Among the various aspects of the present disclosure is the provision of compositions and methods for platelet storage supplementation with an amino acid, such as taurine.

The invention relates to methods for the isolation of non-haematopoietic tissue-resident lymphocytes, particularly γδ T cells. Such γδ T cells include non-Vδ2 cells, e.g. Vδ1, Vδ3 and Vδ5 cells and such non-haematopoietic tissues include skin and gut. It will be appreciated that such isolated non-haematopoietic tissue-resident lymphocytes find great utility in adoptive T cell therapies, chimeric receptor therapies and the like. Also provided are methods for expanding isolated tissue-resident lymphocytes, particularly methods for isolating and expanding γδ T cells. The present invention also relates to both individual cells and populations of cells produced by the methods described herein.

The invention relates to a device for cryogenic freezing of units for packaging a biological substance comprising a receptacle (2) configured such that a stream of vapour of a cryogenic agent circulates therein, having at its top an insertion opening (10) for inserting the packaging units into the receptacle (2), a lid (3) that is able to move between a closed position in which it closes the insertion opening (10) and an open position in which it leaves the insertion opening (10) open, and a port (4) for discharging the vapour, provided in the receptacle (2), characterised in that the device (1) further comprises a stopper (13) mechanically connected to the lid (3) such that, when the lid (3) is in the closed position, the stopper (13) is spaced apart from the port (4) and, when the lid (3) is in the open position, the stopper (13) closes the port (4).

An apparatus for perfusing an organ includes: an organ chamber configured to provide physical protection to an organ; at least one fluid flow loop from an outlet connection of the organ chamber, through an oxygenator, through a pump, and to an inlet connection of the organ chamber; and a container enclosing the components of the apparatus, wherein the apparatus is portable.

A bag holder for supporting a bioprocess bag during freezing includes a cover, a bottom, a frame, and at least one bag support. The cover, the bottom, and the frame form an enclosed space. The at least one bag support is disposed in the space. The cover and the bottom are made of metal. The frame has a first side, a second side, a front end, and a back end. Each of the cover and the bottom has a first bendable tab and a second bendable tab configured to bend over the front end from a longitudinal direction to a thickness direction, and a first arm and a second arm extending in the thickness direction and then in the longitudinal direction. Each of the first side and the second side includes at least one recessed portion extending in the longitudinal direction.

The present disclosure discloses a preparation method and a recovery method of pariduval mesenchymal stem cells (PMSCs). In the preparation method, a high-glucose Dulbecco's Modified Eagle Medium (DMEM) that includes a Tryple-ethylenediaminetetraacetic acid (EDTA) enzyme of 40% to 60% in volume concentration and collagenase type II of 8 mg/ml to 12 mg/ml is used as a tissue digestion solution to digest tissue blocks, which facilitates PMSCs to climb out of the tissue blocks and grow adherently; and a serum-free DMEM is adopted as a selective medium to terminate the digestion and resuspend PMSCs, which helps to improve a purity of PMSCs, accelerate the growth of PMSCs, and achieve the rapid expansion of PMSCs in vitro.

An aqueous stabilizing composition for preserving a bodily fluid at ambient temperature is provided. The aqueous stabilizing composition comprises: a sugar selected from a monosaccharide, a disaccharide, or a combination thereof; a buffering agent; a C.sub.1-C.sub.6 alkanol; boric acid, a salt of boric acid, or a combination thereof; and a chelating agent; wherein the composition has a pH of from 4.5 to 5.2. A method for preserving a bodily fluid using the aqueous stabilizing composition is also provided, the method comprising: a) obtaining a sample of the bodily fluid; b) contacting the bodily fluid with the aqueous stabilizing composition to form a mixture; c) mixing the mixture of (b) to form a homogeneous mixture; and d) storing the homogeneous mixture at ambient temperature.

In one representative embodiment, a method of perfusing organs in a patient's body is provided. The method comprises isolating the visceral arteries and the visceral veins from blood circulating through the patient's heart and perfusing the visceral arteries, the visceral veins, and the abdominal organs with a perfusion fluid that is fluidly separated from the blood circulating through the patient's heart. While the visceral arteries and the visceral veins are isolated, and the visceral arteries, the visceral veins, and the abdominal organs are being perfused, the patient's blood is allowed to continue to circulate through the heart.

The present invention relates to a composition for cryopreservation, comprising: a nucleic acid structure which comprises a scaffold nucleic acid folded at predetermined positions to form multiple strands, and a plurality of staple nucleic acids wherein at least a portion of a sequence thereof comprises a complementary sequence to that of the scaffold nucleic acid, which are bound to at least one of the strands of the scaffold nucleic acid to form a double strand; linkers coupled to at least one of single strands in the nucleic acid structure; and an anti-freezing peptide coupled to at least one of the linkers, so as to exhibit excellent freeze-protection effects, which in turn increase cell viability during cryopreservation of cells and tissues, while retaining original texture of food even when used for freezing the food.

The present invention relates to a composition for cryopreservation, comprising: a nucleic acid structure which comprises a scaffold nucleic acid folded at predetermined positions to form multiple strands, and a plurality of staple nucleic acids wherein at least a portion of a sequence thereof comprises a complementary sequence to that of the scaffold nucleic acid, which are bound to at least one of the strands of the scaffold nucleic acid to form a double strand; linkers coupled to at least one of single strands in the nucleic acid structure; and an anti-freezing peptide coupled to at least one of the linkers, so as to exhibit excellent freeze-protection effects, which in turn increase cell viability during cryopreservation of cells and tissues, while retaining original texture of food even when used for freezing the food.