The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.

The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates.

The present invention relates to compositions for and methods of optically imaging tissues or cells using imaging agents having desirable in vivo properties that result in improved signal-to-background ratio.

Provided herein are heptamethine cyanine dyes having a large Stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large Stokes shift dyes as fluorescence resonance energy transfer (FRET) acceptors or donors.

The present invention aims at providing a novel indocyanine compound solving problems of conventionally used indocyanine green, such as solubility in water or physiological saline, a synthesis method and a purification method thereof, and a diagnostic composition including the novel indocyanine compound. Further, provided are a method for evaluating biokinetics of the novel indocyanine compound and a device for measuring biokinetics, and a method and a device for visualizing circulation of fluid such as blood in a living body, which utilize the diagnostic composition. Also, found are a novel indocyanine compound in which a hydrophobic moiety in a near-infrared fluorescent indocyanine molecule is included in a cavity of a cyclic sugar chain cyclodextrin to cover the hydrophobic moiety in the indocyanine molecule with the glucose, and a synthesis method and a purification method thereof. Furthermore, found are a method for fluorescence-imaging an organ other than liver by intravenous administration, a method for evaluating biokinetics of the novel indocyanine compound, a device for measuring biokinetics, and a method and a device for visualizing circulation of fluid such as blood in a living body, utilizing the diagnostic composition including the novel indocyanine compound.

Compounds used as labels with properties comparable to known fluorescent compounds. The compounds can be conjugated to proteins and nucleic acids for biological imaging and analysis. Synthesis of the compounds, formation and use of the conjugated compounds, and specific non-limiting examples of each are provided.

Nonlinear optical chromophores having one or more diamondoid groups covalently attached to the chromophore, methods of making nonlinear optical chromophores, their use in thin films and electro-optical devices containing such nonlinear optical chromophores and thin films comprising the same.

The present invention relates to an optical filter containing: an absorption layer containing a near-infrared absorbing dye (A) having a maximum absorption wavelength in dichloromethane being in a wavelength range of 850 to 1,100 nm and a transparent resin; and a reflection layer formed of a dielectric multilayer film, in which the near-infrared absorbing dye (A) satisfies the following Formulae (1) to (4).

[00001]$\begin{array}{cc}\mathrm{AB}{S}_{400\left(A\right)\mathrm{DCM}}/\mathrm{AB}{S}_{\max \left(A\right)\mathrm{DCM}}<0.1,& \left(1\right)\end{array}$$\begin{array}{cc}\mathrm{AB}{S}_{550\left(A\right)\mathrm{DCM}}/\mathrm{AB}{S}_{\max \left(A\right)\mathrm{DCM}}<0\text{.04},& \left(2\right)\end{array}$$\begin{array}{cc}\mathrm{AB}{S}_{400\left(A\right)\mathrm{TR}}/\mathrm{AB}{S}_{\max \left(A\right)\mathrm{TR}}<0.15,\mathrm{and}& \left(3\right)\end{array}$

This disclosure provides cyanine dye compounds having linked hydrogen bond-forming groups that can be used for detecting nucleic acids, particularly for fluorescent staining of DNA, in a biological sample. We found that the simultaneous incorporation of at least two hydrogen bond-forming groups (HBGs) into such a nucleic acid stain can provide for crosslinking interactions between the stain and the target nucleic acid, and lead to a significant increase in the detection sensitivity without substantially increasing undesirable cytotoxicity or mutagenicity properties of the dye compounds. The cyanine dye compounds can have a cyanine structure that connects two particular fused heterocycle ring systems, where at least two HBGs are connected to the core structure through linkers. Also provided are nucleic acid complexes including the cyanine dye compounds, and methods of using the compounds to detect nucleic acids in a biological sample.

An optical filter including a near infrared absorbing layer on a glass substrate, in which adhesiveness between the glass substrate and the near infrared absorbing layer is firm, high reliability is enabled in uses under a high temperature and high humidity environment, and manufacturing yield is secured, and a high reliability solid-state imaging device having the optical filter are provided. An optical filter includes: a glass substrate; and a near infrared absorbing layer formed on one principal surface of the glass substrate, wherein the near infrared absorbing layer is obtained by reacting a composition for forming the near infrared absorbing layer on the glass substrate and the composition contains a transparent resin having a fluorene skeleton and a reactivity group; a near infrared absorbing dye; and a silane coupling agent and/or oligomer thereof having a hydrolyzable group and a functional group which have reactivity for the reactivity group.