The present invention provides systems and methods for the decellularization and recellularization of tissue segments. In certain instances the invention comprises coating or sealing decellularized tissue segments with a cross-linked alginate hydrogel. The present invention also provides a decellularization kit, which may be used to generate decellularized tissue segments for high throughput studies. Also included are compositions and methods of tissue sealants comprising methacrylated alginate.

The present invention provides systems and methods for the decellularization and recellularization of tissue segments. In certain instances the invention comprises coating or sealing decellularized tissue segments with a cross-linked alginate hydrogel. The present invention also provides a decellularization kit, which may be used to generate decellularized tissue segments for high throughput studies. Also included are compositions and methods of tissue sealants comprising methacrylated alginate.

Compositions of polymer matrices inclusive of hydrophobically modified and pH-responsive polymers which adhere to a biological tissue for use in delivery of active agents to mammalian subjects are provided.

Compositions of polymer matrices inclusive of hydrophobically modified and pH-responsive polymers which adhere to a biological tissue for use in delivery of active agents to mammalian subjects are provided.

The present disclosure provides biologically-based ink compositions, methods of making the biologically-based ink composition, as well as articles, objects, devices, and/or apparatuses fabricated from or that comprise the biologically-based ink compositions. The biologically-based ink composition can include a silk fibroin solution having a concentration of silk fibroin between 0.1 wt % and 10 wt %, as well as a thickening agent and a humectant dispersed throughout the silk fibroin solution. The biologically-based ink compositions may be used to functionalize a substrate to fabricate sensors, non-toxic conductive inks/textiles, microfluidic channels, technical apparel or fashion accessories, functionalized furniture, tensile canopies, architectural wall paper, facade components, or may be patterned on a substrate to encapsulate scents, flavors, dyes and pigments, therapeutic agents, or biologically active molecules.

The present disclosure provides biologically-based ink compositions, methods of making the biologically-based ink composition, as well as articles, objects, devices, and/or apparatuses fabricated from or that comprise the biologically-based ink compositions. The biologically-based ink composition can include a silk fibroin solution having a concentration of silk fibroin between 0.1 wt % and 10 wt %, as well as a thickening agent and a humectant dispersed throughout the silk fibroin solution. The biologically-based ink compositions may be used to functionalize a substrate to fabricate sensors, non-toxic conductive inks/textiles, microfluidic channels, technical apparel or fashion accessories, functionalized furniture, tensile canopies, architectural wall paper, facade components, or may be patterned on a substrate to encapsulate scents, flavors, dyes and pigments, therapeutic agents, or biologically active molecules.

A system for forming a scaffold-free 3D tissue construct includes a three dimensional (3D) printer; a self-healing, shear thinning, crosslinkable, biocompatible hydrogel support medium; and a first bioink that includes a plurality of cells. The first bioink is capable of being printed with the 3D printer into the hydrogel support medium in a defined shape.

A system for forming a scaffold-free 3D tissue construct includes a three dimensional (3D) printer; a self-healing, shear thinning, crosslinkable, biocompatible hydrogel support medium; and a first bioink that includes a plurality of cells. The first bioink is capable of being printed with the 3D printer into the hydrogel support medium in a defined shape.

The present invention provides systems and methods for the decellularization and recellularization of tissue segments. In certain instances the invention comprises coating or sealing decellularized tissue segments with a cross-linked alginate hydrogel. The present invention also provides a decellularization kit, which may be used to generate decellularized tissue segments for high throughput studies. Also included are compositions and methods of tissue sealants comprising methacrylated alginate.

The present invention provides systems and methods for the decellularization and recellularization of tissue segments. In certain instances the invention comprises coating or sealing decellularized tissue segments with a cross-linked alginate hydrogel. The present invention also provides a decellularization kit, which may be used to generate decellularized tissue segments for high throughput studies. Also included are compositions and methods of tissue sealants comprising methacrylated alginate.