The present teachings relate to methods, systems, and kits for the preparation, purification and/or analysis of a glycan or glycoconjugate, and specifically to a magnetic bead based sample preparation protocol. In some aspects, the sample preparation protocol can provide for glycoconjugate capture, glycan release, fluorescent derivatization, and glycan purification for subsequent capillary electrophoresis, liquid chromatography, or other glycoanalytical method using magnetic beads containing negatively charged carboxyl groups extending from the surface of the magnetic beads.

A Dendrobium officinale oligosaccharide, a Dendrobium officinale oligosaccharide derivative and a preparation method and use thereof. The Dendrobium officinale oligosaccharide includes 3-9 glycoside residues and a glucose residue at a non-reducing end; the derivative thereof is obtained by substituting with a hydrophobic residue at the non-reducing end; the preparation method and use of both are also provided.

Disclosed is a process for obtaining glycan extracts from apple pomace in which a mixture comprising apple pomace and water is extracted into water so that an aqueous glycan extract is obtained; at least one alcohol is added to the extract, whereby a solid glycan extract is precipitated; and the solid glycan extract is separated from the liquid. Also disclosed is a process for producing functionalized glycan wherein the glycan is converted using at least one amine into at least one functionalized glycan. Also disclosed are a functionalized glycan that is produced by the method and uses thereof

Disclosed is a method for extracting useful substances from shrimp shells. The method comprises: crushing the shrimp shells, mixing the crushed shrimp shells and water, then heating same to 28° C.-35° C., adjusting the pH value to 6.8-7.5, preferably 6.8-7, then adding an alkaline protease and mixing same, heating same to 42° C.-48° C., performing constant-temperature enzymolysis for 50-70 min, and performing sieving to obtain an enzymatic hydrolysate and solid residues; performing centrifugal separation treatment on the enzymatic hydrolysate to obtain a shrimp protein deposit containing astaxanthin; mixing the shrimp protein deposit and water, performing heating while stirring, adjusting the pH value to 6.8-7.0, performing heating to 58° C.-60° C., adding vegetable oil, and performing emulsification for 50-70 min under stirring to obtain an emulsion; and performing centrifugation on the emulsion, and performing delamination to obtain astaxanthin-containing oil in an upper layer, water in a middle layer, and a shrimp protein in a lower layer. The method of the present invention uses waste biomass obtained after shrimps processed as a raw material, and can simultaneously extract several high-value substances, thereby not only improving the utilization rate of the raw material, but also shortening the production cycle; and no organic solvent is added, such that the method is clean, green and environmentally friendly.

The invention relates to a low molecular weight sulfate chondroitin and a preparation method thereof. A low molecular weight chondroitin sulfate with the average molecular weight of less than 1000 Dalton can be obtained by a production process of chondroitin sulfate lyase degradation, deproteinization, filtration and sterilization and drying using macromolecular sulfate chondroitin as a raw material. The low molecular weight Chondroitin sulfate has a narrow molecular weight distribution range, the ratio of chondroitin sulfate disaccharide is 43˜60% and the ratio of chondroitin sulfate tetrasaccharide is 30˜45%, the sum of chondroitin sulfate disaccharide and chondroitin sulfate tetrasaccharide is more than 87%, the total oligosaccharide content of low molecular weight chondroitin sulfate is more than 97% and the protein content is less than 0.5%; Compared with the general market macromolecule chondroitin sulfate, the product has more remarkable repair effect at the concentration of 50˜100 μg/mL on chondrocytes damaged by 1 mM hydrogen peroxide, with strong repair ability and repair rate of 14%˜23%. The low molecular weight chondroitin sulfate can be used to treat joint injury and is an important raw material for medical products, health care products, cosmetics and food.

This invention relates to a method for the enzymatic synthesis of oligosaccharides, preferably human milk oligosaccharides (HMOs) The method comprises the enzymatic transfer of a glycosyl moiety and subsequent removal of by-products, such as lactose, by nanofiltration using a membrane comprising an active polyamide layer.

Provided are methods for preparing modified natural fiber composite feedstocks. In some embodiments, the presently disclosed methods include hydrolyzing agricultural fiber material, optionally soybean hulls, under conditions and for a time sufficient to remove some or all of the arabinose from the agricultural fiber material to produce an arabinose-deficient hydrolyzed product; hydrolyzing the arabinose-deficient hydrolyzed product under conditions and for a time sufficient to remove some or all of the xylose from the arabinose-deficient hydrolyzed product to produce a hydrolyzed fiber material; and combining a thermoplastic copolyester (TPC) with up to 35 wt. % by weight of the hydrolyzed material, whereby a modified fiber composite feed stock is prepared. Also provided are methods for isolating xylose removed from arabinose-deficient hydrolysates, modified fiber composites prepared by the presently disclosed methods, method for 3D printing structure using the modified fiber composites, methods for improving at least one characteristic of modified TPC composites, and methods for improving fused filament fabrication (FEE) processes.

The present disclosure provides a heteropoly oligosaccharide and an application thereof in improving plant disease resistance. The heteropoly oligosaccharide includes seven D-glucose residues and one D-galactose residue. Each heteropoly oligosaccharide molecule comprises R.sub.1 and R.sub.2, R.sub.1 being H or a monomolecular pyruvate group, R.sub.2 being H or a monomolecular succinyl group, and the structure thereof is represented as follows:

A preparation of a homogeneous polysaccharide from a Radix Puerariae aqueous extract is provided and the lipid-lowering activity of the polysaccharide is studied. It is proved through in vivo experiments that the homogeneous polysaccharide QL extracted from Radix Puerariae (a genuine medicinal material in Jiangxi Province) in the present disclosure has significant lipid-lowering activity, can significantly reduce a serum triglyceride content and a liver index, and can be used to develop a potential safe and effective lipid-lowering drug.

Fluid contaminants may be prevalent in many industries, such as the mining industry. Functionalized saccharide polymers comprising two or more monosaccharide units linked by glycosidic bonds and having a portion of the monosaccharide units oxidatively opened and functionalized with at least one aminocarboxylic acid covalently bound through nitrogen at a site of oxidative opening may be utilized in conjunction with fluid remediation processes, such as froth flotation. In non-limiting examples, the functionalized saccharide polymers may also be useful for promoting dust control, particulate coating, clay stabilization, and various subterranean treatment operations. Glycine represents one example of an aminocarboxylic acid that may be covalently bonded through nitrogen at a site of oxidative opening.