A methodology, system and apparatus are provided that include anoxic biofilms to perform partial denitrification and anammox (PdNA) reactions. The PdNA reactions can facilitate process intensification and carbon efficient biological nitrogen removal. The anoxic biofilms can be placed in a pre-anoxic zone or a downstream anoxic zone, where the biofilm and reactions are managed, including using storage compounds, to overcome mass transfer limitations in the biofilm. The methodology, system and apparatus can, when compared to state-of-the art technologies, improve the concentration gradient or reduce mass transfer limitations to facilitate PdNA reactions.

A process for constructing a soil-based rhizosphere flow-through system to break down contaminants in contaminated water. The process includes the steps of: providing plants planted in soil in a test bioreactor, the plants providing a rhizosphere; exposing the rhizosphere to the contaminated water; extracting microorganisms from the rhizosphere following their exposure to the contaminated water; preparing a microbial suspension from the extract; subjecting the microbial suspension to growth conditions to increase the concentration of the microorganisms, thereby preparing a soil conditioner; adding the soil conditioner to soil in a contained area having a water flow inlet and outlet; and planting a plurality of plants in the soil, the plants being of the same species as the plants of the test bioreactor.

A wastewater treatment system including a contact tank, a dissolved air flotation unit, a fermentation unit, and a biological treatment unit is disclosed. A method of retrofitting a wastewater treatment system by arranging the wastewater treatment system such that floated biosolids are fermented in an anerobic environment and fluidly connecting the biological treatment unit to receive at least a portion of the fermented solids is also disclosed. The method optionally includes providing a fermentation unit and fluidly connecting the fermentation unit to a biological treatment unit. A method of treating wastewater including combining the wastewater with activated sludge, floating biosolids from the activated wastewater, fermenting the floated biosolids, and biologically treating the effluent with the fermented solids is also disclosed. A method of facilitating delivery of soluble organic carbon to a biological treatment unit is also disclosed.

A method is described for recovering resources from a microbe supporting environment such as a water treatment system, comprising the steps of using microbial DNA sequencing to analyze the microbiome of the microbe supporting environment and identifying adjustments to the microbial content of the microbiome that will be useful in extracting resources from the microbe supporting environment such as a water treatment system, wherein the resources extracted can include, for example, methane released by microbes, nitrogen, phosphorus, or other contaminants generated by microbes, and/or clean water obtained by removing contaminants in a water treatment system.

The present invention relates to a process and device for selectively removal of deflocculated sludge below a pre-selected size from a sludge mixed liquor. The invention resides in the concept of removing from the sludge mixed liquor deflocculated sludge. The removal is preferably carried out by a two-step separation providing a fraction comprising sludge flocs, substantially freed from deflocculated sludge, and a fraction containing mostly deflocculated sludge. The fraction comprising sludge flocs is returned into the sludge mixed liquor, while the fraction containing mostly deflocculated sludge is removed from the system.

Exemplary embodiments describe apparatuses and related processes for improving mixing and sheer in a fixed film reactor. One process can include recycling at least a portion of a biogas product through at least one sparger below a fixed film zone in the fixed film reactor at conditions sufficient for mixing and sheering the film from an internal structure within the fixed film zone. Often, a cross-sectional area of the fixed film zone fills at least about 90% of a cross-sectional area of the fixed film reactor.

Provided is a process comprising receiving overflow of wastewater influent from a clarifier basin in a clarifier effluent collection trough; receiving inflow of wastewater influent from the clarifier effluent collection trough in a filter influent flow inlet distribution channel; maintaining substantially constant liquid level in the filter influent inlet distribution channel; applying hydrostatic pressure to push wastewater influent from the filter influent flow inlet distribution channel into an upflow backwash filter contusing denitrifying biomass or deammonification biomass; backwashing the backwash filter with a gas lift backwash flow; returning filter reject backwash wastewater from rejection compartment of the filter through denitrifying bacteria or deammonification biomass recycle return line to a location upstream of the filter; and recycling denitrifying bacteria or deammonification biomass from denitrifying bacteria or deammonification biomass recycle return line to at least one of the clarifier effluent collection trough, filter influent flocculation tank, or filter influent flow distribution channel.

A method of exposing microorganisms to a condition wherein the condition facilitates or inhibits growth of a specific species of microorganisms or a specific consortium of microorganisms. The condition may also stimulate microorganisms to uptake a pollutant from a fluid and/or release the pollutant. Furthermore, the microorganisms may also be harvested and used as a foodstuff for human and animal consumption.

The present invention provides a method of producing a product for waste degradation, a product for waste degradation, an isolated microbial strain, a substantially pure culture of the isolated microbial strain, a microbial consortium or a mixed microbial composition, and a waste degradation method. The method of producing a product for waste degradation includes: isolating a plurality of microbial strains from one or more sources of food waste as separate colonies on a solid medium; selecting a plurality of the isolated microbial strains based on size and/or abundance of colonies of each of the microbial strains, the selected microbial strains being selected based on having a larger colony size and/or a higher colony abundance compared to other microbial strains; and combining the selected microbial strains to produce a microbial consortium for waste degradation.

The present invention relates to the field of biotechnology, and discloses a composition capable of promoting the growth of denitrifying microorganisms and uses thereof. The composition contains a glycolipid, an alditol, and a salt of organic acid, wherein the glycolipid is at least one of trehalose glycolipid, sophorolipid and rhamnolipid. The composition provided in the present invention can promote the growth of denitrifying microorganisms, and thereby improve the denitrification activity of the denitrifying microorganisms, promote a smooth denitrification process, and shorten the processing time.