The present invention discloses an equal energy deformation composite foundation using microorganism to solidify aggregate and a construction method thereof, the composite foundation comprises a pile body and a cushion layer, wherein the pile body is provided with several piles, the cushion layer is arranged at the top of the pile body, the pile body is connected into an integral structure through the cushion layer, and the pile body and the cushion layer are filled with aggregate solidified by microorganism. The method comprises the following steps: step 1, leveling the site; Step 2, construction preparation; Step 3, the pile driver in place; Step 4, forming a hole by hammering; Step 5, filling aggregate into the hole; Step 6, repeating the work of step 5; Step 7, forming an equal energy deformation compaction pile using microorganism to solidify aggregate; Step 8, moving to the next pile; Step 9, tamping the ground; Step 10, until the cushion is flush with the surface. Beneficial effects: using local materials, turning waste into wealth, being environmental friendly, saving project cost and conforming to the concept of green development.

The present invention discloses an equal energy deformation composite foundation using microorganism to solidify aggregate and a construction method thereof, the composite foundation comprises a pile body and a cushion layer, wherein the pile body is provided with several piles, the cushion layer is arranged at the top of the pile body, the pile body is connected into an integral structure through the cushion layer, and the pile body and the cushion layer are filled with aggregate solidified by microorganism. The method comprises the following steps: step 1, leveling the site; Step 2, construction preparation; Step 3, the pile driver in place; Step 4, forming a hole by hammering; Step 5, filling aggregate into the hole; Step 6, repeating the work of step 5; Step 7, forming an equal energy deformation compaction pile using microorganism to solidify aggregate; Step 8, moving to the next pile; Step 9, tamping the ground; Step 10, until the cushion is flush with the surface. Beneficial effects: using local materials, turning waste into wealth, being environmental friendly, saving project cost and conforming to the concept of green development.

The invention is directed to compositions and methods for the manufacture of pigmented solids structures for which can be used for construction and/or decoration. Manufacturing comprises fixing one or more pigments to an aggregate material such as crushed rock, stone or sand. The pigmented aggregate is incubated with urease or urease producing microorganisms, an amount of a nitrogen source such as urea, and an amount of calcium source such as calcium chloride forming calcite bridges between particles of aggregate. The resulting solid has a hardness and colorfastness for most any construction material. Using selected aggregate and pigment, the process also provides for the manufacture of simulated-stone materials such as clay or granite bricks or blocks, marble counter-tops, and more. The invention is also directed to composition containing microorganisms and pigment as kits that can be added to most any aggregate materials.

The invention is directed to compositions and methods for the manufacture of pigmented solids structures for which can be used for construction and/or decoration. Manufacturing comprises fixing one or more pigments to an aggregate material such as crushed rock, stone or sand. The pigmented aggregate is incubated with urease or urease producing microorganisms, an amount of a nitrogen source such as urea, and an amount of calcium source such as calcium chloride forming calcite bridges between particles of aggregate. The resulting solid has a hardness and colorfastness for most any construction material. Using selected aggregate and pigment, the process also provides for the manufacture of simulated-stone materials such as clay or granite bricks or blocks, marble counter-tops, and more. The invention is also directed to composition containing microorganisms and pigment as kits that can be added to most any aggregate materials.

The invention is directed to kits, compositions, tools and methods comprising a cyclic industrial process to form biocement. In particular, the invention is directed to materials and methods for decomposing calcium carbonate into calcium oxide and carbon dioxide at an elevated temperature, reacting calcium oxide with ammonium chloride to form calcium chloride, water, and ammonia gas; and reacting ammonia gas and carbon dioxide at high pressure to form urea and water, which are then utilized to form biocement. This cyclic process can be achieved by combining industrial processes with the resulting product as biocement. The process may involve retention of calcium carbonate currently utilized in the manufacture of Portland Cement.

The invention is directed to kits, compositions, tools and methods comprising a cyclic industrial process to form biocement. In particular, the invention is directed to materials and methods for decomposing calcium carbonate into calcium oxide and carbon dioxide at an elevated temperature, reacting calcium oxide with ammonium chloride to form calcium chloride, water, and ammonia gas; and reacting ammonia gas and carbon dioxide at high pressure to form urea and water, which are then utilized to form biocement. This cyclic process can be achieved by combining industrial processes with the resulting product as biocement. The process may involve retention of calcium carbonate currently utilized in the manufacture of Portland Cement.

A method for producing a carbonate-containing species-rich, nitrogen-containing species-free solution from a urea-rich solution is proposed. The method comprising the steps of: providing a first reservoir comprising a first mixture including urea and a catalyser comprising an enzymatic catalyser and/or a microorganism; allowing an enzymatic reaction catalysed by the catalyser to decompose urea, thereby obtaining a second mixture comprising nitrogen-containing species and carbonate-containing species; converting at least some of the nitrogen-containing species into gaseous nitrogen-containing species to obtain a third mixture comprising the gaseous nitrogen-containing species and the carbonate-containing species; filtering the third mixture by a gas- permeable filter, thereby separating at least some of the gaseous nitrogen-containing species from the carbonate-containing species while keeping the catalyser away from the gas-permeable filter; and collecting the so-obtained carbonate-containing species-rich, nitrogen-containing species-free solution.

A method for producing a carbonate-containing species-rich, nitrogen-containing species-free solution from a urea-rich solution is proposed. The method comprising the steps of: providing a first reservoir comprising a first mixture including urea and a catalyser comprising an enzymatic catalyser and/or a microorganism; allowing an enzymatic reaction catalysed by the catalyser to decompose urea, thereby obtaining a second mixture comprising nitrogen-containing species and carbonate-containing species; converting at least some of the nitrogen-containing species into gaseous nitrogen-containing species to obtain a third mixture comprising the gaseous nitrogen-containing species and the carbonate-containing species; filtering the third mixture by a gas- permeable filter, thereby separating at least some of the gaseous nitrogen-containing species from the carbonate-containing species while keeping the catalyser away from the gas-permeable filter; and collecting the so-obtained carbonate-containing species-rich, nitrogen-containing species-free solution.

Methods for repair of cracks and niches in concrete using carbonic anhydrase embedded in a gelatin, hydrogel, or other aqueous matrix are provided. A durable corrosion resistant concrete mix containing carbonic anhydrase is provided, as are methods of making and using the durable corrosion resistant concrete. Corrosion resistance and durability derive from decreased porosity compared to concrete without the enzyme, conferring decreased access of deleterious materials such as chloride ions to interior portions of the concrete.

Methods for repair of cracks and niches in concrete using carbonic anhydrase embedded in a gelatin, hydrogel, or other aqueous matrix are provided. A durable corrosion resistant concrete mix containing carbonic anhydrase is provided, as are methods of making and using the durable corrosion resistant concrete. Corrosion resistance and durability derive from decreased porosity compared to concrete without the enzyme, conferring decreased access of deleterious materials such as chloride ions to interior portions of the concrete.