Compositions and methods are provided for use in controlling souring and corrosion causing prokaryotes, such as SRP, by treating oil and gas field environments or treatment fluids with a newly identified bacterial strain ATCC Accession No. PTA-124262 as a self-propagating whole cell that produces an anti-SRP bacteriocin in situ. In another aspect, the methods use one or more toxic peptides or proteins isolated therefrom in methods to control unwanted prokaryotic growth in these environments.

The present invention discloses a Pseudomonas stutzeri strain, named Pseudomonas stutzeri EBT-2, which was deposited in China Center for Type Culture Collection under Deposit No. CCTCC M 2019731 on Sep. 17, 2019. The present invention also discloses a composite microbial inoculum which is prepared by mixing an expanded culture solution of a Pseudomonas balearica EBT-1 with Deposit No. CCTCC M 2019730 and an expanded culture solution of the Pseudomonas stutzeri EBT-2 with Deposit No. CCTCC M 2019731 in a volume ratio of 1:1. The present invention finally discloses use of the composite microbial inoculum in treating membrane concentrate of landfill leachate. The composite microbial inoculum is capable of implementing high-efficiency biological denitrification of the membrane concentrate of landfill leachate.

A method for improved anaerobic digestion is presented. The method includes mixing a volume of waste material with water to form a feedstock mixture. The volume of waste material includes an initial amount of biomass and the feedstock mixture includes methanogenic bacteria either naturally present in the waste material or introduced artificially. The method also includes introducing one or more promoter substances to the feedstock mixture. The one or more promoter substances are capable of modifying the methanogenic bacteria. Modifying includes stimulating novel enzyme production in the methanogenic bacteria.

A permeable reactive barrier having two or more layers of a geotextile fabric inoculated with a bioremediation microbe is provided. The permeable reactive barrier further includes two or more layers of coarse-grained geological material separating the two or more layers of geotextile fabric such that any pair of adjacent layers of geotextile fabric is separated by a layer of coarse-grained geological material. The permeable reactive barrier includes a perforated metal casing surrounding and containing the layers of coarse-grained geological materials and geotextile fabric.

A permeable reactive barrier having two or more layers of a geotextile fabric inoculated with a bioremediation microbe is provided. The permeable reactive barrier further includes two or more layers of coarse-grained geological material separating the two or more layers of geotextile fabric such that any pair of adjacent layers of geotextile fabric is separated by a layer of coarse-grained geological material. The permeable reactive barrier includes a perforated metal casing surrounding and containing the layers of coarse-grained geological materials and geotextile fabric.

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination/incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use/consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

The present disclosure relates to processes for treating wastewater such as acid rock drainage. The processes may, for example, comprise subjecting the wastewater to a microbial fuel cell process, neutralizing the acid with a base comprising calcium to produce an aqueous composition comprising calcium ions and subjecting the aqueous composition comprising calcium ions to a biological precipitation process to precipitate the calcium ions as calcium carbonate.

The present invention relates to a method for treating wastewater containing a furan compound, a microbial preparation for use in the method, a method for high density culture of a microorganism for use in the above method, and a method for producing a microbial preparation by using the culture method.

The present invention relates to a method for treating wastewater containing a furan compound, a microbial preparation for use in the method, a method for high density culture of a microorganism for use in the above method, and a method for producing a microbial preparation by using the culture method.