry or the tailings is disclosed. The apparatus may take the form of a filter, a conveyor belt or an impeller to be used in a processor to collect mineral particles in the slurry, or in a tailings pond to collect mineral particles in the tailings. The filter, conveyor belt or impeller has a collection area made of or coated with a polymer or a polymer-coated material having a functional group, either anionic or cationic to attach to the mineral particles. Alternatively, the synthetic material has hydrophobic molecules to render the collection area hydrophobic. When the mineral particles in the slurry or tailings are combined with collector molecules, the mineral particles also become hydrophobic. The hydrophobic mineral particles are attracted to the hydrophobic collection area. The filter, conveyor belt and impeller may have a plurality of passage ways in order to increase the contacting surfaces.

The present invention relates to an adsorption medium including polymeric space elements which have been bonded to the surface of the chromatography matrix, and polymer chains containing chromatographically active centers, wherein the polymer chains have been bonded to the polymeric spacer elements, as well as to a method for the production thereof, and to the use of the adsorption medium for the purification of biomolecules.

Chromatography devices and methods for forming and using the devices are described. The devices include a polyimide-based support phase and a polymer grafted to a surface of the polyimide-based support phase. A microwave-assisted graft polymerization protocol is described to form the polymer at the surface of the support phase. Devices can be utilized in high-efficiency separation of macromolecules such as proteins.